Human IL-2 Quantikine ELISA Kit | "hIL-2 QKit, 2nd Gen"
掲載日情報：2016/08/18 現在Webページ番号：110995
R&D Systems製のHuman IL-2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月18日 12時15分現在]
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IL-2, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年04月18日 12時15分現在]
IL-2, Human, ELISA Kit, Quantikine (96well)
文献数： 136
- 商品コード：D2050
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：7 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC01-1，測定時間：4.5 hours，測定因子：IL-2 別名：aldesleukin Genbank No： 3558 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
- Sensitivity
- 7 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-2
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 98 | 619 | 1,183 | 250 | 755 | 1,470 |
| Standard Deviation | 4.2 | 18 | 24 | 12.6 | 27.8 | 74.1 |
| CV% | 4.3 | 2.9 | 2 | 5 | 3.7 | 5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 67.5 | 438 | 851 | 232 | 620 | 1,148 |
| Standard Deviation | 2.9 | 14.4 | 26.9 | 23.2 | 34.4 | 45.5 |
| CV% | 4.3 | 3.3 | 3.2 | 10 | 5.5 | 4 |
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Recovery
The recovery of human IL-2 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 99 | 89-114 |
| Heparin Plasma (n=5) | 109 | 94-129 |
| Citrate Plasma (n=5) | 105 | 98-111 |
| EDTA Plasma (n=5) | 118 | 104-139 |
| Cell Culture Media (n=5) | 101 | 96-107 |
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Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-2 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-2
- Aliases
- undefined
- Entrez Gene IDs
- 3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
- Background
- IL-2
- Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64% aa identity to mouse, rat, pig, cat, and cow IL-2, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
Buch MH, Church LD, Churchman SM, Emery P, Geiler J, Horner EA, McDermott MF, Parmar R, Ponchel F
Clin. Exp. Rheumatol. 2012 30:534-42
Species: Human
Sample Type: Serum
Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
Basta S, Hurlbut DJ, Justinich CJ, Mak N, Mulder DJ, Pooni A
Am. J. Pathol. 2011 178:744-53
Species: Human
Sample Type: Cell Culture Supernates
HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
Anderson KC, Chauhan D, Exley MA, Hideshima T, Munshi NC, Nanjappa P, Song W, Tai YT, Tian Z
Leukemia 2011 25:161-8
Species: Human
Sample Type: Cell Culture Supernates
Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
Fu F, Gu W, Shen B, Shen Z, Sun J, Wang H, Yan R, Zhang G, Zhang X, Zhou Y
PLoS ONE 2011 6:e24751
Species: Human
Sample Type: Cell Culture Supernates
Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Daniel V, Lahdou I, Naujokat C, Opelz G, Sadeghi M, Schnitzler P, Zeier M
Transplantation 2009 88:1109-16
Species: Human
Sample Type: Urine
Immunologic markers as predictors of tuberculosis-associated immune reconstitution inflammatory syndrome in HIV and tuberculosis coinfected persons in Thailand.
Ananworanich J, Apateerapong W, Avihingsanon A, Boonchokchai B, Hammer SM, Klongugkara S, Manosuthi W, Siangphoe U, Sirivichayakul S, Tieu HV
AIDS Res. Hum. Retroviruses 2009 25:1083-9
Species: Human
Sample Type: Serum
Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer.
Guo Y, Han X, Lin D, Lu N, Shi Y, Sun T, Tan W, Xue L, Zhang B, Zhang X, Zhao D, Zhou Y
Carcinogenesis 2007 28:1067-73
Species: Human
Sample Type: Cell Culture Supernates
Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
Daniel V, Huth-Kuhne A, Naujokat C, Opelz G, Sadeghi M, Susal C, Zimmermann R
AIDS Res. Hum. Retroviruses 2007 23:886-90
Species: Human
Sample Type: Plasma
CD200 is induced by ERK and is a potential therapeutic target in melanoma.
Bear JE, Buehler C, Groben P, Kim WY, McKinnon K, Penland S, Petermann KB, Rozenberg GI, Serody JS, Sharpless NE, Shields JM, Thomas NE, Zedek D
J. Clin. Invest. 2007 117:3922-3929
Species: Human
Sample Type: Cell Culture Supernates
1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
Jawahar MS, Narayanan PR, Selvaraj P, Vidyarani M
Cytokine 2007 40:128-34
Species: Human
Sample Type: Cell Culture Supernates
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