抗Canine IFN-gamma抗体(Anti-Canine IFN-gamma antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Canine
Label	Unconjugated
Immunogen	E. coli-derived recombinant canine IFN-gamma Gln24-Lys166Accession # P42161
Source	Monoclonal Mouse IgG2B Clone # 142529
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 75% cross‑reactivity with recombinant porcine IFN-gamma and no cross-reactivity with IFN-gamma from human, mouse, feline, rat, or rhesus macaque.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Canine IFN-gamma (Catalog # 781-CG)
Canine IFN-gamma Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Canine IFN‑ gamma Antibody (Catalog #MAB781 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Canine IFN‑ gamma Biotinylated Antibody (Catalog #BAM7811 )
ELISA Standard		Recombinant Canine IFN-gamma Protein (Catalog #781-CG )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79% - 88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62% - 73% with human, porcine, and rhesus IFN-gamma, and 40% - 47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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References

Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Zucker, K. et al. (1992) J. Interferon Res. 12:191.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name:	Interferon gamma
Entrez Gene IDs:	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:	IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Related Research Areas

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Group 1 ILCs

IL-10/Interferon Family

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Macrophage Activation Markers

Molecules Secreted by VSMC

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

NCR+ILC3

NCR-ILC3

Neuroinflammation

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant Vaccine, 2016;0(0):. Species: Canine Sample Type: Whole Cells Application: Flow

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Related Product & Information

Background

IFN-gamma

background_content

Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79% - 88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62% - 73% with human, porcine, and rhesus IFN-gamma, and 40% - 47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant
   Vaccine, 2016;0(0):.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


2. Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
   PLoS Negl Trop Dis, 2016;10(5):e0004614.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
   Authors: Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C
   J Immunol, 2013;191(11):5542-50.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


4. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
   Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
   Vaccine, 2007;25(21):4223-34.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
   Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G
   Vaccine, 2005;23(22):2825-40.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs.
   Authors: Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL
   Int. J. Parasitol., 2005;35(1):63-73.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
   Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
   Vaccine, 2005;24(4):501-9.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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