抗IL-2 Receptor α抗体(Anti-IL-2 Receptor α, Goat-Poly antibody)
掲載日情報:2021/01/28 現在Webページ番号:30863
IL-2 Receptor αに対する抗体(Anti-IL-2 Receptor α, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 :2024年05月18日 00時00分現在]
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Anti-IL-2 Receptor α, Goat-Poly |
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Anti-Mouse CD25/IL-2 R alpha Affinity Purified Polyclonal Ab |
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[在庫・価格 :2024年05月18日 00時00分現在]
Anti-IL-2 Receptor α, Goat-Poly
文献数: 2
- 商品コード:AF2438
- メーカー:RSD
- 包装:100μg
- 価格:¥106,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Mouse CD25/IL-2 Rα サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF2438,スタンダードとして#2438-RM-050を用いる。 別名:CD25 Genbank No: 3559 Protein Accession No: Q544I2 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Mouse | 免疫動物 | Goat | ||||
交差性 | Mouse | 適用 | ELISA,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse CD25/IL-2 R alpha Affinity Purified Polyclonal Ab
文献数: 2
- 商品コード:AF2438-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
※受注発注品。形状:溶液または凍結乾燥 別名:CD25 Genbank No: 3559 Protein Accession No: Q544I2 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Goat | |||||
交差性 | Mouse | 適用 | ELISA,IHC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha Glu22-Lys236Accession # Q544I2 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human CD25/IL-2 R alpha is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Mouse CD25/IL‑2 R alpha (Catalog # 2438-RM) |
Immunohistochemistry | 5-15 µg/mL | See below |
Mouse CD25/IL-2 R alpha Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse CD25/IL-2 R alpha Antibody (Catalog #AF2438 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CD25/IL-2 R alpha Biotinylated Antibody (Catalog #BAF2438 ) |
ELISA Standard | Recombinant Mouse CD25/IL-2 R alpha Protein, CF (Catalog #2438-RM ) | |
Neutralization | Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 2 ng/mL Recombinant Mouse IL‑2. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
click image to view larger | Cell Proliferation Induced by IL‑2 and Neutralization by Mouse CD25IL‑2 R alpha Antibody. Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND50 is typically 5-20 µg/mL. |
Immunohistochemistry |
click image to view larger | CD25/IL‑2 R alpha in Mouse Spleen. CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of mouse spleen using 5 µg/mL Goat Anti-Mouse CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: CD25/IL-2 R alpha | IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-14). |
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References
Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245. | |
Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67. | |
Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638. | |
Miller, J. et al. (1985) J. Immunol. 134:4212. | |
Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505. | |
Hatakeyama, M. et al. (1989) Science 244:551. | |
Takeshita, T. et al. (1992) Science 257:379. | |
Wang, X. et al. (2005) Science 310:1159. | |
Wagner, D.K. et al. (1986) J. Immunol. 137:592. | |
Schulz, O. et al. (1998) J. Exp. Med. 187:271. | |
Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121. | |
Willerford, D.M. et al. (1995) Immunity 3:521. | |
Van Parijs, L. et al. (1997) J. Immunol. 158:3738. | |
Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850. | |
Long Name: | Interleukin 2 Receptor alpha |
Entrez Gene IDs: | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) |
Alternate Names: | CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR |
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Related Research Areas
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Related Product & Information
Long Name | Interleukin 2 Receptor alpha |
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Entrez Gene IDs | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) |
Background | CD25/IL-2 R alpha |
background_content | Background: CD25/IL-2 R alpha IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-14). |
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