抗IL-6抗体(Anti-IL-6, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse IL-6Phe25-Leu211Accession # P08505
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross‑reactivity with recombinant human IL-6, recombinant rat IL-6, and recombinant porcine IL-6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑6 (Catalog # 406-ML)
Mouse IL-6 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑6 Antibody (Catalog #MAB406 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑6 Biotinylated Antibody (Catalog #BAF406 )
ELISA Standard		Recombinant Mouse IL-6 Protein (Catalog #406-ML )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).

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References

Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
Jones, S.A. (2005) J. Immunol. 175:3468.
Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
Yatsenko, O.P. et al. (2004) Cytokine 28:190.
Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. 85:7099.
Murakami, M. et al. (1993) Science 260:1808.
Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name:	Interleukin 6
Entrez Gene IDs:	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
Alternate Names:	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Related Research Areas

Adipocytokines

Cancer Biomarkers

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

Diabetes

gamma delta T Cells

Glucose Homeostasis

Helper T (Th) Cells

HIF Transcription Factors

IL-6 Family

Infectious Diseases

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Multiple Myeloma

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Neuroinflammation

Obesity

Plasmacytoid Dendritic Cells

T Cell Cytokine Signaling

Th17 Cells

Th22 Cells

Th9 Cells

The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin J. Leukoc. Biol., 2017;0(0):. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA detection

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Related Product & Information

Background

IL-6

background_content

Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).

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Citations

1. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
   Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA detection


2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


3. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling
   Authors: S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat
   J. Biol. Chem, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
   Authors: Jung-Yeon Lim
   Sci Rep, 2016;6(0):26851.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


5. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
   Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
   J Clin Invest, 2015;125(4):1620-36.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ELISA detection


6. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
   Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
   J Immunol, 2013;190(10):5178-86.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA detection


7. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
   Authors: Meng Y, Efimova EV, Hamzeh KW
   Mol. Ther., 2012;20(5):1046-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


9. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
   Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
   Crit. Care Med., 2009;37(5):1567-73.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


10. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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