Human IL-5 Quantikine ELISA Kit | hIL-5 QKit
掲載日情報：2016/08/18 現在Webページ番号：111002
R&D Systems製のHuman IL-5を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 19時55分現在]
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IL-5, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月06日 19時55分現在]
IL-5, Human, ELISA Kit, Quantikine (96well)
文献数： 42
- 商品コード：D5000B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：カルタヘナ該当品
| 説明文 |
測定範囲：3.90 - 250 pg/mL，感度：1.08 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：IL-5 別名：BCDF mu Genbank No： 3567 |
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| 法規制等 | カルタヘナ該当品 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Anti-human TTK Affinity Purified Polyclonal Antibody CellXVivo Human Th2 Cell Differentiation Kit Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 1.08 pg/mL
- Assay Range
- 3.9 - 250 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IL-5
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-5 levels in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-5 and antibodies raised against the recombinant factor and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-5.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 31.3 | 95.6 | 173 | 32.9 | 97.9 | 180 |
| Standard Deviation | 1.4 | 3.5 | 6.5 | 4 | 6.7 | 8.4 |
| CV% | 4.5 | 3.7 | 3.8 | 12.2 | 6.8 | 4.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 28.1 | 89.3 | 170 | 31.2 | 92.6 | 174 |
| Standard Deviation | 0.89 | 2 | 7.27 | 2.5 | 4.2 | 8.7 |
| CV% | 3.2 | 2.2 | 4.2 | 8 | 4.5 | 5 |
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Recovery
The recovery of human IL-5 spiked to different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 101 | 95-107 |
| Serum (n=4) | 104 | 88-116 |
| EDTA Plasma (n=4) | 104 | 86-118 |
| Heparin Plasma (n=4) | 101 | 87-112 |
| Urine (n=4) | 96 | 91-106 |
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Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-5
- Aliases
- BCDF mu; BCGFII; EDF; Eo-CSF; TRF
- Entrez Gene IDs
- 3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
- Background
- IL-5
- Interleukin 5 (IL-5) is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: Tcell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor µ (BCDF µ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
Bachert C, Bousquet J, De Lobel L, Holtappels G, Lin P, Liu S, Van Cauwenberge P, Van Steen K, Zhang N
J. Allergy Clin. Immunol. 2010 126:962
Species: Human
Sample Type: Tissue Homogenates
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Hirata S, Ishihara O, Liu TY, Matsushita S, Narita Y, Ohyama H, Suzuki M, Uemura Y
Eur. J. Immunol. 2008 38:1012-23
Species: Human
Sample Type: Cell Culture Supernates
Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
Borrebaeck CA, Carlsson A, Ellmark P, Engstrom G, Harmenberg U, Ingvarsson J, Krogh M, Peterson C, Wahren B, Wingren C
Proteomics 2008 8:2211-9
Species: Human
Sample Type: Serum
Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
Deutschle T, Kirsche H, Niederfuhr A, Poppert S, Riechelmann H, Wellinghausen N
Allergy 2008 63:1359-67
Species: Human
Sample Type: nasal lavage
Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
Amakawa R, Amuro H, Fukuhara S, Ito T, Liu YJ, Son Y, Sugimoto H, Tajima K, Tanijiri T, Torii Y, Yokoi T
Int. Immunol. 2008 20:1321-9
Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
Chaudhuri R, Donnelly I, Hothersall EJ, Lafferty J, McInnes I, McMahon AD, McSharry C, Meiklejohn J, Sattar N, Thomson NC, Weir CJ, Wood S
Thorax 2008 63:1070-5
Species: Human
Sample Type: Serum
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Broide DH, Cho JY, Friedman PJ, Miller M, Ramsdell J, Renvall M
J. Allergy Clin. Immunol. 2007 120:1118-25
Species: Human
Sample Type: BALF
Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
Emad A, Emad Y
J. Clin. Immunol. 2007 27:605-12
Species: Human
Sample Type: BALF
Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Chiang BL, Liang YC, Lin YL
J. Leukoc. Biol. 2007 82:1473-80
Species: Human
Sample Type: Cell Culture Supernates
1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
Jawahar MS, Narayanan PR, Selvaraj P, Vidyarani M
Cytokine 2007 40:128-34
Species: Human
Sample Type: Cell Culture Supernates
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