抗IL-33抗体(Anti-IL-33, Goat-Poly antibody)
掲載日情報:2018/11/26 現在Webページ番号:33286
IL-33に対する抗体(Anti-IL-33, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2025年06月23日 00時00分現在]
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Anti-IL-33, Goat-Poly |
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Anti-Mouse IL-33 Affinity Purified Polyclonal Ab |
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[在庫・価格 :2025年06月23日 00時00分現在]
Anti-IL-33, Goat-Poly
文献数: 58
- 商品コード:AF3626
- メーカー:RSD
- 包装:100μg
- 価格:¥106,000
- 在庫:2個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse IL-33 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF3626,スタンダードとして#3626-ML-010を用いる。 別名:C9orf26 Genbank No: 90865 Protein Accession No: Q8BVZ5 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Mouse | 免疫動物 | Goat | ||||
交差性 | Mouse | 適用 | ELISA,FCM,IC,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IL-33 Affinity Purified Polyclonal Ab
文献数: 10
- 商品コード:AF3626-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:C9orf26 Genbank No: 90865 Protein Accession No: Q8BVZ5 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Goat | |||||
交差性 | Mouse | 適用 | ELISA,FCM,IC,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant mouse IL-33Ser109-Ile266Accession # Q8BVZ5 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL‑33 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.4 µg/mL | See below |
Immunohistochemistry | 5-15 µg/mL | See below |
CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
Immunocytochemistry | 5-15 µg/mL | See below |
Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | See below |
Mouse IL-33 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse IL‑33 Antibody (Catalog #AF3626 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑33 Biotinylated Antibody (Catalog #BAF3626 ) |
ELISA Standard | Recombinant Mouse IL-33 Protein (Catalog #3626-ML ) | |
Neutralization | Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑33. |
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Data Examples
Western Blot | ![]() click image to view larger | Detection of Mouse IL‑33 by Western Blot.Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
Immunohistochemistry | ![]() click image to view larger | IL‑33 in Mouse Spleen.IL‑33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
Intracellular Staining by Flow Cytometry | ![]() click image to view larger | Detection of IL‑33 in Mouse Splenocytes by Flow Cytometry.Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 μg/mL Ca2+ ionomycin then stained with Goat Anti-Mouse IL‑33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
Immunocytochemistry | ![]() click image to view larger | IL‑33 in bEnd.3 Mouse Cell Line.IL‑33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
Neutralization | ![]() click image to view larger | Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody.Recombinant Mouse IL‑33 (Catalog # 3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND50 is typically 10-50 ng/mL. |
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