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Best cell culture substrate for iPS/ES cells iMatrix-511 (Laminin-511 E8)

Date:August 13 2018Web Page No:46137

iMatrix-511 (Laminin-511 E8) is beneficial for your stem cell culture!

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells and it can reduce the risk of the xeno-components contamination in the culture. Handling is very easy - just dilute in PBS(-), coat it in the culture dish or plate and incubate at room temparature for 3 hours or at 4°C over night.

Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.

Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".
Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion.
Although this is fragment, it has binding ability to alpha6beta1 integrin.
According to Miyazaki's report ( Nat. Commun. 3, 1236 (2012)), Laminin-511 E8 could be used for cell culture of ES cells and iPS cells.

iMatrix-511 (Laminin-511 E8) : Best cell culture substrate for iPS/ES cells!

To date, mouse feeder cells or matrigel have been used for the culture of iPS cells and ES cells.
However, these cell culture substrates have a risk to bring xeno-components in the culture condition so this was said one of the problems for future medical and clinical application.

iMartix can be used for not only stem cells, but various cells, like epidermal cells, vascular endothelial cells.

iMatrix-511 (Laminin-511 E8) : Best cell culture substrate for iPS/ES cells!

Fig. Human Epidemal Cells and Vascular Endothelical Cells cultured on iMartix-511.
More efficient adhesion and extension can be seen when cultured on iMatrix-511.

Features

  • Simple Handling
  • Can be used for various cells - especially iPS / ES cells
  • Reduce the risk of contamination by xeno-components in your culture system.
  • High cell viability and proliferation rate.
  • 175 μg of iMatrix‐511 can coat about 2 to 6 of 6 well plates.

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Reference

  • Miner, JH.,et. al., Annu. Rev. Cell Dev. Biol. 20,255~284 (2004).
  • Ido, H.,et. al., J. Biol. Chem. 279,10946~10954 (2004).
  • Ido, H.,et. al., J. Biol. Chem. 282,11144~11154 (2007).
  • Taniguchi, Y.,et. al., J. Biol. Chem. 284,7820~7831 (2009).
  • Miyazaki, T., et. al., Nat. Commun. 3,1236 (2012).

[Date : August 13 2018 23:10]

Detail Product Name Product Code Supplier Size Price
iMatrix-511 (0.5 mg/mL solution)
Datasheet
892011 MAX 2x175 µg $280

Description Recombinant Laminin511-E8 fragments. iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Storage 4°C,Dark Strage CAS
Link

iMatrix-511 (0.5 mg/mL solution)
Datasheet
892012 MAX 6x175 µg $720

Description Recombinant Laminin511-E8 fragments. iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Storage 4°C,Dark Strage CAS
Link

[Date : August 13 2018 23:10]

iMatrix-511 (0.5 mg/mL solution)


  • Product Code: 892011
  • Supplier: MAX
  • Size: 2x175µg
  • Price: $280

Description Recombinant Laminin511-E8 fragments. iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Storage 4°C,Dark Strage CAS
Link

iMatrix-511 (0.5 mg/mL solution)


  • Product Code: 892012
  • Supplier: MAX
  • Size: 6x175µg
  • Price: $720

Description Recombinant Laminin511-E8 fragments. iMatrix-511 is produced by CHO-S cells and it can reduce the xeno-components contamination in the culture.
Storage 4°C,Dark Strage CAS
Link

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CONTACT

export@funakoshi.co.jp

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