抗IL-6 Receptor抗体(Anti-IL-6 Receptor, Mouse, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：29011
IL-6 Receptorに対する抗体(Anti-IL-6 Receptor, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IL-6 Receptor, Mouse, Goat-Poly |
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Anti-Mouse IL-6 R alpha Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-6 Receptor, Mouse, Goat-Poly
文献数： 16
- 商品コード：AF1830
- メーカー：RSD
- 包装：100μg
- 価格：¥107,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse IL-6 Rα サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1830，スタンダードとして#1830-SR-025を用いる。 別名：IL-6 R Genbank No： 3570 Protein Accession No： P22272 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Goat | ||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse IL-6 R alpha Affinity Purified Polyclonal Ab
文献数： 5
- 商品コード：AF1830-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 Genbank No： 3570 Protein Accession No： P22272 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha Leu20-Glu357Accession # P22272 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL‑6 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑6, recombinant human IL-6 R and rmgp130 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR) |
| Flow Cytometry | 2.5 µg/106 cells | Mouse CD3+ splenocytes |
| Immunohistochemistry | 5-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Mouse IL-6 R alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse IL‑6 R alpha Antibody (Catalog #AF1830 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑6 R alpha Biotinylated Antibody (Catalog #BAF1830 ) |
| ELISA Standard | Recombinant Mouse IL-6 R alpha (aa 20-357) Protein (Catalog #1830-SR ) | |
| Neutralization | Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunohistochemistry |
 click image to view larger | IL‑6 R alpha in Mouse Thymus.IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Immunohistochemistry |
 click image to view larger | IL‑6 R alpha in Mouse Thymus.IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Neutralization |
 click image to view larger | Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 R alpha Antibody.Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND50 is typically 0.2-1 µg/mL. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-6 R alpha | Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7). |
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References
| Yamasaki, K. et al. (1988) Science 241:825. | |
| Sugita, T. et al. (1990) J. Exp. Med. 171:2001. | |
| Hibi, M. et al. (1990) Cell 63:1149. | |
| Saito, M. et al. (1992) J. Immunol. 148:4066. | |
| Novick, D. et al. (1989) J. Exp. Med. 170:1409. | |
| Jones, S.A. et al. (2001) FASEB J. 15:43. | |
| Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251. | |
| Long Name: | Interleukin 6 Receptor alpha |
| Entrez Gene IDs: | 3570 (Human); 16194 (Mouse) |
| Alternate Names: | CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor |
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Related Research Areas
| B Cell Activation | ||
| Cancer Stem Cell Markers | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| Diabetes | ||
| IL-6 Family | ||
| Immune Regulatory Molecules on Endothelial Cells | ||
| Monocyte Markers | ||
| Multiple Sclerosis and Myelin Related Molecules | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Neuroinflammation | ||
| Obesity | ||
| Receptors in the Jak/STAT Pathway | ||
| T Cell Cytokine Signaling | ||
| Th17 Cells | ||
| Th22 Cells | ||
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| Immunohistochemistry | |
|---|---|
 | IL‑6 R alpha in Mouse Thymus. IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Immunohistochemistry | |
 | IL‑6 R alpha in Mouse Thymus. IL‑6 R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Neutralization | |
 | Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 R alpha Antibody. Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND50 is typically 0.2-1 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 6 Receptor alpha |
|---|---|
| Entrez Gene IDs | 3570 (Human); 16194 (Mouse) |
| Background | IL-6 R alpha |
| background_content | Background: IL-6 R alpha Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling
Authors: P Arnold, I Boll, M Rothaug, N Schumacher, F Schmidt, R Wichert, J Schneppenh, J Lokau, U Pickhinke, T Koudelka, A Tholey, B Rabe, J Scheller, R Lucius, C Garbers, S Rose-John, C Becker-Pau
Sci Rep, 2017;7(0):44053. - Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130
Authors: X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns
J Neuroinflammation, 2017;14(1):121.
Species: Mouse
Sample Type: In Vivo
Application: Neut - Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner
Authors: Nana Owusu-Boai
Immun Inflamm Dis, 2016;4(4):413-426.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay - Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding.
Authors: Garbers C, Janner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J
J. Biol. Chem., 2011;286(17):14804-11.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: WB - IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice.
Authors: Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW
Blood, 2007;111(8):3978-85.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: WB - Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007;110(6):1748-55.
Species: Mouse
Sample Type: Lavage Fluid
Application: ELISA Development - Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood, 2007;111(3):1021-8.
Species: Mouse
Sample Type: Air Pouch Exudate
Application: ELISA Development - Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
Authors: Kurooka M, Kaneda Y
Cancer Res., 2007;67(1):227-36.
Species: Mouse
Sample Type:
Application: In vivo - Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Type: Plasma
Application: ELISA Development
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