Useful for checking mutation introduction by genome editing technology T7 Endonuclease I
Date:June 02 2025Web Page No:160486
T7 Endonuclease I is a mismatch DNA cleavage enzyme that detect the editing efficiency of genomic DNA. The product contains enough enzyme for 50 reactions in a 10 μl reaction volume.
- What is T7 Endonuclease I?
- Featrues
- Example of Use
- Bibliography
- Set Contents
- Product Information
- Related Products
What is T7 Endonuclease I?
T7 Endonuclease I (T7EI / T7E1) is a homodimeric enzyme composed of 149-amino-acid subunits derived from T7 phage. In addition to resolving four-way Holliday junctions—crucial intermediates formed during both homologous recombination and site-specific recombination—T7EI exhibits cleavage activity against a variety of mismatched DNA structures, ranging from branched DNA to heteroduplexes with base mismatches. This characteristic allows for the detection of DNA mutations based on the presence or absence of DNA cleavage.
Featrues
- The cleavage assay is completed in just 15 minutes.
- The digestion control is included.
- The product contains enough enzyme for 50 reactions, including control reactions.
Example of Use
Bibliography
Set Contents
- T7 Endonuclease I (10×)
- Digestion Buffer (10×)
- Digestion Control
* Nuclease-free water is required separately. Please refer to the datasheet for required materials.
Product Information
[Date : August 18 2025 00:07]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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T7 Endonuclease I DatasheetThis may not be the latest data sheet. |
DS715 | BDLBioDynamics Laboratory Inc | 1 set | $150 | |||||||||||||||||||||||||||||||
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[Date : August 18 2025 00:07]
T7 Endonuclease I
DatasheetThis may not be the latest data sheet.
- Product Code: DS715
- Supplier: BDL
- Size: 1set
- Price: $150
Description |
A set containing T7 Endonuclease I, reaction buffer, and a cleavage control (sufficient for 50 reactions). T7 Endonuclease I exhibits cleavage activity not only toward four-way Holliday junctions but also a wide range of mismatched DNA structures, including branched DNA and heteroduplexes with base mismatches. |
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Storage | -20°C | CAS | |
Link |
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Related Products
■ Long ssDNA Preparation Kit for 1.5kb & 3.0kb
This kit can prepare long single-stranded DNA (ssDNA, 1,500 base or 3,000 base) with high purity and accurate sequence by Nicking Enzyme Amplification Reaction.
■ Long ssDNA Preparation Kit for 10kb
Challenging the limits of long single-stranded DNA! Single-stranded DNA up to 10 kb can be prepared.
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