抗CD36/SR-B3抗体(Anti-CD36/SR-B3, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3Gly30-Lys439Accession # Q3UAI3
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CD36/SR-B3 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant human CD36 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD)
Flow Cytometry	2.5 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Mouse CD36/SR-B3 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CD36/SR‑B3 Antibody (Catalog #AF2519 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CD36/SR‑B3 Biotinylated Antibody (Catalog #BAF2519 )
ELISA Standard		Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF (Catalog #2519-CD )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Flow Cytometry	click image to view larger	Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry.J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD36/SR-B3	CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).
Long Name:	Scavenger Receptor Class B, Member 3
Entrez Gene IDs:	948 (Human); 12491 (Mouse)
Alternate Names:	CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

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Related Research Areas

Alpha Granule Membrane Proteins

Endothelial Cell Markers

Immune Regulatory Molecules on Endothelial Cells

Leukocyte Receptors/Markers in Allergy and Asthma

Macrophage Activation Markers

Monocyte Markers

Mouse Tissue-Resident Macrophage Markers

Myeloid Lineage Markers

Platelet Adhesion Molecules

Red Blood Cells (RBCs)

Scavenger Receptors

Toll-Like Receptors

Uptake and Transport Molecules in VSMC

Uptake and Transport Molecules on Endothelial Cells

Uptake of Amyloid beta by Glia Cells

Uptake of Amyloid beta by Macrophages

A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity Authors: S Tsuzuki, Y Kimoto, S Lee, T Sugawara, Y Manabe, K Inoue Biomed. Res., 2018;39(3):117-129. Species: Mouse Sample Type: Tissue Homogenates Application: WB

Flow Cytometry

Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CD36/SR‑B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2519, filled histogram) or isotype control antibody (Catalog #AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

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Related Product & Information

Background

CD36/SR-B3

background_content

Background: CD36/SR-B3 CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84%, and 84% aa identical to the extracellular loops of rat, hamster, human, and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells, and hematopoietic cells such as red blood cells, platelets, and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

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Citations

1. A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity
   Authors: S Tsuzuki, Y Kimoto, S Lee, T Sugawara, Y Manabe, K Inoue
   Biomed. Res., 2018;39(3):117-129.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. 2Intestinal epithelial cell Caveolin 1 regulates fatty acid and lipoprotein cholesterol plasma levels
   Authors: JP Otis, MC Shen, V Quinlivan, JL Anderson, SA Farber
   Dis Model Mech, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - PFA fixed


3. CD36 is essential for endurance improvement, changes in�whole-body metabolism, and efficient PPAR-related transcriptional responses in the muscle with exercise training
   Authors: MCC Manio, S Matsumura, D Masuda, K Inoue
   Physiol Rep, 2017;5(10):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. CCN3 Regulates Macrophage Foam Cell Formation and Atherosclerosis
   Authors: H Shi, C Zhang, V Pasupuleti, X Hu, DA Prosdocimo, W Wu, Y Qing, S Wu, H Mohammad, SL Gerson, B Perbal, PA Klenotic, N Dong, Z Lin
   Am. J. Pathol., 2017;187(6):1230-1237.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Cell-surface CD36 in monocyte/macrophage contributes to phagocytosis during the resolution phase of ischemic stroke in mice
   J Biol Chem, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
   Authors: R Biedro?, A Peru?, S Józefowski
   PLoS ONE, 2016;11(4):e0153558.
   Species: Mouse
   Sample Type: Recombinant Protein
   Application: Binding Assay


7. Expression of CD36 by Olfactory Receptor Cells and Its Abundance on the Epithelial Surface in Mice.
   Authors: Lee S, Eguchi A, Tsuzuki S, Matsumura S, Inoue K, Iwanaga T, Masuda D, Yamashita S, Fushiki T
   PLoS ONE, 2015;10(7):e0133412.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Regulation of AMPK activation by CD36 links fatty acid uptake to beta-oxidation.
   Authors: Samovski D, Sun J, Pietka T, Gross R, Eckel R, Su X, Stahl P, Abumrad N
   Diabetes, 2015;64(2):353-9.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
   Authors: Pietka, Terri A, Sulkin, Matthew, Kuda, Ondrej, Wang, Wei, Zhou, Dequan, Yamada, Kathryn, Yang, Kui, Su, Xiong, Gross, Richard, Nerbonne, Jeanne M, Efimov, Igor R, Abumrad, Nada A
   J Biol Chem, 2012;287(46):38901-12.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice.
    Authors: Nagashima M, Watanabe T, Terasaki M
    Diabetologia, 2011;54(10):2649-59.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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