Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206211
R&D Systems製のHuman/Cynomolgus Monkey B7-H1/PD-L1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月27日 08時55分現在]
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[在庫・価格 ：2024年04月27日 08時55分現在]
Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit
文献数： 7
- 商品コード：DB7H10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
アッセイ法：Sandwich，測定範囲：25.00 - 1600 pg/mL，アッセイ数：96，アッセイ時間：4.5時間，検出方法：colorimetric，測定試料：Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)，測定波長：450 nm，種交差性：Human/Cynomolgus Monkey，感度：4.52 pg/mL，別売品コントロール：QC226，測定時間：4.5 hours，測定因子：PD-L1/B7-H1 別名：B7-H Genbank No： 29126 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
VSIG-IGSF11, VISTA-PD-1Hの新規リガンド Quantikine B7-H1/PD-L1 ELISA Kit 免疫チェックポイント特集 Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
- Sensitivity
- 4.52 pg/mL
- Assay Range
- 25.0 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
- Specificity
- Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
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Recovery
The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 97-112 |
| DYC002 (1X) (n=1) | 96 | 90-100 |
| EDTA Plasma (n=4) | 90 | 79-100 |
| Heparin Plasma (n=4) | 85 | 76-95 |
| Serum (n=4) | 89 | 81-100 |
| Urine (n=4) | 109 | 100-116 |
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Linearity
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Additional Infomation
- Entrez Gene IDs
- 29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
- Background
- PD-L1/B7-H1
- PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.
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