抗IL-10抗体(Anti-IL-10, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10Ser19-Asn178Accession # P22301
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant Epstein-Barr virus IL-10 is observed and less than 20% cross-reactivity with recombinant mouse IL‑10, recombinant feline IL-10, recombinant porcine IL‑10, recombinant equine IL-10,  and recombinant rat IL‑10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Western Blot
	Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	IL‑10 in Human Tonsil. IL‑10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	IL‑10 in Human Tonsil. IL‑10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑217‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human IL‑10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization
	Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA). The ND50 is typically 0.2-1.0 µg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL-10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (11‑13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

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Citations

1. Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps
   PLoS ONE, 2016;11(9):e0161013.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
   Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
   Leukemia, 2016;30(1):163-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Expression of Tim-1 in primary CNS lymphoma
   Cancer Med, 2016;5(11):3235-3245.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


4. Immune suppression and resistance mediated by constitutive activation of Wnt/beta-catenin signaling in human melanoma cells.
   J. Immunol., 2012;189(5):2110-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. IL-10 expression by primary tumor cells correlates with melanoma progression from radial to vertical growth phase and development of metastatic competence.
   Authors: Itakura E, Huang RR, Wen DR, Paul E, Wunsch PH, Cochran AJ
   Mod. Pathol., 2011;24(6):801-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
   Authors: Ouwehand K, Oosterhoff D, Breetveld M
   J. Invest. Dermatol., 2011;131(2):418-25.
   Species: Human
   Sample Type: Whole Tissue
   Application: Neut


7. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
   Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
   Blood, 2010;116(12):2061-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


8. Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
   Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
   Mucosal Immunol, 2008;1(5):412-20.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007;109(11):4856-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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