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HOME> 試薬> 遺伝子工学> エピジェネティクス> EpiQuik Nuclear/Whole Cell Extraction Kit

哺乳動物細胞、組織から核抽出物／全細胞抽出物を調製するキット EpiQuik Nuclear/Whole Cell Extraction Kit

掲載日情報：2017/03/06 現在Webページ番号：2214

エピジェンテックグループ /
Epigentek Group Inc.
[メーカー略称：EPG]

哺乳動物細胞、組織から核抽出物または全細胞抽出物を、簡便、迅速かつ高い再現性で調製するキットです。核抽出物を調製するキットには、核酸含有の核抽出物を調製するキット（Kit Ⅰ）と核酸不含有の核抽出物を調製するキット（Kit Ⅱ）があり、それぞれ目的に合わせて最適なキットをご選択いただけます。

※ 全ヒストン抽出物を調製するキットはこちらをご覧下さい。
※ 本製品は研究用です。研究用以外には使用できません。

エピジェネティクスオンラインカタログ リンク
#OP-0002-1　HDACの活性

左図：EpiQuik Nuclear Extraction Kit(#OP-0002-1)を用いてMCF-7細胞から核抽出物を調製し、様々な量の核抽出物を用いてHDACの活性を測定した。 図の結果はキットの特異性が高いことを示しています。

右図：EpiQuik Whole Cell Extraction Kit（#OP-0003-100）を用いてMCF-7細胞から全細胞抽出物を調製した。様々な量の全細胞抽出物を用いてSOD（Superoxide dismutase）活性を測定した。

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ラインナップ

商品コードをクリックすると価格表がご覧いただけます。

品名 EpiQuik Nuclear Extraction Kit EpiQuik Whole Cell
Extraction Kit
Kit Ⅰ Kit Ⅱ
商品コード OP-0002-1 OP-0022-100 OP-0003-100
使用回数 100 回
試料 哺乳動物細胞／組織
抽出物の種類 核抽出物 全細胞抽出物
核酸含有 核酸不含有
全操作時間 60分 45分
適用例 ウエスタン
ブロッティング		 ● ●
酵素アッセイ ● ●
タンパク質－DNA
結合アッセイ		 ● －
キット
内容		 Pre-extraction buffer ● ● －
Extraction buffer ● ● ●
Extraction
Pre-cleaner		 － ● －
Extraction cleaner － ● －
Protease inhibitor cocktail ● ● ●
DTT solution ● ● ●

※ キットコンポーネントは各キットによって内容が異なる場合があります。それぞれのキット専用のものをご使用下さい。

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操作方法概要

商品コードをクリックすると価格表がご覧いただけます。

品名 EpiQuik Nuclear Extraction Kit EpiQuik Whole Cell
Extraction Kit
Kit Ⅰ Kit Ⅱ
商品コード OP-0002-1 OP-0022-100 OP-0003-100
操作方法概要 細胞
哺乳動物細胞、組織
緑バイアル
前処理		 緑バイアル
細胞の溶解
回転バイアル
10分間、12,000 rpm		 回転バイアル
10 min, 14,000 rpm
緑バイアル
細胞の溶解		 赤バイアル
全細胞抽出物
回転バイアル
10分間, 14,000 rpm		 　
赤バイアル
核抽出物（核酸含有）		 緑バイアル
核酸の消化
回転バイアル
1分間, 14,000 rpm
赤バイアル
核抽出物（核酸不含有）

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使用文献

使用文献 (クリックで開閉します)
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 納期 文献数
Nuclear Extraction Kit I, EpiQuik (100assays)
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 337
説明文
哺乳動物細胞および組織から核抽出物を調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4
ニュース2021年3月15日号 p.13

製品記事 EpiQuik HDAC Activity / Inhibition Assay Kit
EpiQuik DNA Demethylase Activity / Inhibition Assay Kit
Epigenase HDAC/SIRT Activity/Inhibition Assay Kit
タンパク質抽出 - ウエスタンブロット(Western Blot)特集
ウイルスのRNA修飾および宿主由来RNAの研究用製品（EpiGentek社）
関連記事
Nuclear Extraction Kit II, for Nucleic Acid-free Extraction, EpiQuik (100assays)
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 16
説明文
哺乳動物細胞および組織から，核酸を含まない核抽出物を調製するキット。
法規制等
保存条件 4℃,-20℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4
ニュース2021年3月15日号 p.13

製品記事 タンパク質抽出 - ウエスタンブロット(Western Blot)特集
関連記事
Whole Cell Extraction Kit, EpiQuik (100assays)
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 23
説明文
哺乳動物細胞，組織から全細胞抽出物を，簡便，迅速かつ高い再現性で調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4

製品記事 タンパク質抽出 - ウエスタンブロット(Western Blot)特集
関連記事

[在庫・価格 ：2024年04月26日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Nuclear Extraction Kit I, EpiQuik (100assays)

文献数： 337

説明文 哺乳動物細胞および組織から核抽出物を調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4
ニュース2021年3月15日号 p.13

製品記事 EpiQuik HDAC Activity / Inhibition Assay Kit
EpiQuik DNA Demethylase Activity / Inhibition Assay Kit
Epigenase HDAC/SIRT Activity/Inhibition Assay Kit
タンパク質抽出 - ウエスタンブロット(Western Blot)特集
ウイルスのRNA修飾および宿主由来RNAの研究用製品（EpiGentek社）
関連記事

Nuclear Extraction Kit II, for Nucleic Acid-free Extraction, EpiQuik (100assays)

文献数： 16

説明文 哺乳動物細胞および組織から，核酸を含まない核抽出物を調製するキット。
法規制等
保存条件 4℃,-20℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4
ニュース2021年3月15日号 p.13

製品記事 タンパク質抽出 - ウエスタンブロット(Western Blot)特集
関連記事

Whole Cell Extraction Kit, EpiQuik (100assays)

文献数： 23

説明文 哺乳動物細胞，組織から全細胞抽出物を，簡便，迅速かつ高い再現性で調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2021年12月15日号 p.4

製品記事 タンパク質抽出 - ウエスタンブロット(Western Blot)特集
関連記事

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