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4 unique monoclonal antibodies for cancer-related laminin chains Anti-laminin mouse monoclonal antibodies | Funakoshi

Date:February 01 2019Web Page No:81185

Funakoshi Co.,Ltd.

Useful monoclonal antibodies for the research of cancer pathology
4 unique monoclonal antibodies against cancer-related laminin chains

Laminins are major extracellular matrix proteins containing over 15 members. Laminins are large hetero-trimers consisting of 5 alpha chains, 3 beta chains, and 3 gamma chains. Due to its structural varieties, it has been challenging to detect each laminin isoform by conventional antibodies.

Funakoshi commercializes 4 unique antibodies, originally developed by Dr. Kaoru Miyazaki, Professor Emeritus of Yokohama City University in Japan.
Our antibodies can separately recognize specific isoforms or structures of laminin.

Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 and P2H).

Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 and P2H).


Features

BG5/F7 : For laminin alpha-3 chains

- Laminin alpha 3 chain, a major component of Laminin-332, has two splicing variants, alpha 3A and alpha 3B
- Clone BG5 is specific antibody for alpha 3A
- Clone F7 is the world first commercially available antibody for alpha 3B

P2H : For laminin gamma-2 chain

- Laminin gamma2, an only component of Laminin-332, is a marker gene of invasive cancer tissue
- Laminin gamma-2 chain is activated via proteolytical cleavage at N-terminal
- N-terminal proteolytic fragment of gamma-2, called γ2pf, is highly accumulated into cytosol under malignant cancer
- Clone P2H is the world first antibody for γ2pf

12D : For laminin-511

- There are two similar isoforms, laminin-511 and laminin-521 and it is difficult for conventional antibodies such as anti-alpha 5 chain to separate them.
- Clone 12D specifically react with trimeric structure of laminin-511, not react with each components alpha 5, beta 1, gamma 1 and similar isoform laminin-511.

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Note

Laminin 511 is used as a culture substrate for ES and iPS cells. (See our iMatrix-511 / Easy iMatrix-511).

Fig.2 Structure of Laminin-511

Fig.2 Structure of Laminin-511


clone BG5 F7 P2H 12D
Target Laminin-α3A chain Laminin-α3B chain Laminin-γ2 chain N-terminal region Laminin-511 trimer
Normal Tissue
Epitope
Cancer Tissue

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Outline of four clones

DescriptionAnti-Laminin alpha 3AAnti-Laminin alpha 3BAnti-Laminin gamma 2 N-terminal FragmentAnti-Laminin-511
CloneBG5F7P2H12D
SpecificityLaminin alpha 3A*1Laminin alpha 3BLaminin gamma 2 Domain V-NE2Laminin-511
Host and TypeMouse Monoclonal
SubclassIgG2aIgG1IgG2aIgG2b
ReactivityHumanHumanHuman and MouseHuman
FormMouse Ascites (Unpurifed)
ApplicationELISA, IHC, IP, WBIHC (frozen & paraffin sections), WBIHC (frozen), IP, WB (Non-reducing) *2
CodeFDV-0024FDV-0023FDV-0025FDV-0026
Size100 uL
Storage-20 ℃ *3

*1 Clone BG5 only detects alpha 3A in IHC. However, this clone may react with alpha 3B in ELISA, IP and Western Blot.
*2 Clone 12D can be only used for IHC (Frozen) and under non-reducing condition for Western Blot.
*3 Non-preservative added. So these 4 clones can be degraded at 4C. We recommend -20C storage.

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Data Examples

Fig.3  IHC image of skin cancer tissue in clone BG5 and F7

Fig.3 IHC image of skin cancer tissue in clone BG5 and F7
Left : Clone BG5, Right : Clone F7
Both clones detected basal membrane like structure around the tumor but F7 clone also detected vascular basement membrane (arrow heads).


Fig.4 IHC image of tissues in clone P2H

Fig.4 IHC image of tissues in clone P2H
Upper images : Normal mammary gland
Lower images : Breast cancer
P2H stains basement membranes surrouding mammary glands in normal tisue, whereas in a breast cancer tissue cytoplasmic accumulation of γ2pf orits fragments is detected.


Fig.5 IHC image of breast cancer tissue in clone 12D

Fig.5 IHC image of breast cancer tissue in clone 12D
A : Vascular basement membrane
B : Mammary gland basement membrane
C : Basement membrane-like structures surrounding tumor cells


Fig.6 Specificity of clone 12D against laminin isoforms

Fig.6 Specificity of clone 12D against laminin isoforms
Seven recombinant human laminins were coated on a multi-well plate and detected by clone 12D. The data shows clone 12D specifically reacts with Laminin 511.


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Price

[Date : June 20 2024 00:07]

Detail Product Name Product Code Supplier Size Price
Anti-Laminin alpha 3B, Human, Mouse-Mono (F7)
DatasheetThis may not be the latest data sheet.
FDV-0023 FNAFunakoshi Co.,Ltd. 100 µl $400

Description
Storage -80°C
Antigen Species Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human Application ELISA,IHC,IP,Western Blot
Clonality Format Purification Acsites Absorption
Link

Anti-Laminin alpha 3A, Human, Mouse-Mono (BG5)
DatasheetThis may not be the latest data sheet.
FDV-0024 FNAFunakoshi Co.,Ltd. 100 µl $400

Description
Storage -20°C
Antigen Species Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human Application ELISA,IHC,IP,Western Blot
Clonality Format Purification Acsites Absorption
Link

Anti-Laminin gamma 2 N-terminal fragment, Human, Mouse-Mono (P2H)
DatasheetThis may not be the latest data sheet.
FDV-0025 FNAFunakoshi Co.,Ltd. 100 µl $400

Description
Storage -20°C
Antigen Species Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human/Mouse Application IHC,Western Blot
Clonality Format Purification Acsites Absorption
Link

Anti-Laminin 511, Human, Mouse-Mono (12D)
DatasheetThis may not be the latest data sheet.
FDV-0026 FNAFunakoshi Co.,Ltd. 100 µl $400

Description
Storage -80°C
Antigen Species Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human Application IHC(frozen),IP,Western Blot
Clonality Format Purification Acsites Absorption
Link

[Date : June 20 2024 00:07]

Anti-Laminin alpha 3B, Human, Mouse-Mono (F7)


  • Product Code: FDV-0023
  • Supplier: FNA
  • Size: 100µl
  • Price: $400

Description
Storage -80°C
Antigen Species Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human Application ELISA,IHC,IP,Western Blot
Clonality Format
Purification Acsites Absorption
Link

Anti-Laminin alpha 3A, Human, Mouse-Mono (BG5)


  • Product Code: FDV-0024
  • Supplier: FNA
  • Size: 100µl
  • Price: $400

Description
Storage -20°C
Antigen Species Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human Application ELISA,IHC,IP,Western Blot
Clonality Format
Purification Acsites Absorption
Link

Anti-Laminin gamma 2 N-terminal fragment, Human, Mouse-Mono (P2H)


  • Product Code: FDV-0025
  • Supplier: FNA
  • Size: 100µl
  • Price: $400

Description
Storage -20°C
Antigen Species Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human/Mouse Application IHC,Western Blot
Clonality Format
Purification Acsites Absorption
Link

Anti-Laminin 511, Human, Mouse-Mono (12D)


  • Product Code: FDV-0026
  • Supplier: FNA
  • Size: 100µl
  • Price: $400

Description
Storage -80°C
Antigen Species Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human Application IHC(frozen),IP,Western Blot
Clonality Format
Purification Acsites Absorption
Link



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Citations and References

  1. Kariya, Y., et al., J. Mol. Histol., 39, 435-446 (2008) (Clone BG5/F7)
  2. Mori, T., et al., Cancer Sci., 102, 1095-1100 (2011) (Clone F7)
  3. Miyazaki, K., et al., Cancer Sci., 107, 1909-1918 (2016) (Clone BG5/P2H)
  4. Komiya, E., et al., Cancer Med., 3, 537-549 (2014) (Clone 12D)

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CONTACT

export@funakoshi.co.jp

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