抗Mouse IL-10抗体(Anti-Mouse IL-10 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-10Accession # P18893
Source	Monoclonal Rat IgG1 Clone # JES052A5
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL‑10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL‑10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Mouse IL-10 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑10 Antibody (Catalog #MAB417 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑10 Biotinylated Antibody (Catalog #BAF417 )
ELISA Standard		Recombinant Mouse IL-10 Protein (Catalog #417-ML )
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.015 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog # 417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization	click image to view larger	Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody.Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417). The ND50 is typically 0.005-0.015 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10	Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Long Name:	Interleukin 10
Entrez Gene IDs:	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names:	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Related Research Areas

B Cell Development and Differentiation

B Cell Markers

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

IL-10/Interferon Family

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Lymphoma

Macrophage Activation Markers

Molecules Secreted by VSMC

Monocyte Markers

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

Neuroinflammation

Plasmacytoid Dendritic Cells

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Th2 Cells

Th22 Cells

Th9 Cells

Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury Authors: S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd J Neuroinflammation, 2018;15(1):90. Species: Rat Sample Type: Whole Cells Application: Neut

Western Blot
	Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog # 417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417). The ND50 is typically 0.005-0.015 µg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

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Citations

1. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury
   Authors: S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd
   J Neuroinflammation, 2018;15(1):90.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


2. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
   Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
   J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. PI3Kgamma activates integrin alpha4 and promotes immune suppressive myeloid cell polarization during tumor progression
   Authors: JA Varner, P Foubert, MM Kaneda
   Cancer Immunol Res, 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. A critical role for IRF5 in regulating allergic airway inflammation
   Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
   Mucosal Immunol, 2017;10(3):716-726.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


5. CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract
   Authors: LX Li, JC Labuda, DM Imai, SM Griffey, SJ McSorley
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


6. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
   Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
   Nat Commun, 2017;8(0):14919.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


7. Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
   Authors: Yaoqin Hu
   Mediators Inflamm, 2016;2016(0):3735452.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
   Authors: Jung-Yeon Lim
   Sci Rep, 2016;6(0):26851.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


9. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
   Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
   J Immunol, 2015;194(1):334-48.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Development


10. Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
    Authors: McKelvey R, Berta T, Old E, Ji R, Fitzgerald M
    J Neurosci, 2015;35(2):457-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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