抗LAP抗体(Anti-LAP, TGF-β1, Human, Mouse-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)Leu30-Arg278 (Cys33Ser)Accession # P01137
Source	Monoclonal Mouse IgG2A Clone # 27240
Purification	Protein A or G purified from ascites
Specificity	Recognizes human LAP (TGF-beta 1) and human latent TGF-beta 1 in direct ELISAs.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Human LAP TGF-beta 1 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human LAP (TGF-beta 1) Antibody (Catalog #MAB2461 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human LAP TGF-beta 1 Biotinylated Antibody (Catalog #BAM2462 )
ELISA Standard		Recombinant Human LAP (TGF-beta 1) Protein (Catalog #246-LP )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)	The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopussystems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3,TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form ofan inactive complex, referred to as latent TGF-beta, that consists ofTGF-beta associated non-covalently with LAP. These two proteins aresynthesized as a single pro-peptide that is cleaved in a post Golgicompartment prior to secretion. Different TGF-beta family members arenaturally associated with their own distinct LAPs.
Long Name:	Latency-associated Peptide
Entrez Gene IDs:	7040 (Human)
Alternate Names:	CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

目次に戻る

Related Research Areas

Airway Inflammation and Remodeling

Cytokines and Receptors in Angiogenesis

gamma delta T Cells

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Platelet Cytokine and Growth Factor Receptors

T Cell Cytokine Signaling

TGF-beta Family

TGF-beta Family Ligands

Th17 Cells

Th22 Cells

Th9 Cells

Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans. Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS J. Immunol., 2006;177(9):5811-8. Species: Human Sample Type: Whole Cells Application: Flow

目次に戻る

Related Product & Information

Background

LAP (TGF-beta 1)

background_content

Background: LAP (TGF-beta 1) The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopussystems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3,TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form ofan inactive complex, referred to as latent TGF-beta, that consists ofTGF-beta associated non-covalently with LAP. These two proteins aresynthesized as a single pro-peptide that is cleaved in a post Golgicompartment prior to secretion. Different TGF-beta family members arenaturally associated with their own distinct LAPs.

目次に戻る

Citations

1. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
   Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
   J. Immunol., 2006;177(9):5811-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
   Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
   Blood, 2005;105(2):750-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Flow


3. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice.
   Authors: Kitani A, Fuss I, Pedersen A, Nawata H
   J. Immunol., 2004;172(2):834-42.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る