抗PDI [RL90]抗体 | Anti-PDI [RL90] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90]. Red : Primary antibodyGreen : ActinFixation : FormalinPermeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperatureDilution : 1:75 for at least 1 hour at room temperature
	IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	FACS analysis of NIH/3T3 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).Dilution : 0.5 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde
	WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90]. Dilution : 1:1000
	FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).Dilution : 1 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde
	IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibodyBlue : Nuclei
	ICC/IF analysis of A549 cells using GTX22792 PDI antibody [RL90]. Green : Primary antibodyBlue : Nuclei
	ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90]. Permeabilization : 0.1% Triton X-100Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature
	ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibodyBlue : Nuclei
	ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90]. Fixation : FormalinPermeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperatureDilution : 1:75 for at least 1 hour at room temperature
	ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibodyBlue : Nuclei
	IHC-P analysis of human tonsil tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:200
	FACS analysis of K562 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).Dilution : 1 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	RL90
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IM, Neutralizing/Blocking
Reactivity	Human, Mouse, Rat, Dog, Hamster, Pig, Rhesus Monkey, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:200-1:2000
ICC/IF	1:100
IHC-P	1:100
IHC-Fr	1:100
FACS	0.5-1 μg/test
IP	Assay dependent
IM	Assay dependent
Neutralizing/Blocking	Assay dependent

Not tested in other applications.

Calculated MW	57 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Buffer, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Rat
Immunogen	Purified rat PDI protein.
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	prolyl 4-hydroxylase subunit beta , PDI , PDIR
Cellular Localization	Endoplasmic reticulum,Cell membrane
Background	may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]
Database	・ Gene ID: 25506 P4hb ・ UniProt: P04785 P4hb

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Yating Cheng et al. Pakistan Journal of Zoology 2019; (Epub) http://researcherslinks.com/current-issues/New-Strategy-for-Expression-of-Recombinant/20/1/2539/html.

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