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HOME> 試薬> 遺伝子工学> エピジェネティクス> EpiQuik Nuclear/Whole Cell Extraction Kit

哺乳動物細胞,組織から核抽出物/全細胞抽出物を調製するキット EpiQuik Nuclear/Whole Cell Extraction Kit

掲載日情報:2017/03/06 現在Webページ番号:2214

哺乳動物細胞,組織から核抽出物または全細胞抽出物を,簡便,迅速かつ高い再現性で調製するキットです。核抽出物を調製するキットには,核酸含有の核抽出物を調製するキット(Kit Ⅰ)と核酸不含有の核抽出物を調製するキット(Kit Ⅱ)があり,それぞれ目的に合わせて最適なキットをご選択いただけます。

全ヒストン抽出物を調製するキットはこちらをご覧下さい。
本製品は研究用です。研究用以外には使用できません。

エピジェネティクスオンラインカタログ リンク
#OP-0002-1 HDACの活性

左図:EpiQuik Nuclear Extraction Kit(#OP-0002-1)を用いてMCF-7細胞から核抽出物を調製し,様々な量の核抽出物を用いてHDACの活性を測定した。 図の結果はキットの特異性が高いことを示しています。

右図:EpiQuik Whole Cell Extraction Kit(#OP-0003-100)を用いてMCF-7細胞から全細胞抽出物を調製した。様々な量の全細胞抽出物を用いてSOD(Superoxide dismutase)活性を測定した。

ラインナップ

商品コードをクリックすると価格表がご覧いただけます。

品名 EpiQuik Nuclear Extraction Kit EpiQuik Whole Cell
Extraction Kit
Kit Ⅰ Kit Ⅱ
商品コード OP-0002-1 OP-0022-100 OP-0003-100
使用回数 100 回
試料 哺乳動物細胞/組織
抽出物の種類 抽出物 全細胞抽出物
核酸含有 核酸不含有
全操作時間 60 45
適用例 ウエスタン
ブロッティング
酵素アッセイ
タンパク質-DNA
結合アッセイ
キット
内容
Pre-extraction buffer
Extraction buffer
Extraction
Pre-cleaner
Extraction cleaner
Protease inhibitor cocktail
DTT solution

キットコンポーネントは各キットによって内容が異なる場合があります。それぞれのキット専用のものをご使用下さい。

目次に戻る

操作方法概要

商品コードをクリックすると価格表がご覧いただけます。

品名 EpiQuik Nuclear Extraction Kit EpiQuik Whole Cell
Extraction Kit
Kit Ⅰ Kit Ⅱ
商品コード OP-0002-1 OP-0022-100 OP-0003-100
操作方法概要 細胞
哺乳動物細胞,組織
緑バイアル
前処理
緑バイアル
細胞の溶解
回転バイアル
10分間,12,000 rpm
回転バイアル
10 min, 14,000 rpm
緑バイアル
細胞の溶解
赤バイアル
全細胞抽出物
回転バイアル
10分間, 14,000 rpm
 
赤バイアル
抽出物(核酸含有
緑バイアル
核酸の消化
回転バイアル
1分間, 14,000 rpm
赤バイアル
抽出物(核酸不含有

目次に戻る

使用文献

使用文献 (クリックで開閉します)
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[在庫・価格 :2019年07月18日 20時04分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。
詳細 商品名
  • 商品コード
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  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数 リスト
Nuclear Extraction Kit I, EpiQuik (100assays)
10日程度  174 追加
説明文
哺乳動物細胞および組織から核抽出物を調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年4月1日号 p.13

製品記事 EpiQuik HDAC Activity / Inhibition Assay Kit
EpiQuik DNA Demethylase Activity / Inhibition Assay Kit
Epigenase HDAC/SIRT Activity/Inhibition Assay Kit
ウエスタンブロット(Western Blot)特集
関連記事
Nuclear Extraction Kit II, for Nucleic Acid-free Extraction, EpiQuik (100assays)
10日程度  6 追加
説明文
哺乳動物細胞および組織から,核酸を含まない核抽出物を調製するキット。
法規制等
保存条件 4℃,-20℃ 法規備考
掲載カタログ ニュース2019年4月1日号 p.13
ニュース2018年5月1日号 p.11

製品記事 EpiQuik DNMT Activity / Inhibition Assay Ultra Kit
ウエスタンブロット(Western Blot)特集
関連記事
Whole Cell Extraction Kit, EpiQuik (100assays)
10日程度  5 追加
説明文
哺乳動物細胞,組織から全細胞抽出物を,簡便,迅速かつ高い再現性で調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 ウエスタンブロット(Western Blot)特集
関連記事

[在庫・価格 :2019年07月18日 20時04分現在]

※ 納期として表示している期間は弊社発注日からの「目安」となり、納期を保証する物ではございません。実際には長くかかる場合もございます。ご注意ください。

Nuclear Extraction Kit I, EpiQuik (100assays)

文献数:174

  • 商品コード:OP-0002-1
  • メーカー:EPG
  • 包装:1kit
  • 価格:¥34,000
  • 在庫:無(未発注)
  • 納期:10日程度 
  • 法規制等:

説明文 哺乳動物細胞および組織から核抽出物を調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年4月1日号 p.13

製品記事 EpiQuik HDAC Activity / Inhibition Assay Kit
EpiQuik DNA Demethylase Activity / Inhibition Assay Kit
Epigenase HDAC/SIRT Activity/Inhibition Assay Kit
ウエスタンブロット(Western Blot)特集
関連記事

Nuclear Extraction Kit II, for Nucleic Acid-free Extraction, EpiQuik (100assays)

文献数:6

  • 商品コード:OP-0022-100
  • メーカー:EPG
  • 包装:1kit
  • 価格:¥34,000
  • 在庫:無(未発注)
  • 納期:10日程度 
  • 法規制等:

説明文 哺乳動物細胞および組織から,核酸を含まない核抽出物を調製するキット。
法規制等
保存条件 4℃,-20℃ 法規備考
掲載カタログ ニュース2019年4月1日号 p.13
ニュース2018年5月1日号 p.11

製品記事 EpiQuik DNMT Activity / Inhibition Assay Ultra Kit
ウエスタンブロット(Western Blot)特集
関連記事

Whole Cell Extraction Kit, EpiQuik (100assays)

文献数:5

  • 商品コード:OP-0003-100
  • メーカー:EPG
  • 包装:1kit
  • 価格:¥31,000
  • 在庫:無(未発注)
  • 納期:10日程度 
  • 法規制等:

説明文 哺乳動物細胞,組織から全細胞抽出物を,簡便,迅速かつ高い再現性で調製するキット。
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 ウエスタンブロット(Western Blot)特集
関連記事

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