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For visualizing lipid droplet, β-oxidation activity & biomembrane in live cells Lipid Metabolism Detection Tools for Cancer Research

Date：January 12 2017Web Page No：95030

Funakoshi Co.,Ltd.

Regulation of lipid metabolism has critical role in cancer cell behavior such as proliferation and survival. Here we offer innovative tools for visualizing lipid metabolism in live cells, lipid storage in lipid droplet (LD) & lipolysis by beta-oxidation.
LipiDye_FAOBlue_LipiDyeM

LipiDye II <Lipid Droplet Green>

LipiDye II is a high sensitive, low cytotoxic and super-photostable fluorescent dye for Lipid Droplets (LDs).
For more detail, please click here.

Features

  • High S/N ratio: Low background in cytoplasm via following two features
    1) Specific accumulation into LDs
    2) Strong green fluorecent emission in non-polar oil phase like LDs
  • High sensitivity: Can observe even very small (<1 μm) LDs
  • Super photo-stability: Long-term live time lapse imaging is possible
  • Low cytotoxicity (at the recommended concentration (0.1～1μM))
  • Compatible with both live and fixed cell
  • Applicable to STED microscopy: can detect less than 500 nm LDs in HeLa cells.

Application Data

  • Staining of various cells (3T3-L1, HepG2, COS-7 and HeLa cells) for 12 hours
    • Staining of various cells with LipiDye II

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FAOBlue <Fatty Acid Oxidation Detection Reagent>

FAOBlue can be easily incorporated into cells and be degraded by mitochondrial fatty acid beta-oxidation (FAO). After FAO cycles, a blue fluorescent dye is released from the degraded reagent to whole cells. FAO inhibitor suppresses a release of the blue fluorescent dye.
For more detail, please click here.

Features

  • Can detect FAO activity directly.
  • Easy procedure with no special equipment.
  • Can detect perturbation of lipid-degradation by drug treatment quantitatively
    → Help to estimate effects of drug candidates on lipid metabolism

Application data