HOME> Products> Molecular Biology> Improved Long ssDNA preparation kits

Improved Long ssDNA preparation kits

Date:August 13 2019Web Page No:81351

Specially designed purification kit is now included!
Long single strand DNA (ssDNA) preparation kit
For effective knock-in by genome editing

The Long ssDNA Preparation Kits (LssDNA Preparation Kits) provide a simple and easy method for generating a long ssDNA (<1,500 base or < 3,000 base).
A long ssDNA prepared by this kit has a defined sequence and length as it does not include internal mutation and terminal deletion caused by PCR, exonuclease side reaction, low-fidelity reverse transcriptase reaction or low-fidelity synthetic oligonucleotides.

The procedure is almost the same as the method to obtain dsDNA fragments.
The DNA of interest is cloned into a plasmid. The resulting plasmid harboring the DNA is digested by a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme.
The nicked plasmid is denatured by Denaturing Gel-Loading Buffer and then subjected to agarose gel electrophoresis.
The band corresponding to a long ssDNA is excised and extracted with our newly developed extraction kits.

Features

  • A long ssDNA (< 1,500 base or < 3,000 base) can be prepared.
  • A long ssDNA has a defined sequence and length.
  • Simple principle and easy procedure.
  • High yield and high quality.

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Principle

Principle

(1) The DNA of interest is cloned into a plasmid using a pair of two nicking endonuclease sites or a combination of a nicking endonuclease site and a restriction enzyme site.
(2) The resulting plasmid harboring the DNA is digested with a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme.
(3) The nicked plasmid is denatured and then subjected to agarose gel electrophoresis.
(4) The band corresponding to a long ssDNA is excised and extracted.

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Plasmid Map

Plasmid Map

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Data

Data

Fig.1 Agarose gel electrophoresis of purified long ssDNAs.
1.2% agarose gel in 1x TAE. 5.5cm x 6.0 cm.
Lane 1: pLSODN-1 (1.5 kb Fragment), double-nicked and denatured 800 ng
Lane 2: a long ssDNA purified from pLSODN-1 (1.5 kb Fragment) 400 ng
Lane 3: pLSODN-3 (3 kb Fragment), double-nicked and denatured 800 ng
Lane 4: a long ssDNA purified from pLSODN-3 (3 kb Fragment) 400 ng

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Citation

  1. "ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. "
    Yoshimi K. et al., Nat. Commun., 7, 10431 (2016). Nature Communications. 20;7:10431.

  2. "Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression."
    Liu B., et al., Nat. Immunol., 18 (5), 499 (2017). [PMID:28319097]

  3. "The ER membrane adaptor ERAdP senses the bacterial second messenger c-di-AMP and initiates anti-bacterial immunity."
    Xia P., et al., Nat. Immunol., 19 (2), 141 (2018). [PMID:29292386]

  4. "LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis."
    Zhu P., et al., Nat. Cell. Biol., 20 (10), 1134 (2018). [PMID:30224759]

  5. "Long noncoding RNA lncHand2 promotes liver repopulation via c-Met signaling."
    Wang Y., et al., J Hepatol., 69 (4), 861 (2018). [PMID:29653123]

  6. "IL-13 secreted by ILC2s promotes the self-renewal of intestinal stem cells through circular RNAcircPan3."
    Zhu P., et al., Nat. Immunol., 20 (2), 183 (2019). [PMID:30643264]

  7. "Exonuclease III (XthA) Enforces InVivo DNA Cloning of Escherichia coli To Create CohesiveEnds."
    Nozaki S. & Niki H., J. Bacteriol., 201 (5), e00660-18 (2019). [PMID:30530516]

  8. "New Additions to the CRISPR Toolbox: CRISPR- CLONInG and CRISPR- CLIP for Donor Construction in Genome Editing."
    Shola D.T.N., et al., CRISPR J., 3 (2), 109 (2020). [PMID:32315232]

  9. "Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor."
    Ranawakage D.C., et al., Front. Cell. Dev. Biol., 8, 598634 (2021). [PMID:33681181]

  10. "Oocyte-specific gene knockdown by intronic artificial microRNAs driven by Zp3 transcription in mice."
    Sasaki K., et al., J. Reprod. Dev., 67 (3), 229 (2021). [PMID:33716236]

  11. "Hepatitis B virus cccDNA is formed through distinct repair processes of each strand."
    Wei L., et al., Nat. Commun., 12 (1), 1591 (2021). [PMID:33707452]


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Kit Contents

DS615 Long ssDNA Preparation Kit for 1.5 kb (LsODN Preparation Kit)
• pLSODN-1 10 μg (0.5 μg/μl)
• pLSODN-2D 10 μg (0.5 μg/μl)
• Denaturing Gel-Loading Buffer 1 ml (100 loadings)
• Nicked pLSODN-1 (1.5 kb Fragment)
• Long ssDNA Gel Extraction Kit for 3kb (25 preps)

DS625 Long ssDNA Preparation Kit for 3.0 kb (LsODN Preparation Kit)
• pLSODN-3 10 μg (0.5 μg/μl )
• pLSODN-4D 10 μg (0.5 μg/μl )
• Denaturing Gel-Loading Buffer 1 ml (100 loadings)
• Nicked pLSODN-3 (3.0 kb Fragment)
• Long ssDNA Gel Extraction Kit for 3kb (25 preps)

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Attention!

(1) LssDNA kit has two price systems now (Academic or Industrial).
Please contact your local distributor about price.
(2) Letter of Assurance (LoA) is required upon ordering.

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Product Information

For 1.5kb

[Date : June 19 2024 00:06]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Preparation Kit for 1.5kb, Academic
DatasheetThis may not be the latest data sheet.
DS615 BDLBioDynamics Laboratory Inc 1 kit $700

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long ssDNA Preparation Kit for 1.5kb, Commercial Entities
DatasheetThis may not be the latest data sheet.
DS615 BDLBioDynamics Laboratory Inc 1 kit $1,400

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

[Date : June 19 2024 00:06]

Long ssDNA Preparation Kit for 1.5kb, Academic


  • Product Code: DS615
  • Supplier: BDL
  • Size: 1kit
  • Price: $700

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long ssDNA Preparation Kit for 1.5kb, Commercial Entities


  • Product Code: DS615
  • Supplier: BDL
  • Size: 1kit
  • Price: $1,400

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

For 3.0kb

[Date : June 19 2024 00:06]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Preparation Kit for 3kb, Academic
DatasheetThis may not be the latest data sheet.
DS625 BDLBioDynamics Laboratory Inc 1 kit $700

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long ssDNA Preparation Kit for 3kb, Commercial Entities
DatasheetThis may not be the latest data sheet.
DS625 BDLBioDynamics Laboratory Inc 1 kit $1,400

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

[Date : June 19 2024 00:06]

Long ssDNA Preparation Kit for 3kb, Academic


  • Product Code: DS625
  • Supplier: BDL
  • Size: 1kit
  • Price: $700

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long ssDNA Preparation Kit for 3kb, Commercial Entities


  • Product Code: DS625
  • Supplier: BDL
  • Size: 1kit
  • Price: $1,400

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link


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Related Product

Gel Extraction Kit

[Date : June 19 2024 00:06]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Gel Extraction Kit for 3kb
DatasheetThis may not be the latest data sheet.
DS640 BDLBioDynamics Laboratory Inc 25 tests $300

Description Long ssDNA Gel Extraction Kit for 1.5 - 3kb.
Storage RT CAS
Link

[Date : June 19 2024 00:06]

Long ssDNA Gel Extraction Kit for 3kb


  • Product Code: DS640
  • Supplier: BDL
  • Size: 25tests
  • Price: $300

Description Long ssDNA Gel Extraction Kit for 1.5 - 3kb.
Storage RT CAS
Link


10 kb single strand DNA preparation kit

[Date : June 19 2024 00:06]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Preparation Kit for 10kb, Academic
DatasheetThis may not be the latest data sheet.
DS635 BDLBioDynamics Laboratory Inc 1 kit $1,000

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 10,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-80°C CAS
Link

10 kb single strand DNA preparation kit

Long ssDNA Preparation Kit for 10kb, Commercial Entities
DatasheetThis may not be the latest data sheet.
DS635 BDLBioDynamics Laboratory Inc 1 kit $2,000

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 10,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-80°C CAS
Link

10 kb single strand DNA preparation kit

[Date : June 19 2024 00:06]

Long ssDNA Preparation Kit for 10kb, Academic


  • Product Code: DS635
  • Supplier: BDL
  • Size: 1kit
  • Price: $1,000

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 10,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-80°C CAS
Link

10 kb single strand DNA preparation kit

Long ssDNA Preparation Kit for 10kb, Commercial Entities


  • Product Code: DS635
  • Supplier: BDL
  • Size: 1kit
  • Price: $2,000

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 10,000 base). Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-80°C CAS
Link

10 kb single strand DNA preparation kit

Go back to index

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