抗IL-10抗体(Anti-IL-10, Canine, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27883
IL-10に対する抗体(Anti-IL-10, Canine, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-IL-10, Canine, Goat-Poly, Biotin
文献数： 4
- 商品コード：BAF735
- メーカー：RSD
- 包装：50μg
- 価格：¥114,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Canine IL-10 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB7351，スタンダードとして#735-CL-010を用いる。 別名：CSIF Genbank No： 3586 Protein Accession No： XP_855560 |
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| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Canine | 免疫動物 | Goat |
| 交差性 | Canine | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Canine |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant canine IL‑10 (R&D Systems, Catalog # 735-CL)Ser20-Ile179Accession # XP_855560 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects canine IL-10 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant feline (rfe) IL‑10, recombinant human (rh) IL-10, recombinant mouse (rm) IL-10, recombinant porcine (rp) IL-10, and recombinant rat (rr) IL-10 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Canine IL‑10 (Catalog # 735-CL) |
| Canine IL-10 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Canine IL‑10 Antibody (Catalog #MAB7351 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Canine IL‑10 Biotinylated Antibody (Catalog #BAF735 ) |
| ELISA Standard | Recombinant Canine IL-10 Protein (Catalog #735-CL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-10 | IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The canine homologue of mouse IL-10 has been cloned. Canine IL-10 shares 80% amino acid sequence homology with human IL-10 and 72% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. |
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References
| Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165. | |
| Long Name: | Interleukin 10 |
| Entrez Gene IDs: | 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral) |
| Alternate Names: | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF |
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Related Research Areas
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Related Product & Information
| Background | IL-10 |
|---|---|
| background_content | Background: IL-10 IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones. The canine homologue of mouse IL-10 has been cloned. Canine IL-10 shares 80% amino acid sequence homology with human IL-10 and 72% sequence homology with mouse IL-10. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. |
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Citations
- A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden
Authors: BM Roatt, RD Aguiar-Soa, LE Reis, JM Cardoso, FA Mathias, RC de Brito, SM da Silva, NF Gontijo, SA Ferreira, JG Valenzuela, R Corrêa-Oli, RC Giunchetti, AB Reis
Front Immunol, 2017;8(0):217.
Species: Canine
Sample Type: Cell Culture Supernates
Application: ELISA detection - Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
PLoS Negl Trop Dis, 2016;10(5):e0004614.
Species: Canine
Sample Type: Cell Culture Supernates
Application: ELISA detection - Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs.
Authors: Haurigot V, Mingozzi F, Buchlis G
Mol. Ther., 2010;18(7):1318-29.
Species: Canine
Sample Type: Whole Cells
Application: Flow - Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
Vaccine, 2005;24(4):501-9.
Species: Canine
Sample Type: Cell Culture Supernates
Application: ELISA Development
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