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抗Endoglin抗体(Anti-Endoglin, Goat-Poly, Biotin antibody)

掲載日情報：2021/01/28 現在Webページ番号：29099

アールアンドディー システムス /
R&D Systems, Inc.
[メーカー略称：RSD]

Endoglinに対する抗体(Anti-Endoglin, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 ：2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Endoglin, Goat-Poly, Biotin <Anti-CD105>
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
説明文
マッチドペア：Mouse Endoglin/CD105 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB13201-500，スタンダードとして#1320-EN-025を用いる。
別名：CD105
Genbank No： 2022
Protein Accession No： Q8K100
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Biotin
交差性 Mouse 適用 ELISA,FCM,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 ：2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Endoglin, Goat-Poly, Biotin <Anti-CD105>

文献数： 2

説明文 マッチドペア：Mouse Endoglin/CD105 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB13201-500，スタンダードとして#1320-EN-025を用いる。
別名：CD105
Genbank No： 2022
Protein Accession No： Q8K100
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 ELISA,FCM,IHC,Western Blot
標識 Biotin 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityMouse
LabelBiotin
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105Glu27-Gly581Accession # Q8K100
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects mouse Endoglin/CD105 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human Endoglin is observed.


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Applications and Data

 Recommended
Concentration		Sample
Western Blot0.1 µg/mLRecombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN)
Flow Cytometry2.5 µg/106 cellsMS-1 mouse cell line
Immunohistochemistry5-15 µg/mLSee below
Mouse Endoglin/CD105 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Mouse Endoglin/CD105 Antibody (Catalog #MAB13201 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Mouse Endoglin/CD105 Biotinylated Antibody (Catalog #BAF1320 )
ELISA Standard  Recombinant Mouse Endoglin/CD105 Fc Chimera Protein, CF (Catalog #1320-EN )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry Immunohistochemistry Endoglin/CD105 Antibody [Biotin]
click image to view larger		Endoglin/CD105 in Mouse Lung.Endoglin/CD105 was detected in immersion fixed frozen sections of mouse lung using 15 µg/mL Goat Anti-Mouse Endoglin/CD105 Biotinylated Antigen Affinity-purified Poly­clonal Antibody (Catalog # BAF1320) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

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References

Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
ten Dijke, P. et al. (2008) Angiogenesis 11:79.
Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
Moody, J.L. et al. (2007) Stem Cells 25:2809.
Velasco, S. et al. (2008) J. Cell Sci. 121:913.
Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs:2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names:CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Related Research Areas

Adipose-derived Stem Cell Markers
Cancer Biomarkers
Cancer Stem Cell Markers
Endothelial Cell Markers
Hemangioblasts
Hematopoietic Stem Cell Markers
Immune Regulatory Molecules on Endothelial Cells
Mesenchymal Stem Cell Markers
Monocyte Markers
Preeclampsia
TGF-beta Family
TGF-beta Superfamily
  1. Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy.
    Authors: Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F
    PLoS ONE, 2012;7(7):e41511.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded


Immunohistochemistry
Endoglin/CD105 in Mouse Lung.
Endoglin/CD105 was detected in immersion fixed frozen sections of mouse lung using 15 µg/mL Goat Anti-Mouse Endoglin/CD105 Biotinylated Antigen Affinity-purified Poly­clonal Antibody (Catalog # BAF1320) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.


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Related Product & Information

BackgroundEndoglin/CD105
background_contentBackground:
Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Long-Term Retinal PEDF Overexpression Prevents Neovascularization in a Murine Adult Model of Retinopathy.
    Authors: Haurigot V, Villacampa P, Ribera A, Bosch A, Ramos D, Ruberte J, Bosch F
    PLoS ONE, 2012;7(7):e41511.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

  2. Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancer.
    Authors: Pimanda JE, Chan WY, Donaldson IJ, Bowen M, Green AR, Gottgens B
    Blood, 2006;107(12):4737-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC



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