Human TIM-1/KIM-1/HAVCR Quantikine ELISA Kit | hTIM-1/KIM-1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111128
R&D Systems製のHuman TIM-1/KIM-1/HAVCRを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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TIM-1, Human, ELISA Kit, Quantikine(96well) <KIM-1, ELISA Kit><HAVCR, ELISA Kit> |
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[在庫・価格 ：2025年04月27日 00時00分現在]
TIM-1, Human, ELISA Kit, Quantikine(96well) <KIM-1, ELISA Kit><HAVCR, ELISA Kit>
文献数： 25
- 商品コード：DKM100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.156-10 ng/mL，感度：0.046 ng/mL，測定試料：Urine，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC24，測定時間：4.5 hours，測定因子：TIM-1, KIM-1, HAVCR 別名：CD365 Genbank No： 26762 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2022年11月15日号 p.6
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| 製品記事 | Human TIM-1/KIM-1/HAVCR Quantikine® ELISA Kit Quantikine TIM-1 ELISA Kit Immune Checkpoint Protein ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Urine (50 µL)
- Sensitivity
- 0.046 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Urine)
- Specificity
- Natural and recombinant human TIM-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human TIM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIM-1 in urine. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIM-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.98 | 3.04 | 5.88 | 1.09 | 3.19 | 6.23 |
| Standard Deviation | 0.042 | 0.119 | 0.259 | 0.069 | 0.193 | 0.484 |
| CV% | 4.3 | 3.9 | 4.4 | 6.3 | 6 | 7.8 |
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Recovery
The recovery of TIM-1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Urine (n=20) | 104 | 94-112 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. For more information on linearity and hand
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Additional Infomation
- Molecule Information
- TIM-1/KIM-1/HAVCR
- Aliases
- HAVCR1; KIM-1
- Entrez Gene IDs
- 26762 (Human); 171283 (Mouse); 286934 (Rat)
- Background
- TIM-1/KIM-1/HAVCR
- TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2 cell mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain. The ECD contains one V-type Ig like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation.The TIM-1 gene is highly polymorphic and undergoes alternate splicing. For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A. There are two cytoplasmic alternate splice forms of TIM-1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in TIM-1a. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule. The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin. TIM1 ligation induces T cell proliferation and promotes cytokine production.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
de Borst MH, Gansevoort RT, Laverman GD, Nauta FL, Navis G, Vogt L
PLoS ONE 2012 7:e37957
Species: Human
Sample Type: Urine
Measuring urinary tubular biomarkers in type 2 diabetes does not add prognostic value beyond established risk factors.
Kidney Int 2012 82:812-8
Urinary N-acetyl-beta-D glucosaminidase as a surrogate marker for renal function in autosomal dominant polycystic kidney disease: 1 year prospective cohort study.
BMC Nephrol 2012 13:93
Species: Human
Sample Type: Urine
Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury.
Brand K, Haubitz M, Herget-Rosenthal S, Kirsch T, Mentes E, Metzger J, Mischak H, Schiffer E, Ulger P, Weissinger EM
Kidney Int. 2010 78:1252-62
Species: Human
Sample Type: Urine
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