4 unique monoclonal antibodies for cancer-related laminin chains Anti-laminin mouse monoclonal antibodies | Funakoshi
Date:February 01 2019Web Page No:81185
Funakoshi Co.,Ltd.
Useful monoclonal antibodies for the research of cancer pathology
4 unique monoclonal antibodies against cancer-related laminin chains
Laminins are major extracellular matrix proteins containing over 15 members. Laminins are large hetero-trimers consisting of 5 alpha chains, 3 beta chains, and 3 gamma chains. Due to its structural varieties, it has been challenging to detect each laminin isoform by conventional antibodies.
Funakoshi commercializes 4 unique antibodies, originally developed by Dr. Kaoru Miyazaki, Professor Emeritus of Yokohama City University in Japan.
Our antibodies can separately recognize specific isoforms or structures of laminin.
![Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 and P2H).](/domestic/f-img/ds/FNA_81185_1.png)
Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 and P2H).
Features
BG5/F7 : For laminin alpha-3 chains
- Laminin alpha 3 chain, a major component of Laminin-332, has two splicing variants, alpha 3A and alpha 3B
- Clone BG5 is specific antibody for alpha 3A
- Clone F7 is the world first commercially available antibody for alpha 3B
P2H : For laminin gamma-2 chain
- Laminin gamma2, an only component of Laminin-332, is a marker gene of invasive cancer tissue
- Laminin gamma-2 chain is activated via proteolytical cleavage at N-terminal
- N-terminal proteolytic fragment of gamma-2, called γ2pf, is highly accumulated into cytosol under malignant cancer
- Clone P2H is the world first antibody for γ2pf
12D : For laminin-511
- There are two similar isoforms, laminin-511 and laminin-521 and it is difficult for conventional antibodies such as anti-alpha 5 chain to separate them.
- Clone 12D specifically react with trimeric structure of laminin-511, not react with each components alpha 5, beta 1, gamma 1 and similar isoform laminin-511.
Note
Laminin 511 is used as a culture substrate for ES and iPS cells. (See our iMatrix-511 / Easy iMatrix-511).
![Fig.2 Structure of Laminin-511](/domestic/f-img/ds/FNA_81185_2.png)
Fig.2 Structure of Laminin-511
clone | BG5 | F7 | P2H | 12D |
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Target | Laminin-α3A chain | Laminin-α3B chain | Laminin-γ2 chain N-terminal region | Laminin-511 trimer |
Normal Tissue | ![]() |
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Epitope | ![]() |
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Cancer Tissue | ![]() |
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Outline of four clones
Description | Anti-Laminin alpha 3A | Anti-Laminin alpha 3B | Anti-Laminin gamma 2 N-terminal Fragment | Anti-Laminin-511 |
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Clone | BG5 | F7 | P2H | 12D |
Specificity | Laminin alpha 3A*1 | Laminin alpha 3B | Laminin gamma 2 Domain V-NE2 | Laminin-511 |
Host and Type | Mouse Monoclonal | |||
Subclass | IgG2a | IgG1 | IgG2a | IgG2b |
Reactivity | Human | Human | Human and Mouse | Human |
Form | Mouse Ascites (Unpurifed) | |||
Application | ELISA, IHC, IP, WB | IHC (frozen & paraffin sections), WB | IHC (frozen), IP, WB (Non-reducing) *2 | |
Code | FDV-0024 | FDV-0023 | FDV-0025 | FDV-0026 |
Size | 100 uL | |||
Storage | -20 ℃ *3 |
*1 Clone BG5 only detects alpha 3A in IHC. However, this clone may react with alpha 3B in ELISA, IP and Western Blot.
*2 Clone 12D can be only used for IHC (Frozen) and under non-reducing condition for Western Blot.
*3 Non-preservative added. So these 4 clones can be degraded at 4C. We recommend -20C storage.
Data Examples
![Fig.3 IHC image of skin cancer tissue in clone BG5 and F7](/domestic/f-img/ds/FNA_81185_3.png)
Fig.3 IHC image of skin cancer tissue in clone BG5 and F7
Left : Clone BG5, Right : Clone F7
Both clones detected basal membrane like structure around the tumor but F7 clone also detected vascular basement membrane (arrow heads).
![Fig.4 IHC image of tissues in clone P2H](/domestic/f-img/ds/FNA_81185_4.png)
Fig.4 IHC image of tissues in clone P2H
Upper images : Normal mammary gland
Lower images : Breast cancer
P2H stains basement membranes surrouding mammary glands in normal tisue, whereas in a breast cancer tissue cytoplasmic accumulation of γ2pf orits fragments is detected.
![Fig.5 IHC image of breast cancer tissue in clone 12D](/domestic/f-img/ds/FNA_81185_5.png)
Fig.5 IHC image of breast cancer tissue in clone 12D
A : Vascular basement membrane
B : Mammary gland basement membrane
C : Basement membrane-like structures surrounding tumor cells
![Fig.6 Specificity of clone 12D against laminin isoforms](/domestic/f-img/ds/FNA_81185_6.png)
Fig.6 Specificity of clone 12D against laminin isoforms
Seven recombinant human laminins were coated on a multi-well plate and detected by clone 12D. The data shows clone 12D specifically reacts with Laminin 511.
Price
[Date : July 27 2024 00:10]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Anti-Laminin alpha 3B, Human, Mouse-Mono (F7) DatasheetThis may not be the latest data sheet. |
FDV-0023 | FNAFunakoshi Co.,Ltd. | 100 µl | $400 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Anti-Laminin alpha 3A, Human, Mouse-Mono (BG5) DatasheetThis may not be the latest data sheet. |
FDV-0024 | FNAFunakoshi Co.,Ltd. | 100 µl | $400 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Anti-Laminin gamma 2 N-terminal fragment, Human, Mouse-Mono (P2H) DatasheetThis may not be the latest data sheet. |
FDV-0025 | FNAFunakoshi Co.,Ltd. | 100 µl | $400 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Anti-Laminin 511, Human, Mouse-Mono (12D) DatasheetThis may not be the latest data sheet. |
FDV-0026 | FNAFunakoshi Co.,Ltd. | 100 µl | $400 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[Date : July 27 2024 00:10]
Anti-Laminin alpha 3B, Human, Mouse-Mono (F7)
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0023
- Supplier: FNA
- Size: 100µl
- Price: $400
Description | |||
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Storage | -80°C | ||
Antigen Species | Host Species | Mouse | |
Class | IgG | Label | Unlabeled |
Cross Reactivity | Human | Application | ELISA,IHC,IP,Western Blot |
Clonality | Format | ||
Purification | Acsites | Absorption | |
Link |
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Anti-Laminin alpha 3A, Human, Mouse-Mono (BG5)
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0024
- Supplier: FNA
- Size: 100µl
- Price: $400
Description | |||
---|---|---|---|
Storage | -20°C | ||
Antigen Species | Host Species | Mouse | |
Class | IgG | Label | Unlabeled |
Cross Reactivity | Human | Application | ELISA,IHC,IP,Western Blot |
Clonality | Format | ||
Purification | Acsites | Absorption | |
Link |
|
Anti-Laminin gamma 2 N-terminal fragment, Human, Mouse-Mono (P2H)
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0025
- Supplier: FNA
- Size: 100µl
- Price: $400
Description | |||
---|---|---|---|
Storage | -20°C | ||
Antigen Species | Host Species | Mouse | |
Class | IgG | Label | Unlabeled |
Cross Reactivity | Human/Mouse | Application | IHC,Western Blot |
Clonality | Format | ||
Purification | Acsites | Absorption | |
Link |
|
Anti-Laminin 511, Human, Mouse-Mono (12D)
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0026
- Supplier: FNA
- Size: 100µl
- Price: $400
Description | |||
---|---|---|---|
Storage | -80°C | ||
Antigen Species | Host Species | Mouse | |
Class | IgG | Label | Unlabeled |
Cross Reactivity | Human | Application | IHC(frozen),IP,Western Blot |
Clonality | Format | ||
Purification | Acsites | Absorption | |
Link |
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Citations and References
- Kariya, Y., et al., J. Mol. Histol., 39, 435-446 (2008) (Clone BG5/F7)
- Mori, T., et al., Cancer Sci., 102, 1095-1100 (2011) (Clone F7)
- Miyazaki, K., et al., Cancer Sci., 107, 1909-1918 (2016) (Clone BG5/P2H)
- Komiya, E., et al., Cancer Med., 3, 537-549 (2014) (Clone 12D)
CONTACT
export@funakoshi.co.jp
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