抗IL-10抗体(Anti-IL-10, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27739
IL-10に対する抗体(Anti-IL-10, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-IL-10, Rat, Goat-Poly, Biotin
文献数： 1
- 商品コード：BAF519
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat IL-10 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB519-500，スタンダードとして#522-RL-005を用いる。 別名：CSIF Genbank No： 3586 Protein Accession No： P29456 |
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| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat IL‑10Ser19-Asn178Accession # P29456 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat IL-10 in ELISAs and Western blots. In sandwich ELISAs, less than 35% cross-reactivity with recombinant mouse (rm) IL-10 is observed and 0.2% cross-reactivity with recombinant human (rh) IL-10, recombinant porcine IL-10, recombinant canine IL-10 and recombinant feline IL-10 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat IL‑10 (Catalog # 522-RL) |
| Rat IL-10 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat IL‑10 Antibody (Catalog #MAB519 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat IL‑10 Biotinylated Antibody (Catalog #BAF519 ) |
| ELISA Standard | Recombinant Rat IL-10 Protein (Catalog #522-RL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-10 | Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2). |
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References
| Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. | |
| O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425. | |
| Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. | |
| Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. | |
| Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. | |
| Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. | |
| Hsu, D.-H. et al. (1990) Science 250:830. | |
| Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1. | |
| Windsor, W.T. et al. (1993) Biochemistry 32:8807. | |
| Syto, R. et al. (1998) Biochemistry 37:16943. | |
| Kotenko, S.V. et al. (1997) EMBO J. 16:5894. | |
| Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725. | |
| Hor, S. et al. (2004) J. Biol. Chem. 279:33343. | |
| Sheppard, P. et al. (2003) Nat. Immunol. 4:63. | |
| Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. | |
| Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. | |
| Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143. | |
| Long Name: | Interleukin 10 |
| Entrez Gene IDs: | 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral) |
| Alternate Names: | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF |
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Related Research Areas
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Summary
| Application | ELISA |
| Sample Tested | Serum |
| Species | Rat |
| Other Experimental Details | This antibody was used as the detection in a matched pair with MAB519 in the development of an ELISA targeting rat IL-10. |
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Related Product & Information
| Background | IL-10 |
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| background_content | Background: IL-10 Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2). |
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Citations
- In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006;181(1):57-67.
Species: Rat
Sample Type: Plasma
Application: ELISA Development
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