HOME> Products> Molecular Biology> Improved Long ssDNA preparation kits

Improved Long ssDNA preparation kits

Date:August 13 2019Web Page No:81351

Specially designed purification kit is now included!
Long single strand DNA (ssDNA) preparation kit
For effective knock-in by genome editing

The Long ssDNA Preparation Kits (LssDNA Preparation Kits) provide a simple and easy method for generating a long ssDNA (<1,500 base or < 3,000 base).
A long ssDNA prepared by this kit has a defined sequence and length as it does not include internal mutation and terminal deletion caused by PCR, exonuclease side reaction, low-fidelity reverse transcriptase reaction or low-fidelity synthetic oligonucleotides.

The procedure is almost the same as the method to obtain dsDNA fragments.
The DNA of interest is cloned into a plasmid. The resulting plasmid harboring the DNA is digested by a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme.
The nicked plasmid is denatured by Denaturing Gel-Loading Buffer and then subjected to agarose gel electrophoresis.
The band corresponding to a long ssDNA is excised and extracted with our newly developed extraction kits.

Features

  • A long ssDNA (< 1,500 base or < 3,000 base) can be prepared.
  • A long ssDNA has a defined sequence and length.
  • Simple principle and easy procedure.
  • High yield and high quality.

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Principle

Principle

(1) The DNA of interest is cloned into a plasmid using a pair of two nicking endonuclease sites or a combination of a nicking endonuclease site and a restriction enzyme site.
(2) The resulting plasmid harboring the DNA is digested with a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme.
(3) The nicked plasmid is denatured and then subjected to agarose gel electrophoresis.
(4) The band corresponding to a long ssDNA is excised and extracted.

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Plasmid Map

Plasmid Map

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Data

Data

Fig.1 Agarose gel electrophoresis of purified long ssDNAs.
1.2% agarose gel in 1x TAE. 5.5cm x 6.0 cm.
Lane 1: pLSODN-1 (1.5 kb Fragment), double-nicked and denatured 800 ng
Lane 2: a long ssDNA purified from pLSODN-1 (1.5 kb Fragment) 400 ng
Lane 3: pLSODN-3 (3 kb Fragment), double-nicked and denatured 800 ng
Lane 4: a long ssDNA purified from pLSODN-3 (3 kb Fragment) 400 ng

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Citation

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T. (2016)
Nature Communications. 20;7:10431.

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Kit Contents

DS615 Long ssDNA Preparation Kit for 1.5 kb (LsODN Preparation Kit)
• pLSODN-1 10 μg (0.5 μg/μl)
• pLSODN-2D 10 μg (0.5 μg/μl)
• Denaturing Gel-Loading Buffer 1 ml (100 loadings)
• Nicked pLSODN-1 (1.5 kb Fragment)
• Long ssDNA Gel Extraction Kit for 3kb (25 preps)

DS625 Long ssDNA Preparation Kit for 3.0 kb (LsODN Preparation Kit)
• pLSODN-3 10 μg (0.5 μg/μl )
• pLSODN-4D 10 μg (0.5 μg/μl )
• Denaturing Gel-Loading Buffer 1 ml (100 loadings)
• Nicked pLSODN-3 (3.0 kb Fragment)
• Long ssDNA Gel Extraction Kit for 3kb (25 preps)

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Attention!

(1) LssDNA kit has two price systems now (Academic or Industrial).
Please contact your local distributor about price.
(2) Letter of Assurance (LoA) is required upon ordering.

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Product Information

[Date : August 22 2019 23:13]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Preparation Kit for 1.5kb, Academic
DatasheetThis may not be the latest data sheet.
DS615 BDLBioDynamics Laboratory Inc 1 kit $620

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 1.5kb, Commercial Entities
DatasheetThis may not be the latest data sheet.
DS615 BDLBioDynamics Laboratory Inc 1 kit $1,240

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 3kb, Academic
DatasheetThis may not be the latest data sheet.
DS625 BDLBioDynamics Laboratory Inc 1 kit $620

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 3kb, Commercial Entities
DatasheetThis may not be the latest data sheet.
DS625 BDLBioDynamics Laboratory Inc 1 kit $1,240

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

[Date : August 22 2019 23:13]

Long ssDNA Preparation Kit for 1.5kb, Academic


  • Product Code: DS615
  • Supplier: BDL
  • Size: 1kit
  • Price: $620

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 1.5kb, Commercial Entities


  • Product Code: DS615
  • Supplier: BDL
  • Size: 1kit
  • Price: $1,240

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 3kb, Academic


  • Product Code: DS625
  • Supplier: BDL
  • Size: 1kit
  • Price: $620

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Long ssDNA Preparation Kit for 3kb, Commercial Entities


  • Product Code: DS625
  • Supplier: BDL
  • Size: 1kit
  • Price: $1,240

Description The Long ssDNA (LssDNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides. <Note 1> Letter of Assurance (LoA) is required for purchase. Please ask your local distributor or Funakoshi. <Note 2> For commercial purpose use, license contract should be made between customers and Funakoshi. Please ask Funakoshi for detail.
Storage RT,-20°C CAS
Link

Long Single Stranded DNA Preparation Kit

Go back to index

Related Product

[Date : August 22 2019 23:13]

Detail Product Name Product Code Supplier Size Price
Long ssDNA Gel Extraction Kit for 3kb
DatasheetThis may not be the latest data sheet.
DS640 BDLBioDynamics Laboratory Inc 25 tests $300

Description Long ssDNA Gel Extraction Kit for 1.5 - 3kb.
Storage RT CAS
Link

Long Single Stranded DNA Preparation Kit

[Date : August 22 2019 23:13]

Long ssDNA Gel Extraction Kit for 3kb


  • Product Code: DS640
  • Supplier: BDL
  • Size: 25tests
  • Price: $300

Description Long ssDNA Gel Extraction Kit for 1.5 - 3kb.
Storage RT CAS
Link

Long Single Stranded DNA Preparation Kit

Go back to index

CONTACT

export@funakoshi.co.jp

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  • Please note that Product Information or Price may change without notice.