抗IFN-γ抗体(Anti-IFN-γ, Rat, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：29355
IFN-γに対する抗体(Anti-IFN-γ, Rat, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IFN-γ, Rat, Mouse-Mono(88928) |
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Anti-Rat IFN-gamma MAb (Clone 88928) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IFN-γ, Rat, Mouse-Mono(88928)
文献数： 3
- 商品コード：MAB5851
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat IFN-γ サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF585，スタンダードとして#585-IF-100を用いる。 別名：IFG クローン：88928 Genbank No： 3458 Protein Accession No： P01581 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Rat | 免疫動物 | Mouse | ||||
| 交差性 | Rat | 適用 | ELISA,IC | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Rat IFN-gamma MAb (Clone 88928)
文献数： 0
- 商品コード：MAB5851-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：IFG クローン：88928 Genbank No： 3458 Protein Accession No： P01581 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,IC | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rat IFN-gamma Gln23-Cys156Accession # P01581 |
| Source | Monoclonal Mouse IgG1 Clone # 88928 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects rat IFN-gamma in ELISAs. In sandwich ELISAs, observed cross-reactivities were less than 2% with recombinant mouse IFN‑ gamma and less than 0.1% with recombinant human IFN‑ gamma and recombinant porcine IFN‑ gamma. |
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Applications and Data
| Recommended Concentration | Sample | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Rat IFN-gamma Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat IFN‑ gamma Antibody (Catalog #MAB5851 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat IFN‑ gamma Biotinylated Antibody (Catalog #BAF585 ) |
| ELISA Standard | Recombinant Rat IFN-gamma Protein (Catalog #585-IF ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry IFN-gamma Antibody (88928) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IFNgamma_MAB5851_Immunocytochemistry__Immunofluorescence_19359.jpg) click image to view larger | IFN‑ gamma in Rat Splenocytes.IFN‑ gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IFN-gamma | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37% - 45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN‑ gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). | |
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References
| Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. | |
| Pestka, S. et al. (2004) Immunol. Rev. 202:8. | |
| Dijkema, R. et al. (1985) EMBO J. 4:761. | |
| Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. | |
| Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. | |
| Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. | |
| McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. | |
| Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. | |
| Kelchtermans, H. et al. (2008) Trends Immunol. 29:479. | |
| Long Name: | Interferon gamma |
| Entrez Gene IDs: | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| Alternate Names: | IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
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Related Research Areas
| Conventional/Classical Dendritic Cells | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| gamma delta T Cells | ||
| Group 1 ILCs | ||
| IL-10/Interferon Family | ||
| Infectious Diseases | ||
| Inflammatory Mediators | ||
| Isolation, Culture, and Identification of Natural Killer Cells | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Multiple Sclerosis and Myelin Related Molecules | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Natural Killer T (NKT) Cells | ||
| NCR+ILC3 | ||
| NCR-ILC3 | ||
| Neuroinflammation | ||
| Regulatory T Cells (Tregs) | ||
| T Cell Cytokine Signaling | ||
| Th1 Cells | ||
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| Immunocytochemistry | |
|---|---|
 | IFN‑ gamma in Rat Splenocytes. IFN‑ gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | IFN-gamma |
|---|---|
| background_content | Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37% - 45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN‑ gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
Stem Cells, 2008;26(4):1047-55.
Species: Rat
Sample Type: Tissue Homogenates
Application: ELISA Development - In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006;181(1):57-67.
Species: Rat
Sample Type: Plasma
Application: ELISA Development
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