100th つないで、進む FRONTIERS IN LIFE SCIENCE

検索の使い方

HOME> 試薬> 細胞情報伝達> エピジェネティクス> ヒストンアセチル化／メチル化／リン酸化> EpiQuik Total Histone Extraction Kit
HOME> 試薬> タンパク質／酵素> タンパク質解析> タンパク質の抽出・精製> EpiQuik Total Histone Extraction Kit
HOME> 試薬> 遺伝子工学> エピジェネティクス> ヒストンアセチル化／メチル化／リン酸化> EpiQuik Total Histone Extraction Kit

細胞や組織から総コアヒストンタンパク質を抽出するキット EpiQuik Total Histone Extraction Kit

掲載日情報：2019/11/13 現在Webページ番号：63442

エピジェンテックグループ /
Epigentek Group Inc.
[メーカー略称：EPG]

哺乳動物細胞や組織から総コアヒストンタンパク質（H2A, H2B, H3, H4）を迅速に抽出するキットです。

※ 本製品は研究用です。研究用以外には使用できません。

カートに商品を
追加しました。

特長

  • 抽出したヒストンの翻訳後修飾は維持されるため、修飾ヒストン（メチル化／アセチル化・リン酸化、SUMO化、ユビキチン化など）の測定に使用できます。
  • キットには100回の抽出操作を行うための試薬が含まれています。
  • 1回の操作で、1×107細胞または100 mgの組織から約0.4 mgのヒストンを抽出できます*。
  • 1回当たりの試料量：1×105～1×107細胞 、1 mg～100 mg組織
  • 操作時間：60分

＊細胞や組織の種類によってヒストンの収量は変化します。

目次に戻る

カートに商品を
追加しました。

操作方法概略

EpiQuik Total Histone Extraction Kitの操作方法概略

目次に戻る

カートに商品を
追加しました。

細胞や組織からのコアヒストン抽出例

EpiQuik Total Histone Extraction Kitを用いて抽出したヒストンのH3K9アセチル化解析

本製品を用いてMCF-7細胞からヒストンを抽出し、EpiQuik Global Acetylated Histone H3K9 Quantification Kit(#P-4010)を用いてアセチル化ヒストンH3K9を定量した。

EpiQuik Total Histone Extraction Kitを用いて抽出したヒストンのSDS-PAGE像

本製品を用いたヒストン（10μg）抽出物のSDS-PAGEの結果

目次に戻る

カートに商品を
追加しました。

キット内容

  • Pre-lysis buffer
  • Lysis buffer
  • Balance buffer
  • DTT solution

目次に戻る

カートに商品を
追加しました。

価格

[在庫・価格 ：2024年04月26日 08時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
 納期 文献数
Total Histone Extraction Kit, EpiQuik (100extractions)
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 200
説明文
哺乳動物細胞および組織から，簡便にヒストンを調製するキット。
法規制等
保存条件 室温,4℃ 法規備考
掲載カタログ ニュース2021年3月15日号 p.13
ニュース2020年5月1日号 p.14

製品記事 EpiQuik Total Histone Acetylation Detection Fast Kit
EpiQuik Histone H3/H4 Modification Multiplex Assay Kit
EpiQuik Global Di-Methyl Histone Quantification Kit
関連記事

[在庫・価格 ：2024年04月26日 08時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Total Histone Extraction Kit, EpiQuik (100extractions)

文献数： 200

説明文 哺乳動物細胞および組織から，簡便にヒストンを調製するキット。
法規制等
保存条件 室温,4℃ 法規備考
掲載カタログ ニュース2021年3月15日号 p.13
ニュース2020年5月1日号 p.14

製品記事 EpiQuik Total Histone Acetylation Detection Fast Kit
EpiQuik Histone H3/H4 Modification Multiplex Assay Kit
EpiQuik Global Di-Methyl Histone Quantification Kit
関連記事



目次に戻る

カートに商品を
追加しました。

関連製品：EpiQuik Total Histone Extraction HT Kit

哺乳動物の培養細胞から総コアヒストンタンパク質（H2A, H2B, H3, H4）を、96ウェルフォーマットで迅速かつハイスループットに抽出するキットです。

  • 試料量：104 cells/well以上（推奨：3×104 cells/well）
  • 104 cellsから約400 ngのヒストンを抽出できます。*

＊細胞の種類によってヒストンの収量は変化します。

■ EpiQuik Total Histone Extraction HT Kitの価格

[在庫・価格 ：2024年04月26日 08時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
 納期 文献数
Total Histone Extraction HT Kit, EpiQuik (192extractions)
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 4
説明文
法規制等
保存条件 室温,4℃ 法規備考
掲載カタログ

製品記事
関連記事

[在庫・価格 ：2024年04月26日 08時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Total Histone Extraction HT Kit, EpiQuik (192extractions)

文献数： 4

説明文
法規制等
保存条件 室温,4℃ 法規備考
掲載カタログ

製品記事
関連記事



目次に戻る

カートに商品を
追加しました。

EpiQuik Total Histone Extraction Kitの使用文献

Kanwal R et. al. (2016). Dietary Flavones as Dual Inhibitors of DNA Methyltransferases and Histone Methyltransferases. PLoS One. 11(9):e0162956.
Xiong Guo et. al. (2016). miR-15b facilitates the progression of colorectal cancer via targeting β-catenin and Axin2 IJCEP. 9(9):8990-8996.
Dincman TA et. al. (2016). Histone deacetylase inhibition is cytotoxic to oligodendrocyte precursor cells in vitro and in vivo. Int J Dev Neurosci. 54:53-61.
Qi W et. al. (2016). Abnormal histone acetylation of CD8+ T cells in patients with severe aplastic anemia. Int J Hematol.
Xu S et. al. (2016). Tip60 HAT Action Mediates Environmental Enrichment Induced Cognitive Restoration. PLoS One. 11(7):e0159623.
Lee HO et. al. (2016). Lack of global epigenetic methylation defects in CBS deficient mice. J Inherit Metab Dis.
Guo S et. al. (2016). Characteristics and expression patterns of histone-modifying enzyme systems in the migratory locust. Insect Biochem Mol Biol. 76:18-28.
Zhao Y et. al. (2016). Dynamic Variation of Histone H3 Trimethyl Lys4 (H3K4me3) and Heterochromatin Protein 1 (HP1) with Employment Length in Nickel Smelting Workers. Biomarkers. :1-20.
Kallingappa PK et. al. (2016). Quiescence Loosens Epigenetic Constraints in Bovine Somatic Cells and Improves Their Reprogramming into Totipotency. Biol Reprod.
Hu C et. al. (2016). Frontline Science: ATF3 is responsible for the inhibition of TNF-α release and the impaired migration of acute ethanol-exposed monocytes and macrophages. J Leukoc Biol.
Vinogradova M et. al. (2016). An inhibitor of KDM5 demethylases reduces survival of drug-tolerant cancer cells. Nat Chem Biol.
Iwanowycz S et. al. (2016). Emodin inhibits breast cancer growth by blocking the tumor-promoting feedforward loop between cancer cells and macrophages. Mol Cancer Ther.
Li N et. al. (2016). Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus. Am J Hum Genet.
Ruiz-Andres O et. al. (2016). Histone lysine-crotonylation in acute kidney injury. Dis Model Mech.
Shearstone JR et. al. (2016). Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS One. 11(4):e0153767.
Muta K et. al. (2016). Curcumin ameliorates nephrosclerosis via suppression of histone acetylation independent of hypertension Nephrol Dial Transplant.
Snigdha S et. al. (2016). H3K9me3 Inhibition Improves Memory, Promotes Spine Formation, and Increases BDNF Levels in the Aged Hippocampus. J Neurosci.
Eid A et. al. (2016). Developmental lead exposure and lifespan alterations in epigenetic regulators and their correspondence to biomarkers of Alzheimer's disease Alzheimer's and Dement. :1-9.
Liu Y et. al. (2016). Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis Evid. Based Complement. Alternat. Med.. 2016:1-9.
Adamu HA et. al. (2016). Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring. Food Nutr Res. 60:30209.
Smonskey M et. al. (2016). EZH2 inhibition re-sensitizes multidrug resistant B-cell lymphomas to etoposide mediated apoptosis Oncoscience. 3(1):1-10.
Zheng YC et. al. (2016). 1,2,3-Triazole-Dithiocarbamate Hybrids, a Group of Novel Cell Active SIRT1 Inhibitors. Cell Physiol Biochem. 38(1):185-93.
Sant KE et. al. (2016). Mono-2-ethylhexyl phthalate (MEHP) alters histiotrophic nutrition pathways and epigenetic processes in the developing conceptus. J Nutr Biochem. 27:211-8.
Burchfield JS et. al. (2016). Pharmacological priming of adipose-derived stem cells promotes myocardial repair. J Investig Med. 64(1):50-62.
Wu F et. al. (2015). 3-(Piperidin-4-ylmethoxy)pyridine Containing Compounds Are Potent Inhibitors of Lysine Specific Demethylase 1. J Med Chem.
Zeng Q et. al. (2015). Apoptosis in human myelodysplastic syndrome CD34+ cells is modulated by the upregulation of TLRs and histone H4 acetylation via a β-arrestin 1 dependent mechanism Exp Cell Res.
Inagaki Y et. al. (2015). Epigenetic regulation of proliferation and invasion in hepatocellular carcinoma cells by CBP/p300 histone acetyltransferase activity. Int J Oncol.
Huynh et. al. (2015). Inhibition of Histone Deacetylases Enhances the Osteogenic Differentiation of Human Periodontal Ligament Cells J Cell Biol.
Han Y et. al. (2015). Novel Tranylcypromine/Hydroxylcinnamic Acid Hybrids as Lysine-Specific Demethylase 1 Inhibitors with Potent Antitumor Activity. Chem Pharm Bull (Tokyo). 63(11):882-9.
Ma L et. al. (2015). Histone Methylation in Nickel-Smelting Industrial Workers. PLoS One. 10(10):e0140339.
Sancak D et. al. (2015). Global histone modifications in Fumonisin B1 exposure in rat kidney epithelial cells. Toxicol In Vitro. 29(7):1809-15.
Keenan MM et. al. (2015). ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate. PLoS Genet. 11(10):e1005599.
Li MF et. al. (2015). High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res.
Zhuo X et. al. (2015). Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis. J Cell Biol. 210(5):727-35.
Finegersh A et. al. (2015). Repeated vapor ethanol exposure induces transient histone modifications in the brain that are modified by genotype and brain region Front Mol Neurosci. 8:39.
Plagg B et. al. (2015). Increased Acetylation of Histone H4 at Lysine 12 (H4K12) in Monocytes of Transgenic Alzheimer's Mice and in Human Patients. Curr Alzheimer Res. 12(8):752-60.
Yan N et. al. (2015). Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease. Mol Cell Endocrinol.
Kim KP et. al. (2015). First-in-human study of the toxicity, pharmacokinetics, and pharmacodynamics of CG200745, a pan-HDAC inhibitor, in patients with refractory solid malignancies. Invest New Drugs.
Puig-Vilanova E et. al. (2015). Quadriceps muscle weakness and atrophy are associated with a differential epigenetic profile in advanced COPD. Clin Sci (Lond). 128(12):905-21.
Wang J et. al. (2015). Cadmium induced apoptosis in mouse primary hepatocytes: the role of oxidative stress-mediated ERK pathway activation and the involvement of histone H3 phosphorylation RSC Advances. 5:31798-31806.
Tu P et. al. (2015). Liver histone H3 methylation and acetylation associate with type 2 diabetes development. J Physiol Biochem. 71(1):89-98.
Ma LY et. al. (2015). Design, Synthesis, and Structure-Activity Relationship of Novel LSD1 Inhibitors Based on Pyrimidine-Thiourea Hybrids As Potent, Orally Active Antitumor Agents. J Med Chem. 58(4):1705-16.
Kirk JS et. al. (2015). Top2a identifies and provides epigenetic rationale for novel combination therapeutic strategies for aggressive prostate cancer. Oncotarget. 6(5):3136-46.
Li C et. al. (2015). DNA methylation and histone modification patterns during the late embryonic and early postnatal development of chickens. Poult Sci.
Geis-Asteggiante L et. al. (2015). Top-down analysis of low mass proteins in exosomes shed by murine myeloid-derived suppressor cells. Int J Mass Spectrom. 378:264-269.
Pacaud R et. al. (2015). Histone H3 Phosphorylation in GBM: a New Rational to Guide the Use of Kinase Inhibitors in anti-GBM Therapy. Theranostics. 5(1):12-22.
Rouleau SG et. al. (2015). Small antisense oligonucleotides against G-quadruplexes: specific mRNA translational switches. Nucleic Acids Res. 43(1):595-606.
Zhu G et. al. (2014). Elevated expression of histone demethylase PHF8 associates with adverse prognosis in patients of laryngeal and hypopharyngeal squamous cell carcinoma. Epigenomics. :1-11.
Burke MC et. al. (2014). Ubiquitinated proteins in exosomes secreted by myeloid-derived suppressor cells. J Proteome Res. 13(12):5965-72.
Zhang L et. al. (2014). Inhibition of histone H3K79 methylation selectively inhibits proliferation, self-renewal and metastatic potential of breast cancer. Oncotarget. 5(21):10665-77.
Li C et. al. (2014). Maternal high-zinc diet attenuates intestinal inflammation by reducing DNA methylation and elevating H3K9 acetylation in the A20 promoter of offspring chicks. J Nutr Biochem.
Dong G et. al. (2014). Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows. PLoS One. 9(9):e107659.
Schulz MD et. al. (2014). High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity. Nature.
Geis-Asteggiante L et. al. (2014). Top–down analysis of low mass proteins in exosomes shed by murine myeloid-derived suppressor cells International Journal of Mass Spectrometry. Abstract
Kumar P et. al. (2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.
Dong Y et. al. (2014). H2AX phosphorylation regulated by p38 is involved in Bim expression and apoptosis in chronic myelogenous leukemia cells induced by imatinib. Apoptosis.
Elhammali A et. al. (2014). A High-Throughput Fluorimetric Assay for 2-Hydroxyglutarate Identifies Zaprinast as a Glutaminase Inhibitor. Cancer Discov.
Kraus D et. al. (2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. 508(7495):258-62.
Siuda D et. al. (2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.
Tafrova JI et. al. (2014). Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo. Mol Cell Biochem.
Kumar P et. al. (2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.
Liu D et. al. (2014). Histone acetylation and expression of mono-aminergic transmitters synthetases involved in CUS-induced depressive rats. Exp Biol Med (Maywood). 239(3):330-6.
Monteiro JB et. al. (2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.
Jochems J et. al. (2014). Antidepressant-like properties of novel HDAC6-selective inhibitors with improved brain bioavailability. Neuropsychopharmacology. 39(2):389-400.
Zheng YC et. al. (2013). Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration. J Med Chem. 56(21):8543-60.
Aguilar-Valles A et. al. (2013). Methamphetamine-Associated Memory Is Regulated by a Writer and an Eraser of Permissive Histone Methylation. Biol Psychiatry.
Rossi D et. al. (2013). Croton lechleri Müll. Arg. (Euphorbiaceae) stem bark essential oil as possible mutagen-protective food ingredient against heterocyclic amines from cooked food. Food Chem. 139(1-4):439-47.
Navrkalova V et. al. (2013). The p53 pathway induction is not primarily dependent on Ataxia Telangiectasia Mutated (ATM) gene activity after fludarabine treatment in chronic lymphocytic leukemia cells. Leuk Lymphoma. 54(8):1840-3.
Shyamasundar S et. al. (2013). Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One. 8(6):e65945.
Itzhak Y et. al. (2013). Sodium butyrate-induced histone acetylation strengthens the expression of cocaine-associated contextual memory. Neurobiol Learn Mem. 102:34-42.
Deng L et. al. (2013). Synthesis, Activity and Metabolic Stability of Non-Ribose Containing Inhibitors of Histone Methyltransferase DOT1L. Medchemcomm. 4(5):822-826.
Luo S et. al. (2013). Aberrant histone modifications in peripheral blood mononuclear cells from patients with Henoch-Schönlein purpura. Clin Immunol. 146(3):165-75.
Meng F et. al. (2013). Anticancer efficacy of cisplatin and trichostatin A or 5-aza-2'-deoxycytidine on ovarian cancer. Br J Cancer. 108(3):579-86.
Timofeeva OA et. al. (2013). STAT3 suppresses transcription of proapoptotic genes in cancer cells with the involvement of its N-terminal domain. Proc Natl Acad Sci U S A. 110(4):1267-72.
Kao GS et. al. (2012). Neonatal isolation decreases cued fear conditioning and frontal cortical histone 3 lysine 9 methylation in adult female rats. Eur J Pharmacol. 697(1-3):65-72.
McCabe MT et. al. (2012). EZH2 inhibition as a therapeutic strategy for lymphoma with EZH2-activating mutations. Nature. 492(7427):108-12.
Fan H et. al. (2012). Sulforaphane causes a major epigenetic repression of myostatin in porcine satellite cells. Epigenetics. 7(12):1379-90.
Rehan VK et. al. (2012). Perinatal nicotine exposure induces asthma in second generation offspring. BMC Med. 10:129.
Yamamura Y et. al. (2012). TNF-α inhibits aquaporin 5 expression in human salivary gland acinar cells via suppression of histone H4 acetylation. J Cell Mol Med. 16(8):1766-75.
Qu F et. al. (2012). A molecular mechanism underlying ovarian dysfunction of polycystic ovary syndrome: hyperandrogenism induces epigenetic alterations in the granulosa cells. J Mol Med (Berl). 90(8):911-23.
Wurm T et. al. (2012). The HTLV-1-encoded protein HBZ directly inhibits the acetyl transferase activity of p300/CBP. Nucleic Acids Res. 40(13):5910-25.
Itzhak Y et. al. (2012). Histone acetylation rescues contextual fear conditioning in nNOS KO mice and accelerates extinction of cued fear conditioning in wild type mice. Neurobiol Learn Mem. 97(4):409-17.
Zhao M et. al. (2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.
Ponomarev I et. al. (2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. J Neurosci. 32(5):1884-97.
Neri F et. al. (2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51.
Jing L et. al. (2011). Effect of the histone deacetylase inhibitors on behavioural sensitization to a single morphine exposure in mice. Neurosci Lett. 494(2):169-73.
Kawakami K et. al. (2011). Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsatellite stable and CpG island methylator phenotype-negative colorectal cancer. Cancer Sci. 102(1):166-74.
Hansen A et. al. (2010). Development of a drug screening platform based on engineered heart tissue. Circ Res. 107(1):35-44.
Diehl F et. al. (2010). Cardiac deletion of Smyd2 is dispensable for mouse heart development. PLoS One. 5(3):e9748.

目次に戻る

カートに商品を
追加しました。

お問い合わせ先

（テクニカルサポート 試薬担当）

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。

エピジェネティクス