抗IL-6抗体(Anti-IL-6, Canine, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：30340
IL-6に対する抗体(Anti-IL-6, Canine, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-IL-6, Canine, Goat-Poly, Biotin
文献数： 1
- 商品コード：BAF1609
- メーカー：RSD
- 包装：50μg
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Canine IL-6 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF1609，スタンダードとして#1609-CL-025を用いる。 別名：B cell stimulatory factor-2 Genbank No： 3569 Protein Accession No： P41323 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Canine | 免疫動物 | Goat |
| 交差性 | Canine | 適用 | ELISA,IC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Canine |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant canine IL‑6Thr23-Met207Accession # P41323 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects canine IL‑6 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant feline IL-6 and recombinant porcine IL‑6 is observed and less than 0.2% cross-reactivity with recombinant human IL-6, recombinant mouse IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, and recombinant equine IL-6 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Canine IL‑6 (Catalog # 1609-CL) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Canine IL-6 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Canine IL‑6 Antibody (Catalog #AF1609 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Canine IL‑6 Biotinylated Antibody (Catalog #BAF1609 ) |
| ELISA Standard | Recombinant Canine IL-6 Protein (Catalog #1609-CL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry IL-6 Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/IL6_BAF1609_Immunocytochemistry__Immunofluorescence_19413.jpg) click image to view larger | IL‑6 in Canine PBMCs.IL‑6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1609) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-6 | Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature canine IL-6 is 187 amino acids (aa) in length and shares 76%, 59%, 38%, and 40% aa sequence identity with feline, human, mouse, and rat IL-6, respectively (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8). |
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References
| Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. | |
| Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. | |
| Jones, S.A. (2005) J. Immunol. 175:3468. | |
| Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. | |
| Kukielka, G.L. et al. (1995) Circulation 92:1866. | |
| Murakami, M. et al. (1993) Science 260:1808. | |
| Muller-Newen, G. (2003) Sci. STKE 2003:PE40. | |
| Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. | |
| Long Name: | Interleukin 6 |
| Entrez Gene IDs: | 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit) |
| Alternate Names: | B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A |
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Related Research Areas
| Immunocytochemistry | |
|---|---|
 | IL‑6 in Canine PBMCs. IL‑6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1609) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | IL-6 |
|---|---|
| background_content | Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature canine IL-6 is 187 amino acids (aa) in length and shares 76%, 59%, 38%, and 40% aa sequence identity with feline, human, mouse, and rat IL-6, respectively (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8). |
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Citations
- Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
Authors: Wood MW, Breitschwerdt EB, Gookin JL
Infect. Immun., 2011;79(2):708-15.
Species: Canine
Sample Type: Whole Tissue
Application: IHC Frozen
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