抗IL-10抗体(Anti-IL-10, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse IL‑10 (R&D Systems, Catalog # 417-ML)Ser19-Ser178Accession # NP_034678
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-10 in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant rat IL‑10 is observed, 1% cross‑reactivity with recombinant cotton rat IL-10 is observed, and less than 0.07% cross‑reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 sR, rhIL‑10 sR, recombinant canine IL-10, recombinant feline IL-10, and recombinant porcine IL-10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑10 (Catalog # 417-ML)
Mouse IL-10 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑10 Antibody (Catalog #MAB417 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑10 Biotinylated Antibody (Catalog #BAF417 )
ELISA Standard		Recombinant Mouse IL-10 Protein (Catalog #417-ML )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10	Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL-20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2 - 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70% - 77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8 - 10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12 - 14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15 - 17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

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References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
Hsu, D.-H. et al. (1990) Science 250:830.
Moore, K. et al. (1990) Science 248:1230.
Windsor, W.T. et al. (1993) Biochemistry 32:8807.
Syto, R. et al. (1998) Biochemistry 37:16943.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name:	Interleukin 10
Entrez Gene IDs:	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names:	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Related Research Areas

B Cell Development and Differentiation

B Cell Markers

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

IL-10/Interferon Family

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Lymphoma

Macrophage Activation Markers

Molecules Secreted by VSMC

Monocyte Markers

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

Neuroinflammation

Plasmacytoid Dendritic Cells

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Th2 Cells

Th22 Cells

Th9 Cells

A critical role for IRF5 in regulating allergic airway inflammation Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova Mucosal Immunol, 2017;10(3):716-726. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA detection

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Related Product & Information

Background

IL-10

background_content

Background: IL-10 Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL-20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2 - 5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70% - 77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8 - 10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12 - 14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15 - 17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

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Citations

1. A critical role for IRF5 in regulating allergic airway inflammation
   Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
   Mucosal Immunol, 2017;10(3):716-726.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA detection


2. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
   Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
   J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


3. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
   Authors: Jung-Yeon Lim
   Sci Rep, 2016;6(0):26851.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


4. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
   Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
   J Immunol, 2015;194(1):334-48.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Development


5. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


6. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
   Microbes Infect., 2009;11(4):524-30.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
   Immunology, 2008;125(2):272-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


8. Tumor-specific Th17-polarized cells eradicate large established melanoma.
   Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
   Blood, 2008;112(2):362-73.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
   Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
   Am. J. Pathol., 2006;168(6):1940-50.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


10. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development

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