抗Syndecan-1抗体(Anti-Syndecan-1, Goat-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:31984
Syndecan-1に対する抗体(Anti-Syndecan-1, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2024年05月18日 09時15分現在]
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Anti-Syndecan-1, Goat-Poly, Biotin |
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[在庫・価格 :2024年05月18日 09時15分現在]
Anti-Syndecan-1, Goat-Poly, Biotin
文献数: 1
- 商品コード:BAF2780
- メーカー:RSD
- 包装:50μg
- 価格:¥118,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Human Syndecan-1/CD138 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF2780,スタンダードとして#2780-SD-050を用いる。 別名:CD138 Genbank No: 6382 Protein Accession No: NP_002988 |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | Human | 免疫動物 | Goat |
交差性 | Human | 適用 | ELISA,IHC,Western Blot |
標識 | Biotin | 性状 | Antigen Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Polyclonal | フォーマット | |
掲載カタログ |
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製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Biotin |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138Gln18-Glu251Accession # NP_002988 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4,and recombinant mouse Syndecan-1 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD) |
Immunohistochemistry | 5-15 µg/mL | See below |
Human Syndecan-1/CD138 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human Syndecan‑1/CD138 Antibody (Catalog #AF2780 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog #BAF2780 ) |
ELISA Standard | Recombinant Human Syndecan-1 Protein, CF (Catalog #2780-SD ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Immunohistochemistry |
click image to view larger | Syndecan‑1/CD138 in Human Intestine.Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human intestine using Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Syndecan-1/CD138 | Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1. |
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References
Entrez Gene IDs: | 6382 (Human); 20969 (Mouse); 100338470 (Rabbit) |
Alternate Names: | CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1 |
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Related Research Areas
B Cell Markers | ||
Cancer Biomarkers | ||
Cancer Stem Cell Markers | ||
Mesenchymal Cells and EMT | ||
Synaptic Proteins | ||
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Related Product & Information
Background | Syndecan-1/CD138 |
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background_content | Background: Syndecan-1/CD138 Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1. |
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Citations
- Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
Mol. Biol. Cell, 2007;18(8):3119-30.
Species: Human
Sample Type: Cell Lysates
Application: WB
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