Human IL-1 beta/IL-1F2 Quantikine ELISA Kit | hIL-1b QKit
掲載日情報：2016/08/18 現在Webページ番号：111132
R&D Systems製のHuman IL-1 beta/IL-1F2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月06日 15時55分現在]
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IL-1β, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年05月06日 15時55分現在]
IL-1β, Human, ELISA Kit, Quantikine (96well)
文献数： 326
- 商品コード：DLB50
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：3.9-250 pg/mL，感度：1 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC01-1，測定時間：3.5 hours or 4.5 hours，測定因子：IL-1 beta, IL-1F2 別名：catabolin Genbank No： 3553 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | 新型コロナウイルス感染に起因するサイトカインストームのモニタリング用製品 Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours or 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
- Sensitivity
- 1 pg/mL
- Assay Range
- 3.9 - 250 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-1 beta
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
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Precision
Intra-Assay Precision (Precision within an assay)
Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | ||||||
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 18.9 | 30.2 | 122 | 181 | 29.8 | 118 | 193 |
| Standard Deviation | 1.6 | 1 | 5.4 | 5 | 2.5 | 5 | 8 |
| CV% | 8.5 | 3.3 | 4.4 | 2.8 | 8.4 | 4.2 | 4.1 |
| Intra-Assay Precision | Inter-Assay Precision | ||||||
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 12.6 | 30.4 | 69.2 | 192 | 30.8 | 120 | 194 |
| Standard Deviation | 0.3 | 1 | 1.6 | 6.5 | 2.2 | 4.1 | 7.9 |
| CV% | 2.4 | 3.3 | 2.3 | 3.4 | 7.1 | 3.4 | 4.1 |
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Recovery
The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=10) | 95 | 87-110 |
| Heparin Plasma (n=10) | 82 | 76-100 |
| Citrate Plasma (n=10) | 93 | 83-110 |
| EDTA Plasma (n=10) | 86 | 81-100 |
| Cell Culture Media (n=4) | 97 | 80-111 |
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Linearity
To assess linearity of the assay, the following biological samples containing, or spiked with, high concentrations of IL-1 beta were diluted with the appropriate Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-1 beta/IL-1F2
- Aliases
- IL1B; IL-1F2
- Entrez Gene IDs
- 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 100034237 (Equine); 100135556 (Guinea Pig)
- Background
- IL-1 beta/IL-1F2
- IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
Chen N, Chen S, Hsieh S, Li L, Lin W, Lin Y, Tsai I, Wu M, Yang A
Blood 2013 121:95-106
Species: Human
Sample Type: Cell Culture Supernates
Validation and quality control of ELISAs for the use with human saliva samples.
Jaedicke KM, Preshaw PM, Taylor JJ
J. Immunol. Methods 2012 377:62-5
The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
Huang T, Ko Y, Lai H, Lu C, Ojcius D, Wong T, Wu C, Wu Y, Young J
PLoS ONE 2012 7:e41383
Species: Human
Sample Type: Cell Culture Supernates
A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
Acta Pharmacol. Sin. 2012 33:1204-16
IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease.
Bonagura VR, Goldofsky E, Pathak S, Vambutas A, Vivas EX
J. Immunol. 2011 186:1870-9
Species: Human
Sample Type: Plasma
High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Deng M, Ding B, Jin Y, Liu L, Liu N, Liu W, Liu Y, Shi H, Shi S, Tang L, Zhang G
J. Bone Miner. Res. 2011 26:2082-95
Species: Human
Sample Type: Cell Culture Supernates
Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
Bracke KR, Brusselle GG, Dupont LL, Joos GF, Lambrecht BN, Pauwels NS, Provoost S, Van Pottelberge GR, Vanden Berghe T, Vandenabeele P
Eur. Respir. J. 2011 38:1019-28
Species: Human
Sample Type: Sputum
Bacillus anthracis capsule activates caspase-1 and induces interleukin-1beta release from differentiated THP-1 and human monocyte-derived dendritic cells.
Ahn HJ, Cho MH, Chun JH, Ha HJ, Kim BS, Oh HB, Park J, Rhie GE
Infect. Immun. 2010 78:387-92
Species: Human
Sample Type: Cell Culture Supernates
The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
Berthier CC, Kaplan MJ, Kretzler M, Mattinzoli D, Rastaldi MP, Thacker SG
J. Immunol. 2010 185:4457-69
Species: Human
Sample Type: Serum
Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
Bachert C, Bousquet J, De Lobel L, Holtappels G, Lin P, Liu S, Van Cauwenberge P, Van Steen K, Zhang N
J. Allergy Clin. Immunol. 2010 126:962
Species: Human
Sample Type: Tissue Homogenates
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