抗Mouse CD3抗体(Anti-Mouse CD3 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	T cell hybridoma D1
Source	Monoclonal Rat IgG2B Clone # 17A2
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	Mouse splenocytes
Immunohistochemistry	8-25 µg/mL	See below
Immunoprecipitation	Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Complement-dependent Cytotoxicity	Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
T Cell Stimulation	Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Immunocytochemistry
	CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CD3 in Mouse Brain. CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	916 (Human); 12503 (Mouse)
Background	CD3
background_content	Background: CD3 The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

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Citations

1. CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
   Authors: MR Deyhle, PS Hafen, J Parmley, CN Preece, M Robison, JR Sorensen, B Jackson, DL Eggett, CR Hancock, RD Hyldahl
   Physiol Rep, 2018;6(8):e13689.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Nitrosative damage during retrovirus infection-induced neuropathic pain
   Authors: P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard
   J Neuroinflammation, 2018;15(1):66.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Crucial role of OX40/OX40L signaling in a murine model of asthma
   Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
   Mol Med Rep, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


4. A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice
   Authors: S Swirski, C Röger, A Pienkowska, C Ihlenburg, G Fischer, O May, M Vorm, M Owczarek-L, J Neidhardt
   Invest. Ophthalmol. Vis. Sci., 2018;59(1):561-571.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells
   Authors: W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng
   Parasit Vectors, 2017;10(1):348.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


6. Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy
   Authors: PG Makker, SS Duffy, JG Lees, CJ Perera, RS Tonkin, O Butovsky, SB Park, D Goldstein, G Moalem-Tay
   PLoS ONE, 2017;12(1):e0170814.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression
   Authors: X Fu, F Sun, F Wang, J Zhang, B Zheng, J Zhong, T Yue, X Zheng, JF Xu, CY Wang
   Mediators Inflamm., 2017;2017(0):5706152.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


8. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
   Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
   Sci Rep, 2017;7(1):6607.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


9. Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire
   Authors: David A Hildeman
   J. Immunol., 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


10. Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
    Authors: Ouyang M, White E, Ren H, Guo Q, Zhang I, Gao H, Yanyan S, Chen X, Weng Y, da Fonseca A, Shah S, Manuel E, Zhang L, Vonderfecht S, Alizadeh D, Berlin J, Badie B
    PLoS ONE, 2016;11(2):e0148139.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded

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