抗Grp78抗体 | Anti-Grp78 antibody
掲載日情報:2020/04/13 現在Webページ番号:40811
GeneTex社の抗Grp78抗体 | Anti-Grp78 antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月25日 18時55分現在]
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Anti-Grp78, Rabbit |
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Anti-Grp78, Rabbit |
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[在庫・価格 :2025年04月25日 18時55分現在]
Anti-Grp78, Rabbit
文献数: 46
- 商品コード:GTX113340
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。 別名:heat shock protein family A (Hsp70) member 5,BIP,GRP78,HEL-S-89n,MIF2 Genbank No: 3309 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Chicken/Human/Mouse/Rat | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 小胞体ストレス関連抗体 小胞体(Endoplasmic Reticulum:ER)ストレス研究用製品特集 小胞体、ゴルジ体、リポソーム、ドラッグデリバリーシステムの研究に!細胞内輸送特集 脂質過酸化研究用製品特集 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-Grp78, Rabbit
文献数: 46
- 商品コード:GTX113340
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。 別名:heat shock protein family A (Hsp70) member 5,BIP,GRP78,HEL-S-89n,MIF2 Genbank No: 3309 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Chicken/Human/Mouse/Rat | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 小胞体ストレス関連抗体 小胞体(Endoplasmic Reticulum:ER)ストレス研究用製品特集 小胞体、ゴルジ体、リポソーム、ドラッグデリバリーシステムの研究に!細胞内輸送特集 脂質過酸化研究用製品特集 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | ![]() Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 antibody (GTX113340) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody (GTX113340) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm. |
![]() | Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113340 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500. Blue: Hoechst 33342 staining. |
![]() | Grp78 antibody detects Grp78 protein by western blot analysis. A. 30 μg PC-12 whole cell extract B. 30 μg Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody (GTX113340) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() The WB analysis of Grp78 antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494 |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P |
Reactivity | Human, Mouse, Rat, Chicken |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:5000-1:20000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
Calculated MW | 72 kDa. ( Note ) |
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Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 |
Predict Reactivity | Zebrafish, Bovine, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.61 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2 |
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Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Background | When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM] |
Database | ・ Gene ID: 3309 HSPA5 ・ UniProt: P11021 HSPA5 |
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REFERENCE
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