抗PARP抗体 | Anti-PARP antibody
掲載日情報:2020/04/13 現在Webページ番号:35176
GeneTex社の抗PARP抗体 | Anti-PARP antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
GeneTex社 ホームページでユーザーレビューが閲覧できます
GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗PARP抗体(#GTX100573)のユーザーレビューはこちら。
Rating | ( Average 5 based on 1 users reviews) | |
Application | ||
Western Blot(WB) | ( Average 5 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2024年04月27日 00時00分現在]
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Anti-PARP1, Rabbit-Poly |
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[在庫・価格 :2024年04月27日 00時00分現在]
Anti-PARP1, Rabbit-Poly
文献数: 71
- 商品コード:GTX100573
- メーカー:GNT
- 包装:25μl
- 価格:¥24,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 151~385 別名:poly(ADP-ribose) polymerase 1,ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1 Genbank No: 142 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
GeneTex社 DNA損傷/修復関連抗体 細胞周期・微小管研究用製品特集 使いっきり抗体 |
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関連記事 |
GeneTex社における抗体の品質管理 |
Anti-PARP1, Rabbit-Poly
文献数: 71
- 商品コード:GTX100573
- メーカー:GNT
- 包装:100μl
- 価格:¥75,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 151~385 別名:poly(ADP-ribose) polymerase 1,ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1 Genbank No: 142 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
GeneTex社 DNA損傷/修復関連抗体 細胞周期・微小管研究用製品特集 |
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関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
PARP1 antibody detects PARP1 protein by western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr) 7.5% SDS-PAGE PARP1 antibody (GTX100573) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody (GTX100573) diluted at 1:2000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. | |
PARP antibody detects PARP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP protein stained by PARP antibody (GTX100573) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm. | |
PARP antibody detects PARP protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. PARP antibody (GTX100573) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min | |
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter. ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48. | |
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. | |
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
The WB analysis of PARP antibody was published by Li CJ and colleagues in the journal Evid Based Complement Alternat Med in 2012.PMID: 23091557 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay |
Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | Assay dependent |
IP | Assay dependent |
ChIP assay | Assay dependent |
Calculated MW | 113 kDa. ( Note ) |
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Positive Control | 293T , A431 , HeLa , HepG2 , HeLa (30 μM cisplatin treatment for 24 hr) , HCT116 , HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 , |
Predict Reactivity | Bovine, Chicken(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.3 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1 |
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Cellular Localization | Nucleus,Nucleolus |
Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
Database | ・ Gene ID: 142 PARP1 ・ UniProt: P09874 PARP1 |
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REFERENCE
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