抗Glypican 1 [N3C3]抗体 | Anti-Glypican 1 [N3C3] antibody
掲載日情報：2020/04/02 現在Webページ番号：36869
GeneTex社の抗Glypican 1 [N3C3]抗体 | Anti-Glypican 1 [N3C3] antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗Glypican 1 [N3C3]抗体(#GTX104557)のユーザーレビューはこちら。
| Rating |  | ( Average 5 based on 1 users reviews) |
| Application | ||
| Western Blot(WB) | | ( Average 5 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 ：2024年04月30日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-GPC1, Rabbit-Poly |
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Anti-GPC1, Rabbit-Poly |
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[在庫・価格 ：2024年04月30日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-GPC1, Rabbit-Poly
文献数： 10
- 商品コード：GTX104557
- メーカー：GNT
- 包装：100μl
- 価格：¥75,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名：glypican 1，glypican Genbank No： 2817 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Human/Mouse/Rat | 適用 | ELISA,FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Glypican-1 Antibody GeneTex社 抗腫瘍マーカー抗体 -膵臓がん- GeneTex社 がん研究用抗体特集 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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Anti-GPC1, Rabbit-Poly
文献数： 10
- 商品コード：GTX104557
- メーカー：GNT
- 包装：25μl
- 価格：¥24,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名：glypican 1，glypican Genbank No： 2817 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Human/Mouse/Rat | 適用 | ELISA,FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
Glypican-1 Antibody GeneTex社 抗腫瘍マーカー抗体 -膵臓がん- GeneTex社 がん研究用抗体特集 使いっきり抗体 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
 |  Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product. |
 |  Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778 |
 | Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778 |
 | U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778 |
 | Mouse tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. |
 | An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL. |
 | Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (GTX104557).Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
 | U87-MG whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778 |
 | Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis. |
 | Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm. |
 |  Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 22351761 , PMID: 26203194 , PMID: 21932778 |
 | Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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製品情報
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Application | WB, ICC/IF, IHC-P, FACS, IP, ELISA |
| Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.| Application | Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:100-1:1000 |
| FACS | 1:50-1:200 |
| IP | 1:100-1:500 |
| ELISA | 1:1000-1:10000 |
| Calculated MW | 62 kDa. ( Note ) |
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| Positive Control | A431 , K562 , MCF-7 , AsPC-1 , MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 (500 units PNGase F for 4 hr) , A431 (24 μg/ml Tunicamycin treatment for 16hr) , A431 (4 mM Benzyl glucosinolate treatment for 32hr) , A431(24 μg/ml Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr) |
| Predict Reactivity | Bovine, Rhesus Monkey(>80% identity) |
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PROPERTIES
| Form | Liquid |
|---|---|
| Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Concentration | 0.33 mg/ml (Please refer to the vial label for the specific concentration.) |
| Antigen Species | Human |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
| Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
| Synonyms | glypican 1 , glypican |
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| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq] |
| Database | ・ Gene ID: 2817 GPC1 ・ UniProt: P35052 GPC1 |
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REFERENCEE(抜粋)
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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