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High sensitive lipid droplets imaging fluorescent dye LipiDye

Date:January 23 2018Web Page No:80682

Funakoshi Co.,Ltd.

LipiDye is a highly sensitive, new fluorescent dye for lipid droplets. Compared to conventional lipid droplet detection dye (Nile Red or boron-dipyrromethene), LipidDye has higher S/N ratio and photo-stability. LipiDye can be used for both live and fixed cell.

Background


Lipid droplets are organelle, which store neutral lipids such as triglycerides and steryl esters, and frequently observed in adipose tissue. It is considered to play a role in energy storage or lipid turnover. Recent studies reported that lipid droplets are not only found in adipocytes, but also in ubiquitous cells such as skeletal muscle cells, macrophages and glia cells. Size of lipid droplets in adipocyte is >10 um and this can be detected by conventional lipid droplet probes like Nile Red. However, size of lipid droplet in non-adipocyte is too small (~0.1 um) to be detected by Nile Red or other probes. LipiDye is a newly developed green fluorescent dye for detecting lipid droplets selectively with high sensitivity, which overcomes a weakness in conventional lipid droplet probes.

Features

  • Selectively incorporated into lipid droplets and gives out green fluorescence.
  • No fluorescence is observed in solution
  • Non specific fluorescence is limited in a cytoplasm.
  • High S/N ratio
  • Compatible with both live and fixed cell
  • Can be used for Live Cell Imaging
  • Low cytotoxicity at normal dose (1 uM)
  • IUPAC name: 1,3-Diphenyl-2-[4-(N,N-diphenylamino)phenyl]benzo[b]phosphole-P-oxide
  • Formulation : C38H28NOP
  • Molecular weight : 545.58 g/mol
  • Solubility : Soluble in DMSO
  • Excitation / Emission: 405 nm / 521-530 nm

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Example Data

Fig. 1 Comparison data between LipiDye and Nile Red
Lipid droplet in Adipocyte is stained with LipiDye or Nile Red. Nile Red also stains cytoplasm, while LipiDye specifically stains lipid droplet clearly.

* General filter for green fluorescent (e.g. FITC or GFP, etc) is not compatible with LipiDye. Please use an appropriate filter.

Fig.2 Preadipocytes differentiation
Differentiation process of preadipocyte (adipose progenitor cell) : 3T3-L1 is observed with LipiDye. Along with differentiation, lipid droplet also becomes grows in size.



Fig.3 Application of LipiDye in both post-fixed and pre-fixed cells
LipiDye is applied to cultured adipocytes after (left) or before (right) paraformaldehyde fixation. Regardless of fixation timing, LipiDye detects lipid droplet highly sensitive.

* Methanol fixation is not recommeded as it affect to the structure of lipid droplets. Please use cross-linking fixatives, such as paraformaldehyde.

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Reference

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Price

[Date : September 22 2018 00:13]

Detail Product Name Product Code Supplier Size Price
LipiDye <Lipid Droplet Green>
Datasheet
FDV-0010 FNAFunakoshi Co.,Ltd. 0.1 mg $200

Description LipiDye is a highly sensitive, new fluorescent dye for lipid droplet. Compared to conventional lipid droplet detection dye (Nile Red or boron-dipyrromethene), LipidDye has higher S/N ratio and photo-stability. LipiDye can be used for both live and fixed cell.
Storage RT CAS
Link

[Date : September 22 2018 00:13]

LipiDye <Lipid Droplet Green>


  • Product Code: FDV-0010
  • Supplier: FNA
  • Size: 0.1mg
  • Price: $200

Description LipiDye is a highly sensitive, new fluorescent dye for lipid droplet. Compared to conventional lipid droplet detection dye (Nile Red or boron-dipyrromethene), LipidDye has higher S/N ratio and photo-stability. LipiDye can be used for both live and fixed cell.
Storage RT CAS
Link

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CONTACT

export@funakoshi.co.jp

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