抗IFN-γ抗体(Anti-IFN-γ, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：31204
IFN-γに対する抗体(Anti-IFN-γ, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年12月13日 13時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IFN-γ, Goat-Poly |
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Anti-Equine IFN-gamma Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年12月13日 13時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IFN-γ, Goat-Poly
文献数： 0
- 商品コード：AF1586
- メーカー：RSD
- 包装：100μg
- 価格：¥78,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Equine IFN-γ サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1586，スタンダードとして#1586-HG-025を用いる。 別名：IFG Genbank No： 3458 Protein Accession No： P42160 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | Equine | 免疫動物 | Goat | ||||
| 交差性 | Equine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Equine IFN-gamma Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1586-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥25,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：IFG Genbank No： 3458 Protein Accession No： P42160 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Equine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Equine |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant equine IFN-gamma Ala25-Gln166Accession # P42160 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects equine IFN‑ gamma in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross‑reactivity with recombinant canine IFN‑ gamma is observed, less than 6% cross‑reactivity with recombinant bovine IFN‑ gamma and recombinant feline IFN‑ gamma is observed, and less than 0.2% cross‑reactivity with recombinant human IFN‑ gamma, recombinant mouse IFN‑ gamma, recombinant rat IFN‑ gamma, recombinant porcine IFN‑ gamma, recombinant rhesus macaque IFN‑ gamma, and recombinant cotton rat IFN‑ gamma is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Equine IFN-gamma (Catalog # 1586-HG) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Equine IFN-gamma Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Equine IFN‑ gamma Antibody (Catalog #AF1586 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Equine IFN‑ gamma Biotinylated Antibody (Catalog #BAF1586 ) |
| ELISA Standard | Recombinant Equine IFN-gamma Protein (Catalog #1586-HG ) | |
| Neutralization | Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 20 ng/mL Recombinant Equine IFN‑ gamma. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization IFN-gamma Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IFN-gamma_AF1586_Block_Neutralize_8785.jpg) click image to view larger | IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Equine IFN‑ gamma Antibody. Recombinant Equine IFN‑ gamma (Catalog # 1586‑HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose‑dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND50 is typically 0.25-1.25 µg/mL. |
| Immunocytochemistry |
![Immunocytochemistry IFN-gamma Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IFNgamma_AF1586_Immunocytochemistry__Immunofluorescence_18845.jpg) click image to view larger | IFN‑ gamma in Equine PBMCs.IFN‑ gamma was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IFN-gamma | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature equine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3, 4). It shares 73%‑82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-gamma and 42%‑64% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8). | |
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References
| Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. | |
| Pestka, S. et al. (2004) Immunol. Rev. 202:8. | |
| Grunig, G. et al. (1994) Immunogenetics 39:448. | |
| Curran, J.A. et al. (1994) DNA Seq. 4:405. | |
| Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. | |
| Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. | |
| Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. | |
| McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. | |
| Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. | |
| Kelchtermans, H. et al. (2008) Trends Immunol. 29:479. | |
| Long Name: | Interferon gamma |
| Entrez Gene IDs: | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Alternate Names: | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
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Related Research Areas
| Neutralization | |
|---|---|
 | IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Equine IFN‑ gamma Antibody. Recombinant Equine IFN‑ gamma (Catalog # 1586‑HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose‑dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND50 is typically 0.25-1.25 µg/mL. |
| Immunocytochemistry | |
 | IFN‑ gamma in Equine PBMCs. IFN‑ gamma was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Long Name | Interferon gamma |
|---|---|
| Entrez Gene IDs | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Background | IFN-gamma |
| background_content | Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature equine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3, 4). It shares 73%‑82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-gamma and 42%‑64% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8). |
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