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HOME> Products> Cell Culture> Coating> iMatrix 411 / iMatrix-221
HOME> Products> Cell Culture> Stem cells> iMatrix 411 / iMatrix-221

Cell Culture Coating Material / Substrate of Laminin Fragment iMatrix 411 / iMatrix-221

Date：March 20 2020Web Page No：81450

Human ES/iPS cells cultured in iMatrix-511 can be induced to differentiate into endothelial progenitor cells by culturing them in iMatrix-411.

iMatrix-221 is an ideal substrate for purification and maintenance culture of cardiomyocytes.

iMatrix Lineup
Product Type
iMatrix-411
iMatrix-221

iMatrix Lineup

iMatrix-511 series for feeder-free culture and single-cell passaging of ES/iPS cells
Liquid format (Dilution required)
Liquid format (No dilution required)
iMatrix-411 for efficient induction of endothelial progenitor cells from iPS cells
Liquid format (Dilution required) These are the products introduced in this article.
iMatrix-221 for purification and maintenance of cardiomyocytes
Liquid format (Dilution required) These are the products introduced in this article.
iMatrix-111 for differentiation-induction into hepatoblast-like cells from human iPS cells
Liquid format (Dilution required)
iMatrix-332 for differentiation-induction into corneal epithelial cells from iPS cells
Liquid format (Dilution required)

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Product Type

	iMatrix 411		iMatrix-221
Code	892041	892042	892061	892062
Package	2x175 µg	6x175 µg	2x175 µg	6x175 µg
Concentration	0.5 mg/mL		175μg/tube
Transgene	Human Laminin-411 E8 fragment		Human Laminin-211 E8 fragment
Expressed in	Recombinant CHO-S cells
Derived from	CHO-S cell culture supernatant
Product grade	For research use
Dissociation constant	10 nM and under
Expiration date	2 years after manufacture

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iMatrix-411

A highly purified product of human laminin-411 E8 fragment produced by CHO cells.
Human ES/iPS cells cultured with iMatrix-511 can be induced to differentiate into endothelial progenitor cells by culturing with iMatrix-411.

Features

Feeder-free culture is also possible.
When used at 0.4 μg/cm², 350 μg (=2x175 µg) can coat about 15 plates of 6-well plate.

Example of Use

iMatrix411 example — Induction of endothelial progenitor cells

Acetyl-LDL: Cholesterol taken up by endothelial progenitor cells
CD31: Endothelial progenitor cells
DAPI: Nucleus

Human ES/iPS cells cultured on iMatrix-511 were differentiated into endothelial progenitor cells by culturing on iMatrix-411, and the cells were confirmed to be functional by LDL uptake assay.

What is Laminin 411-E8 fragment

Laminin 411 is abundant in the basement membrane of blood vessels and is known to bind to integrin α6β1 protein on the cell surface of vascular endothelial cells.
In blood vessels, it is highly expressed along with laminin 511 and 521 proteins, and both are thought to be involved in maintaining vascular homeostasis.
It also plays an important role in blood vessel formation, and it has been reported that newborn pups of laminin 411 knockout mice showed bleeding due to vascular hypoplasia.
It is also known to adhere to leukocytes and platelets, and plays an important role in the immune system and thrombus formation in vivo.
It is also expressed in other cells such as adipocytes.

Reference

Takayama K., et al., Biochemical and Biophysical Research Communications, 474(1), 91-96(2016).
Ohta R., et al., Scientific Reports, 6(35680), 1-12(2016).

Citation

Classifications	References	Topics
Differentiation method of hESC/hPSC	Ohta, et al., Sci. Rep., 6, 35680, (2016)	Endothelial progenitor cells
Differentiation method of hESC/hPSC	Takayama, et al., BBRC. 474(1):91-96, (2016)	Cholangiocytes
Culture for cell line	Tang, et al., BioMed Res. Int., 9465383, 1-10, (2018)	Odontoblast-like cells

Product Information

[Date : February 11 2026 00:11]

Detail Product Name Product Code Supplier Size Price

iMatrix-411
DatasheetThis may not be the latest data sheet. 892041 MAX 2x175 µg $599

Contact

Description
Storage	4°C,Dark Storage	CAS
Link

iMatrix-411
DatasheetThis may not be the latest data sheet. 892042 MAX 6x175 µg $1,546

Contact

Description
Storage	4°C,Dark Storage	CAS
Link

[Date : February 11 2026 00:11]

iMatrix-411

DatasheetThis may not be the latest data sheet.

Product Code: 892041
Supplier: MAX
Size: 2x175µg
Price: $599

Description
Storage	4°C,Dark Storage	CAS
Link

Contact

iMatrix-411

DatasheetThis may not be the latest data sheet.

Product Code: 892042
Supplier: MAX
Size: 6x175µg
Price: $1,546

Description
Storage	4°C,Dark Storage	CAS
Link

Contact

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iMatrix-221

The integrin binding site (E8 fragment) of human laminin-221 protein is purified to high purity. Purification and maintenance culture of cardiomyocytes is possible.

Features

When used at 0.5 μg/cm², 350 μg (=2x175 µg) can coat about 12 plates of 6-well plate.

Video: differentiated cardiomyocytes beating on iMatrix-221

What it Laminin-211 E8 fragment

Laminin-221E8 fragment — Laminin-221 E8 fragment

Laminin is a heterotrimeric molecule consisting of 3 subunit chains: α chain, β chain, and γ chain. There are 5 known types of α chain (α1 to α5), 3 known types of β chain (β1 to β3), and 3 known types of γ chain (γ1 to γ3). In combination, there are more than 12 laminin isoforms.
Laminin 221 is a laminin molecule consisting of α2 chain, β2 chain, and γ1 chain. Laminin 221, along with laminin 211, is abundant in the basement membrane of muscle tissues such as cardiac and skeletal muscle, and is thought to be involved in the differentiation and maintenance of muscle cell function. Laminin 221 and laminin 211 are known to bind to integrin α7X2β1 protein, which is selectively expressed in muscle tissue. It has been reported that laminin 221 has a higher binding affinity to integrin α7X2β1 than laminin 211. It has been reported that integrin α7X2β1 is expressed in cardiac and skeletal muscle cells.

Reference

Nishiuchi, et al, Matrix Biol., 25(3): 189-197 (2006).
Taniguchi, et al, J. Biol. Chem., 284(12): 7820-7831 (2009).
Israeli-Rosenberg, et al, Circ. Res., 114: 572-586 (2014).

Reference

⇒ Please click here

Product Information

[Date : February 11 2026 00:11]

Detail Product Name Product Code Supplier Size Price

iMatrix-221
DatasheetThis may not be the latest data sheet. 892061 MAX 2x175 µg $561

Contact

Description
Storage	4°C,Dark Storage	CAS
Link

iMatrix-221
DatasheetThis may not be the latest data sheet. 892062 MAX 6x175 µg $1,508

Contact

Description
Storage	4°C,Dark Storage	CAS
Link

[Date : February 11 2026 00:11]

iMatrix-221

DatasheetThis may not be the latest data sheet.

Product Code: 892061
Supplier: MAX
Size: 2x175µg
Price: $561

Description
Storage	4°C,Dark Storage	CAS
Link

Contact

iMatrix-221

DatasheetThis may not be the latest data sheet.

Product Code: 892062
Supplier: MAX
Size: 6x175µg
Price: $1,508

Description
Storage	4°C,Dark Storage	CAS
Link

Contact

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