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HOME> Products> Molecular Biology> Enzyme / Enzyme Inhibitor> T7 Endonuclease I

Useful for checking mutation introduction by genome editing technology T7 Endonuclease I

Date：June 02 2025Web Page No：160486

T7 Endonuclease I is a mismatch DNA cleavage enzyme that detect the editing efficiency of genomic DNA. The product contains enough enzyme for 50 reactions in a 10 μl reaction volume.

What is T7 Endonuclease I?
Featrues
Example of Use
Bibliography
Set Contents
Product Information

What is T7 Endonuclease I?

T7 Endonuclease I (T7EI / T7E1) is a homodimeric enzyme composed of 149-amino-acid subunits derived from T7 phage. In addition to resolving four-way Holliday junctions—crucial intermediates formed during both homologous recombination and site-specific recombination—T7EI exhibits cleavage activity against a variety of mismatched DNA structures, ranging from branched DNA to heteroduplexes with base mismatches. This characteristic allows for the detection of DNA mutations based on the presence or absence of DNA cleavage.

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Featrues

The cleavage assay is completed in just 15 minutes.
The digestion control is included.
The product contains enough enzyme for 50 reactions, including control reactions.

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Example of Use

The digestion control was nicked using T7EI.

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Bibliography

Guan, C. and Kumar, S., Nucleic Acids Res., 33 (19), 6225-6234 (2005). [PMID:16264086]
Arvind, L., et al., Nucleic Acids Res., 28 (18), 3417-3432 (2000). [PMID:10982859]
Mashal, R.D., et al., Nature Genetics, 9 (2), 177-183 (1995). [PMID:7719346]

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Set Contents

10× T7 Endonuclease I
10× Digestion Buffer
Digestion Control

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Product Information

[Date : June 29 2025 00:07]

Detail Product Name Product Code Supplier Size Price

T7 Endonuclease I
DatasheetThis may not be the latest data sheet. DS715 BDLBioDynamics Laboratory Inc 1 set $150

Contact

Description	A set containing T7 Endonuclease I, reaction buffer, and a cleavage control (sufficient for 50 reactions). T7 Endonuclease I exhibits cleavage activity not only toward four-way Holliday junctions but also a wide range of mismatched DNA structures, including branched DNA and heteroduplexes with base mismatches.
Storage	-20°C	CAS
Link

[Date : June 29 2025 00:07]

T7 Endonuclease I

DatasheetThis may not be the latest data sheet.

Product Code: DS715
Supplier: BDL
Size: 1set
Price: $150

Description	A set containing T7 Endonuclease I, reaction buffer, and a cleavage control (sufficient for 50 reactions). T7 Endonuclease I exhibits cleavage activity not only toward four-way Holliday junctions but also a wide range of mismatched DNA structures, including branched DNA and heteroduplexes with base mismatches.
Storage	-20°C	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

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