HOME> Products> Molecular Biology> RNA MW marker> Prestained RNA Ladder

RNA Marker for High (200-8000 base) & Low (20-100 base) Prestained RNA Ladder

Date:February 03 2016Web Page No:46113

Prestain RNA Ladder Marker for single-stranded RNA electrophoresis, made with BioDynamics Laboratory's patented technology.

DynaMarker Prestain Ladder Marker for RNA DynaMarker Prestain Marker for RNA

DynaMarker Prestain Marker for RNA High (#DM260)

DynaMarker Prestain Marker for Small RNA Plus (#DM253)


Colored ladders can solve those problems!

"I wanted to make longer the migration distance a little more."
"I stopped electrophoresis using loading dye as a guide, but target parts were already out of the gel."
"I'm worried if transfer is done properly in blotting."
"After blotting, the inside and outside of a membrane became unrecognizable..."


Low molecular-weight dyes such as bromophenol blue and xylene cyanol have been used as a criterion for monitoring electrophoresis. However, since the mobility of these dyes varies depending on the gel concentration and the electrophoresis conditions, problems such as over-run have been encountered. Especially in valuable samples such as RNA, electrophoresis failure is to be avoided.

Unlike low molecular-weight dyes, DynaMarker Prestain Markers are based on a nucleic-acid chain frame, so there is almost no change in apparent molecular weight due to gel concentration or electrophoresis conditions, and sharp bands can be obtained.
In addition, prestain markers can be transfered to a membrane, so transfer efficiency in Northern blotting can be checked. It also reduces misidentifying the top, bottom, left and right of a membrane.

Prestain RNA ladder transfred on membrane

Features

  • Unlike low-molecular-weight organic-dyes, dyes are introduced directly into chains of nucleic acid by unique dye-binding technique. Therefore, each fragment shows accurate molecular weight even if gel concentration is changed.
  • Separation status can be monitored during electrophoresis, so that you can stop at the perfect timing.
  • Since fragments can transfer to a nylon membrane, it is possible to check whether RNAs are transferred or not.
  • The blue & red (or blue & purple) color scheme provides excellent visibility.
  • Can be used without heating or denaturing step.
  • Prestain marker shows approximate the same mobility as the unstained RNA marker.

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Specification

Name RNA High Small RNA Plus
Code DM260 DM253
Size of bands (base) 200 - 8,000 20 - 100
Colour of bands Blue, Purple Blue, Red
Compatible gel Denaturing agarose Denaturing polyacrylamide
Equivalent unstained RNA marker RNA High
(#DM160, #DM170)
Small RNA Ⅱ
(#DM192)
Application Electrophoresis of
high molecular weight RNA
Coverage to
miRNA precursors

DynaMarker Prestain Ladder Marker for RNA


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Electrophoresis Video

DM253 at 64x speed



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References

#DM253 cited by

  1. Wang, Y., et al., Mol. Genet. Genomics, 290 (2), 471 (2015). [PMID:25293935]
  2. Lin, C. Y., et al., Transgenic Res., 20 (2), 261 (2011). [PMID:20559871]
  3. Ueda T., et al., Genomics, 111 (6), 1183 (2019). [PMID:30223010]

#DM253 featured in

  • “Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs.”
    Iram, S., Methods Mol. Biol., 1099, 179 (2014). [PMID:24243204]

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Product Information

[Date : July 27 2024 00:10]

Detail Product Name Product Code Supplier Size Price
Dynamarker, Prestain Marker for RNA High
DatasheetThis may not be the latest data sheet.
DM260S BDLBioDynamics Laboratory Inc 90 µl $163

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -80°C CAS
Link

Molecular Biology Product List

Dynamarker, Prestain Marker for RNA high
DatasheetThis may not be the latest data sheet.
DM260 BDLBioDynamics Laboratory Inc 180 µl $250

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -80°C CAS
Link

Molecular Biology Product List

DynaMarker, Prestain Marker for Small RNA Plus
DatasheetThis may not be the latest data sheet.
DM253S BDLBioDynamics Laboratory Inc 75 µl $165

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -20°C CAS
Link

Molecular Biology Product List

DynaMarker, Prestain Marker for Small RNA Plus
DatasheetThis may not be the latest data sheet.
DM253 BDLBioDynamics Laboratory Inc 150 µl $250

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -20°C CAS
Link

Molecular Biology Product List

[Date : July 27 2024 00:10]

Dynamarker, Prestain Marker for RNA High


  • Product Code: DM260S
  • Supplier: BDL
  • Size: 90µl
  • Price: $163

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -80°C CAS
Link

Molecular Biology Product List

Dynamarker, Prestain Marker for RNA high


  • Product Code: DM260
  • Supplier: BDL
  • Size: 180µl
  • Price: $250

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -80°C CAS
Link

Molecular Biology Product List

DynaMarker, Prestain Marker for Small RNA Plus


  • Product Code: DM253S
  • Supplier: BDL
  • Size: 75µl
  • Price: $165

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -20°C CAS
Link

Molecular Biology Product List

DynaMarker, Prestain Marker for Small RNA Plus


  • Product Code: DM253
  • Supplier: BDL
  • Size: 150µl
  • Price: $250

Description Prestain RNA ladder marker. Approximately 30 loadings.
Storage -20°C CAS
Link

Molecular Biology Product List

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