抗2B4抗体(Anti-2B4, Human, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：29593
2B4に対する抗体(Anti-2B4, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-2B4, Human, Goat-Poly <Anti-CD244> |
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Anti-Human 2B4/CD244/SLAMF4 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-2B4, Human, Goat-Poly <Anti-CD244>
文献数： 2
- 商品コード：AF1039
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | NK細胞活性化作用を有する。マッチドペア：Human 2B4/CD244/SLAMF4 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1039，スタンダードとして#1039-2B-050を用いる。 別名：CD244 Genbank No： 51744 Protein Accession No： NP_057466 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human 2B4/CD244/SLAMF4 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1039-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD244 Genbank No： 51744 Protein Accession No： NP_057466 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human 2B4/CD244/SLAMF4Cys22-Arg221Accession # NP_057466 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human 2B4/CD244/SLAMF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse 2B4, recombinant human (rh) SLAM, rhCD48, and rhCD84 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| Immunohistochemistry | 5-15 µg/mL | See below |
| Agonist Activity | Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED50 for this effect is typically 4‑12 µg/mL. | |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Human 2B4/CD244/SLAMF4 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human 2B4/CD244/SLAMF4 Antibody (Catalog #AF1039 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human 2B4/CD244/SLAMF4 Biotinylated Antibody (Catalog #BAF1039 ) |
| ELISA Standard | Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera Protein, CF (Catalog #1039-2B ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot 2B4/CD244/SLAMF4 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/2B4_AF1039_Western_Blot_21759.jpg) click image to view larger | Detection of Human 2B4/CD244/SLAMF4 by Western Blot. Western blot shows lysates of NK‑RL human natural killer lymphocytic leukemia cell line, NK‑92 human natural killer lymphoma cell line, and Daudi human Burkitt's lymphoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry |
![Flow Cytometry 2B4/CD244/SLAMF4 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/2B4_AF1039_Flow_Cytometry_18286.jpg) click image to view larger | Detection of 2B4/CD244/SLAMF4 in Human PBMCs by Flow Cytometry.Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
| Immunohistochemistry |
![Immunohistochemistry 2B4/CD244/SLAMF4 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/2B4_AF1039_Immunohistochemistry_20200.jpg) click image to view larger | 2B4/CD244/SLAMF4 in Human Spleen. 2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: 2B4/CD244/SLAMF4 | 2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1‑4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%‑24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15). | |
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References
| Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417. | |
| Veillette, A. (2006) Nat. Rev. Immunol. 6:56. | |
| McNerney, M.E. et al. (2005) Mol. Immunol. 42:489. | |
| Assarsson, E. et al. (2005) J. Immunol. 175:2045. | |
| Boles, K.S. et al. (1999) Tissue Antigens 54:27. | |
| Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466. | |
| Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676. | |
| Lee, K.M. et al. (2006) Blood 107:3181. | |
| Mooney, J.M. et al. (2004) J. Immunol. 173:3953. | |
| Assarsson, E. et al. (2004) J. Immunol. 173:174. | |
| Bryceson, Y.T. et al. (2006) Blood 107:159. | |
| Lee, K-M. et al. (2004) J. Exp. Med. 199:1245. | |
| McNerney, M.E. et al. (2005) Blood 106:1337. | |
| Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870. | |
| Messmer, B. et al. (2006) J. Immunol. 176:4646. | |
| Entrez Gene IDs: | 51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey) |
| Alternate Names: | 2B4; CD244 antigen; CD244 molecule, natural killer cell receptor 2B4; CD244; h2B4; NAIL; NAILNK cell activation-inducing ligand; natural killer cell receptor 2B4; NKR2B4; NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4; Nmrk; SLAMF4; SLAMF4NK cell activation inducing ligand NAIL |
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Related Research Areas
| Basophils | ||
| Co-stimulatory and Co-inhibitory Molecules | ||
| Hematopoietic Stem Cell Markers | ||
| ITIM/ITAM Immunoreceptors and Related Molecules | ||
| Monocyte Markers | ||
| ||
| Western Blot | |
|---|---|
 | Detection of Human 2B4/CD244/SLAMF4 by Western Blot. Western blot shows lysates of NK‑RL human natural killer lymphocytic leukemia cell line, NK‑92 human natural killer lymphoma cell line, and Daudi human Burkitt's lymphoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for 2B4/CD244/SLAMF4 at approximately 65 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry | |
 | Detection of 2B4/CD244/SLAMF4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
| Immunohistochemistry | |
 | 2B4/CD244/SLAMF4 in Human Spleen. 2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Related Product & Information
| Entrez Gene IDs | 51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey) |
|---|---|
| Background | 2B4/CD244/SLAMF4 |
| background_content | Background: 2B4/CD244/SLAMF4 2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1‑4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%‑24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells.
Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V
J. Immunol., 2009;183(7):4792-9.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
Proc. Natl. Acad. Sci. U.S.A., 2006;103(30):11258-63.
Species: Human
Sample Type: Whole Cells
Application: ICC
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