抗ATR (phospho Thr1989)抗体 | Anti-ATR (phospho Thr1989) antibody

掲載日情報:2020/04/02 現在Webページ番号:45521

GeneTex社の抗ATR (phospho Thr1989)抗体 | Anti-ATR (phospho Thr1989) antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗ATR (phospho Thr1989)抗体(#GTX128145)のユーザーレビューはこちら。

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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月05日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-ATR (pThr1989), Phosphorylated, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 73
説明文
レビューあり
別名:ATR serine/threonine kinase,FCTCS,FRP1,MEC1,SCKL,SCKL1
Genbank No: 545
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 GeneTex社 DNA損傷/修復関連抗体
細胞周期・微小管研究用製品特集
関連記事 GeneTex社における抗体の品質管理
Anti-ATR (pThr1989), Phosphorylated, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 73
説明文
レビューあり
別名:ATR serine/threonine kinase,FCTCS,FRP1,MEC1,SCKL,SCKL1
Genbank No: 545
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 GeneTex社 DNA損傷/修復関連抗体
細胞周期・微小管研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月05日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-ATR (pThr1989), Phosphorylated, Rabbit-Poly

文献数: 73

説明文 レビューあり
別名:ATR serine/threonine kinase,FCTCS,FRP1,MEC1,SCKL,SCKL1
Genbank No: 545
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 GeneTex社 DNA損傷/修復関連抗体
細胞周期・微小管研究用製品特集
関連記事 GeneTex社における抗体の品質管理

Anti-ATR (pThr1989), Phosphorylated, Rabbit-Poly

文献数: 73

説明文 レビューあり
別名:ATR serine/threonine kinase,FCTCS,FRP1,MEC1,SCKL,SCKL1
Genbank No: 545
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 GeneTex社 DNA損傷/修復関連抗体
細胞周期・微小管研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody (GTX128145).Western blot analysis was performed using ATR (phospho Thr1989) antibody (GTX128145).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:2000.

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IP
ReactivityHuman, Mouse


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW 301 kDa. ( Note )
Positive Control HCT116 , HCT116 treated 1mM Hydroxyurea , Raji , Raji nuclear extract


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Carrier-protein conjugated synthetic peptide corresponding to residues around human ATR (phospho Thr1989). The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1
Cellular Localization Nucleus
Background The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]
Database ・ Gene ID: 545 ATR
・ UniProt: Q13535 ATR


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Rikitake M et al. Genes Cells 2020; (Epub) MLH1-mediated recruitment of FAN1 to chromatin for the induction of apoptosis triggered by O6 -methylguanine.Application : WB
Reactivity : Human
Kim W et al. Nat Commun 2019; 10 (1) : 5304 ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex.Application : WB
Reactivity : Human
Tang M et al. Sci Adv 2019; 5 (3) : eaav1118 SIRT7-mediated ATM deacetylation is essential for its deactivation and DNA damage repair.
Fragkos M et al. Oncotarget 2019; 10 (43) : 4407-4423 Dicer prevents genome instability in response to replication stress.Application : WB
Reactivity : Human
Bigot N et al. Elife 2019; 8 Phosphorylation-mediated interactions with TOPBP1 couple 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint.


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