抗Syndecan-1抗体(Anti-Syndecan-1, Goat-Poly, Biotin antibody)

掲載日情報:2021/01/28 現在Webページ番号:31984

Syndecan-1に対する抗体(Anti-Syndecan-1, Goat-Poly, Biotin )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年05月18日 09時15分現在]

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納期 文献数
Anti-Syndecan-1, Goat-Poly, Biotin
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
マッチドペア:Human Syndecan-1/CD138 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF2780,スタンダードとして#2780-SD-050を用いる。
別名:CD138
Genbank No: 6382
Protein Accession No: NP_002988
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Biotin
交差性 Human 適用 ELISA,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2024年05月18日 09時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Syndecan-1, Goat-Poly, Biotin

文献数: 1

説明文 マッチドペア:Human Syndecan-1/CD138 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF2780,スタンダードとして#2780-SD-050を用いる。
別名:CD138
Genbank No: 6382
Protein Accession No: NP_002988
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,IHC,Western Blot
標識 Biotin 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityHuman
LabelBiotin
ImmunogenMouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138Gln18-Glu251Accession # NP_002988
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4,and recombinant mouse Syndecan-1 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)
Immunohistochemistry5-15 µg/mLSee below
Human Syndecan-1/CD138 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)0.2-0.8 µg/mL Human Syndecan‑1/CD138 Antibody (Catalog #AF2780 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog #BAF2780 )
ELISA Standard  Recombinant Human Syndecan-1 Protein, CF (Catalog #2780-SD )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry Immunohistochemistry Syndecan-1/CD138 Antibody [Biotin]
click image to view larger
Syndecan‑1/CD138 in Human Intestine.Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human intestine using Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

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References

Entrez Gene IDs:6382 (Human); 20969 (Mouse); 100338470 (Rabbit)
Alternate Names:CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1

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Related Research Areas

B Cell Markers
Cancer Biomarkers
Cancer Stem Cell Markers
Mesenchymal Cells and EMT
Synaptic Proteins
  1. Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
    Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
    Mol. Biol. Cell, 2007;18(8):3119-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB


Immunohistochemistry
Syndecan‑1/CD138 in Human Intestine.
Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human intestine using Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.


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Related Product & Information

BackgroundSyndecan-1/CD138
background_contentBackground:
Syndecan-1/CD138
Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
    Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
    Mol. Biol. Cell, 2007;18(8):3119-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB



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