抗IL-6抗体(Anti-IL-6, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-6Phe25-Thr211Accession # P08505
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL‑6 and recombinant cotton rat IL-6 is observed, 5% cross-reactivity with recombinant human IL-6, recombinant porcine IL-6, recombinant canine IL‑6, and recombinant feline IL‑6 is observed, and less than 1% cross-reactivity with recombinant equine IL-6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	Immersion fixed mouse splenocytes treated with PMA and ionomycin
Neutralization	Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Western Blot
	Detection of Mouse IL‑6 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
	Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND50 is typically0.01-0.03 µg/mL.

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Related Product & Information

Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
Background	IL-6
background_content	Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).

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Citations

1. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
   Authors: C Dempsey
   Brain Behav. Immun, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


2. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
   Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
   Sci Rep, 2016;6(0):19404.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
   J Leukoc Biol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.
   Authors: Rudraraju R, Jones B, Surman S, Sealy R, Thomas P, Hurwitz J
   PLoS ONE, 2014;9(1):e86554.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
   Authors: Uto-Konomi A, Miyauchi K, Ozaki N
   PLoS ONE, 2012;7(7):e40343.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
   Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
   J. Clin. Invest., 2011;121(5):1692-708.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
   Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
   J. Clin. Invest., 2011;121(10):3846-59.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Foxa1 and Foxa2 regulate bile duct development in mice.
   Authors: Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH
   J. Clin. Invest., 2009;119(6):1537-45.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
   Authors: Weng TI, Wu HY, Lin PY, Liu SH
   Infect. Immun., 2009;77(8):3312-9.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


10. The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S
    Eur. J. Pharmacol., 2008;592(1):87-92.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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