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HOME> Products> Cell Culture> Coating> iMatrix-511 / iMatrix-511 silk
HOME> Products> Cell Culture> Stem cells> iMatrix-511 / iMatrix-511 silk

Suitable substrate for culturing stem cells (ES/iPS cells) iMatrix-511 / iMatrix-511 silk

Date：August 13 2018Web Page No：46137

iMatrix-511 is a high-purity cell culture substrate purified from the E8 fragment of Laminin-511. It enables feeder-free culture and single-cell passaging of ES/iPS cells.
The use of iMatrix-511 for human iPS cell culture is expected to contribute to the simplification of culture procedures^*.

iMatrix-511 Master Cell Bank Change

Date: April 1, 2022

The iMatrix-511 (#892011 and #892012) master cell bank will be changed from May 2022.
For more information, please click here.

* Traditionally, the recommended method for using iMatrix-511 was to coat the container when culturing iPS cells. However, a group led by Associate Professor Hirofumi Suemori of the Institute for Virology and Regenerative Medicine at Kyoto University and Special Assistant Professor Takamichi Miyazaki of the Center for Integrated Material-Cell Systems developed a method for expanding human pluripotent stem cells that does not require coating.

Miyazaki et al. Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner, Scientific Reports, 7(41165), (2017).

Monodispersed human ES cells rapidly adhere and proliferate on Laminin-511 E8 fragments
Human ES cells were passaged with Laminin-511 E8 fragments and other cell culture substrates. When human ES cell colonies were monodispersed during passaging, Laminin-511 E8 fragments rapidly adhered to cells and proliferated.

iMatrix Lineup
Features
Product Type
How to Use
Example of Use
Advantages using Laminin-511 E8 Fragment
What is Laminin-511 E8 fragment
Citation
Product Information

iMatrix Lineup

iMatrix-511 series for feeder-free culture and single-cell passaging of ES/iPS cells
Liquid format (Dilution required) These are the products introduced in this article.
Liquid format (No dilution required)
iMatrix-411 for efficient induction of endothelial progenitor cells from iPS cells
Liquid format (Dilution required)
iMatrix-221 for purification and maintenance of cardiomyocytes
Liquid format (Dilution required)
iMatrix-111 for differentiation-induction into hepatoblast-like cells from human iPS cells
Liquid format (Dilution required)
iMatrix-332 for differentiation-induction into corneal epithelial cells from iPS cells
Liquid format (Dilution required)

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Features

Feeder-free culture is possible.
Single cell passaging is possible.
Can be used for various cells - especially iPS / ES cells
The addition of ROCK inhibitor is not required for medium change after passaging.
We recommend coating with 0.5 μg/cm² for iPS cell production and maintenance culture.
Although iMatrix-511 silk is derived from a different organism than iMatrix-511, it has the same integrin-binding activity and no difference in usage or performance.

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Product Type

Name	iMatrix-511		iMatrix-511 silk
Product appearance
Code	892011	892012	892021
Package	2×175 μg	6×175 μg	6×175 μg
Expressed in/ Produced by	Recombinant CHO-S cells		Recombinant silkworm
Derived from	CHO-S cell culture supernatant		Silkworm cocoon
Product grade	For research use
Transgene	Human Laminin-511 E8 fragment
Purity	95％ and more
Concentration	0.5 mg/mL
Dissociation constant	10 nM and under
Expiration date	2 years after manufacture

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How to Use

Dilute this product with PBS(-) and transfer to a cell culture device so that the concentration becomes 0.1-1.5 μg/cm² *.
Incubate at room temperature for 3 hours and discard the solution.
Add the cells and culture medium and culture the cells.

* The optimum concentration depends on the type of cells.

■ Click here to view the expansion culture protocol for hES/hiPS cells using iMatrix-511.
■ Click here to view a video of hES/hiPS cell passaging using iMatrix-511.

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Example of Use

Evaluation of cell adhesion and spreading effect of Laminin-511 E8 using epidermal cells and vascular endothelial cells

iMatrix-511 (Laminin-511 E8) : Best cell culture substrate for iPS/ES cells!

(a) Most of the epidermal cells were not adherent on uncoated dishes, but many of the cells were spread and adherent on Laminin-511 E8

(b) Vascular endothelial cells adhered to the uncoated petri dish, but many rounded cells were observed. On the other hand, on Laminin-511 E8, few rounded cells were observed, and most cells were well extended.

Comparison of Adhesion Activity of Full-Length Laminin-511 and Laminin-511 E8 Fragment in Human ES Cells

Human ES cells adhere more strongly to "Laminin-511 E8 fragment" than to "full-length Laminin-511"
The cell adhesion strength of Laminin-511 E8 fragment and other cell culture substrates was compared. The horizontal axis represents the concentration of the cell culture substrate, and the vertical axis represents the absorbance at 570 nm. This result confirmed that Laminin-511 E8 fragment had the strongest cell adhesion strength.

Comparison of cell growth in ES/iPS cell cultures using Laminin-511 E8 and Matrigel

Mass Production of ES/iPS Cell Cultures Using Laminin-511 E8 Fragments

The number of ES/iPS cells cultured by the conventional method (colony method) was compared with that of ES/iPS cells cultured using a Laminin-511 E8 fragment as a cell culture substrate for 30 days.
The graph shows the number of days of culture on the horizontal axis and the number of cells on the vertical axis.
The results showed that the number of cells increased more than 200 times when the Laminin-511 E8 fragment was used as a cell culture substrate compared with the conventional method (colony method).

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Advantages using Laminin-511 E8 Fragment

Additional Advantages in ES/iPS Cell Culture Using Laminin-511 E8 Fragments

In conventional culture methods, ES/iPS cells die when they are separated into single cells.
Therefore, passaging must be carried out while maintaining a certain number of cells.

The use of Laminin-511 E8 fragments as cell culture substrates made it possible to maintain single ES/iPS cells.
This reduces the time required to learn cell culture techniques. It also dramatically increases the efficiency of cell culture expansion.

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What is Laminin-511 E8 fragment

(Click here)

iMatrix-511 is a recombinant protein with the same sequence as the Laminin-511 E8 fragment, an enzyme-digested fragment of Laminin-511. Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.
Laminin is composed of three parts; α, β, and γ-chain and 15 types of laminin is already known¹. Among them, Laminin composed by α5-chain, β1-chain, and γ1-chain, is called "Laminin-511".

Cells have integrins, receptors that recognize the extracellular matrix, through which various signals related to cell adhesion, extension, and proliferation. The main receptor that recognizes Laminin-511 is α6β1 integrin. In particular, α6β1 integrin requires the globular domain of the laminin α5 chain and the carboxyl-terminal glutamate of the laminin γ1 chain to recognize Laminin-511^{2, 3}.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion. Although this is fragment, it has binding ability to alpha6beta1 integrin⁴.

Reference

Miner, JH.,et. al., Annu. Rev. Cell Dev. Biol. 20, 255-284 (2004).
Ido, H.,et. al., J. Biol. Chem. 279, 10946-10954 (2004).
Ido, H.,et. al., J. Biol. Chem. 282, 11144-11154 (2007).
Taniguchi, Y.,et. al., J. Biol. Chem. 284, 7820-7831 (2009).
Miyazaki, T., et. al., Nat. Commun. 3, 1236 (2012).

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Citation

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Classification	Documents information	Theme
Establishment and Culture of Human Pluripotent Stem Cells (hPSCs)	Miyazaki, et al., Nat. Commun.3:1236, (2012)	Usefulness of hPSCs as a culture substrate
	Nakagawa, et al., Sci. Rep. 4:3594, (2014)	Establishment of medical-grade hPSCs
	Takashima, et al., Cell.158(6):1254-69, (2014)	Contributes to the transition of hPSCs to the ground state
	Miyazaki, et al., Sci. Rep.7:41165, (2017)	Cultivation of hPSCs by Addition without Coating
	Sekine, et al., Stem Cell Res.24:40-43, (2017)	Establishment of disease-specific hPSCs
	Tan, et al., Stem Cell Res. 24:12-15, (2017)	Establishment of disease-specific hPSCs
	Ishida, et al., Sci. Rep. 8(1), 310, (2018)	Generation of genetic disease models by gene editing of hPSCs
	Kim, et al., Nature Communications, 9(1), 939, (2018)
	Sakai-Takemura, et al., Sci. Rep, 8, 6555, (2018)	Suspension culture of muscle progenitor cells differentiated from hPSCs
	Ling Li, et al., Experimental Neurobiology, 27(5), 350-364, (2018)	Establishment of disease-specific hPSCs from patients; Alzheimer's disease
Cells induced to differentiate from hPSCs	Doi, et al., Stem Cell Reports. 2(3):337-50, (2014)	Dopamine-producing neurons
	Ishikawa, et al., Hum. Mol. Genet.25(23):5188-5197, (2016)
	Nishimura, et al., Stem Cell Reports.6(4):511-524, (2016)
	Samata, et al., Nat. Commun. 7:13097, (2016)
	Kikuchi, et al., Nature. 548(7669):592-596, (2017)
	Morizane, et al., Nat. Commun.8(1):385, (2017)
	Kikuchi, et al., J. Neurosci. Res.95(9):1829-37, (2017)
	Goparaju, et al., Sci. Rep. 7:42367, (2017)	Motor neurons
	Burridge, et al., Nat. Methods.11(8):855-60, (2014)	Cardiomyocytes
	Sougawa, et al., Sci. Rep,8(1), 3726, (2018)	Cardiomyocytes
	Yamauchi, et al., BBRC, 495(1), 1278-1284, (2018)	Ventricular-like cells
	Akiyama, et al., Sci. Rep, 8(1), 1189, (2018)	Skeletal muscle cells
	Saito et al. Stem Cell Res Ther, 9(1), 12, (2018)	Osteoblasts
	Uchimura, et al., Stem cell research, 25, 98-106, (2017)	Myoblasts
	Hayashi, et al., Nature.531(7594):376-80, (2016)	Photoreceptor cells
	Hayashi, et al., Nat. Protoc.12(4):683-696, (2017)	Corneal epithelial cells
	Takayama, et al., BBRC. 474(1):91-96, (2016)	Biliary epithelial cells
	Takayama, et al., Hepatol Commun, 1(10), 1058-1069, (2017)	Hepatocyte-like cells
	Takayama, et al., Biomaterials, (2018)	Hepatocyte-like cells
	Takebe, et al., Cell Reports, 21(10), 2661-2670, (2017)	Hepatoblast
	Tan, et al., Stem Cell Reports, 11:1-11, (2018)	Hepatoblast
	Camp, et al., Nature. 546(7659):533-38, (2017)	Definitive endoderm cells
	Zhang, et al., Stem Cell Reports, 10(2), 1?14, (2018)	Posterior endoderm progenitor cells
	Tanigawa, et al., Cell reports, 15(4), 801-813, (2016)	Nephron progenitor cells (fetal kidney cells)
	Musah, et al., Nat.Biomed.Eng.1:0069, (2017)	Glomerular epithelial cells
	Musah, et al., Nature protocols, 13(7):1662, (2018)	Glomerular epithelial cells
	Mae, et al., BBRC, 495(1), 954-961, (2018)	Ureteric bud
	Oshima, et al., BBRC, 497(2), 719-725, (2018)	Common progenitor cells for blood cells and vascular endothelium
	Taguchi, et al., Cell Stem Cell, 21, (2017)	Culturing hPSCs to differentiate into nephron progenitor cells (fetal kidney cells)
	Kawamura, et al., Stem Cell Reports. 6(3):312-20, (2016)	Culturing hPSCs for cardiomyocyte differentiation
	Sasaki, et al., Cell Stem Cell.17(2):178-94, (2015)	Culture of hPSCs for differentiation into germ cells
	Kojima, et al., Cell Stem Cell.21(4):517-532, (2017)	Culture of hPSCs for differentiation into germ cells
	Furuta, et al., PLoS One. 9(12):e112291, (2014)	Culturing hPSCs for differentiation into mesenchymal stem cells
Primary human cell culture	Okumura, et al., Invest. Ophth. Vis. Sci.56(5):2933-42, (2015)	Human Corneal Endothelial Cells
	Hongo, et al., Invest. Ophth. Vis. Sci. 58(9):3325-34, (2017)	Human Corneal Endothelial Cells
	Polisetti, et al., Sci. Rep.7(1):5152, (2017)	Human limbal epithelial progenitor cells
	Ishii, et al., Stem Cell Reports, 10, 1-15, (2018)	Satellite cell
Molecular mechanisms of laminin-integrin interactions	Ido, et al., J. Biol. Chem. 282(15): 11144-54, (2007)
	Ido, et al., J. Biol. Chem.283(42): 28149-57, (2008)
	Taniguchi, et al., J. Biol. Chem. 284(12): 7820-31, (2009)
	Taniguchi, et al., BBRC.487(3): 525-531, (2017)
	Takizawa, et al., Sci Adv.3(9) :e1701497, (2017)

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Product Information

[Date : February 04 2026 00:08]

Detail Product Name Product Code Supplier Size Price

iMatrix-511 (0.5 mg/mL solution)
DatasheetThis may not be the latest data sheet. 892011 MAX 2x175 µg $290

Contact

Description	The recombinant human Laminin-511 E8 fragment, the coating material / substrate for iPS/ES cells culture
Storage	4°C,Dark Storage	CAS
Link

iMatrix-511 (0.5 mg/mL solution)
DatasheetThis may not be the latest data sheet. 892012 MAX 6x175 µg $787

Contact

Description	The recombinant human Laminin-511 E8 fragment, the coating material / substrate for iPS/ES cells culture.
Storage	4°C,Dark Storage	CAS
Link

iMatrix-511 silk
DatasheetThis may not be the latest data sheet. 892021 MAX 6x175 µg -

Contact

Description	iMatrix-511 silk is a recombinant human laminin-511 E8 fragment protein produced by a silkworm expression system. iMatrix-511 silk is a useful cell culture substrate for feeder-free culture and single-cell passage of ES cells and iPS cells, facilitating stable culture expansion. iMatrix-511 silk is also useful for the culture of other cells adhering to laminin-511.
Storage	4°C,Dark Storage	CAS
Link

[Date : February 04 2026 00:08]

iMatrix-511 (0.5 mg/mL solution)

DatasheetThis may not be the latest data sheet.

Product Code: 892011
Supplier: MAX
Size: 2x175µg
Price: $290

Description	The recombinant human Laminin-511 E8 fragment, the coating material / substrate for iPS/ES cells culture
Storage	4°C,Dark Storage	CAS
Link

Contact

iMatrix-511 (0.5 mg/mL solution)

DatasheetThis may not be the latest data sheet.

Product Code: 892012
Supplier: MAX
Size: 6x175µg
Price: $787

Description	The recombinant human Laminin-511 E8 fragment, the coating material / substrate for iPS/ES cells culture.
Storage	4°C,Dark Storage	CAS
Link

Contact

iMatrix-511 silk

DatasheetThis may not be the latest data sheet.

Product Code: 892021
Supplier: MAX
Size: 6x175µg
Price: -

Description	iMatrix-511 silk is a recombinant human laminin-511 E8 fragment protein produced by a silkworm expression system. iMatrix-511 silk is a useful cell culture substrate for feeder-free culture and single-cell passage of ES cells and iPS cells, facilitating stable culture expansion. iMatrix-511 silk is also useful for the culture of other cells adhering to laminin-511.
Storage	4°C,Dark Storage	CAS
Link

Contact

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