抗Bovine IFN-gamma Biotinylated抗体(Anti-Bovine IFN-gamma Biotinylated antibody)
掲載日情報：2021/01/28 現在Webページ番号：32474
Bovine IFN-gamma Biotinylatedに対する抗体(Anti-Bovine IFN-gamma Biotinylated )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Bovine IFN-gamma Biotinylated Affinity Purified PAb (50 UG) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Bovine IFN-gamma Biotinylated Affinity Purified PAb (50 UG)
文献数： 0
- 商品コード：BAF2300
- メーカー：RSD
- 包装：50μg
- 価格：¥111,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Bovine IFN-γ サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB23001，スタンダードとして#2300-BG-025を用いる。 別名：IFG Genbank No： 3458 Protein Accession No： NP_776511 |
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| 保存条件 | 法規備考 | ||
| 抗原種 | Bovine | 免疫動物 | Goat |
| 交差性 | Bovine | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Bovine |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant bovine IFN-gamma Gln24-Thr166Accession # NP_776511 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects bovine IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IFN-gamma, recombinant mouse IFN-gamma, and recombinant porcine IFN-gamma is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Bovine IFN-gamma (Catalog # 2300-BG) |
| Bovine IFN-gamma Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Bovine IFN‑ gamma Antibody (Catalog #MAB23001 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Bovine IFN‑ gamma Biotinylated Antibody (Catalog #BAF2300 ) |
| ELISA Standard | Recombinant Bovine IFN-gamma Protein (Catalog #2300-BG ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IFN-gamma | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). | |
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References
| Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. | |
| Pestka, S. et al. (2004) Immunol. Rev. 202:8. | |
| Cerretti, D.P. et al. (1986) J. Immunol. 136:4561. | |
| Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. | |
| Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. | |
| Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. | |
| McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. | |
| Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. | |
| Kelchtermans, H. et al. (2008) Trends Immunol. 29:479. | |
| Long Name: | Interferon gamma |
| Entrez Gene IDs: | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Alternate Names: | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
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Related Research Areas
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Related Product & Information
| Background | IFN-gamma |
|---|---|
| background_content | Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
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