抗Human IL-10抗体(Anti-Human IL-10 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-10Ser19-Asn178Accession # P22301
Source	Monoclonal Mouse IgG2B Clone # 25209
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	5 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Western Blot
	Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
	Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171). The ND50 is typically 0.005‑0.03 µg/mL.

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Related Product & Information

Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Background	IL-10
background_content	Background: IL-10 IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

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Citations

1. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
   Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
   Arthritis Res. Ther., 2017;19(1):97.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Oral fibroblasts modulate the macrophage response to bacterial challenge
   Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
   Sci Rep, 2017;7(1):11516.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
   Authors: Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E
   J Virol, 2014;88(12):6672-89.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
   Authors: Svajger U, Obermajer N, Jeras M
   J Leukoc Biol, 2014;95(1):33-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma.
   Authors: Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F
   PLoS ONE, 2014;9(6):e100909.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
   Authors: Nicolaidou V, Wong MM, Redpath AN
   PLoS ONE, 2012;7(7):e39871.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
   Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
   J. Leukoc. Biol., 2011;89(6):893-905.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
   Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
   J. Immunol., 2009;182(5):2929-38.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


9. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
    Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
    J. Immunol., 2007;179(4):2359-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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