抗IL-1β抗体(Anti-IL-1β, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-1 beta /IL-1F2Val118-Ser269Accession # NP_032387
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL‑1 beta and recombinant porcine IL-1 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Immunocytochemistry
	IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL‑1 beta /IL‑1F2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot
	Detection of Human and MouseIL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-)ortreated (+) with 200 nM PMA for 24 hoursand 10 μg/mL LPS for 4 hours andRAW 264.7 mouse monocyte/macrophagecell line untreated (-)or treated (+) with10 μg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	IL‑1 beta /IL‑1F2 in Mouse Thymus. IL‑1 beta /IL‑1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization
	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by MouseIL-1 beta /IL-1F2 Antibody. Recombinant MouseIL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50 is typically ≤0.25 µg/mL.

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Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.

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Citations

1. MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration
   Authors: E Lee, I Hwang, S Park, S Hong, B Hwang, Y Cho, J Son, JW Yu
   Cell Death Differ., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages
   Authors: LH Chu, M Indramohan, RA Ratsimandr, A Gangopadhy, EP Morris, DM Monack, A Dorfleutne, C Stehlik
   Nat Commun, 2018;9(1):996.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation
   Authors: RG Carroll, Z Zas?ona, S Galván-Peñ, EL Koppe, DC Sévin, S Angiari, M Triantafil, K Triantafil, LK Modis, LA O'Neill
   J. Biol. Chem., 2018;293(15):5509-5521.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


4. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation
   Authors: SB Kwak, S Koppula, EJ In, X Sun, YK Kim, MK Kim, KH Lee, TB Kang
   Mediators Inflamm., 2018;2018(0):6054069.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


5. HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
   Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
   PLoS ONE, 2018;13(3):e0195335.
   Species: Mouse
   Sample Type: Protein
   Application: WB


6. Inhibitory Effect and Mechanism ofArctium lappaExtracton NLRP3Inflammasome Activation
   Authors: YK Kim, S Koppula, DW Shim, EJ In, SB Kwak, MK Kim, SH Yu, KH Lee, TB Kang
   Evid Based Complement Alternat Med, 2018;2018(0):6346734.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Tranilast directly targets NLRP3 to treat inflammasome-driven diseases
   Authors: Y Huang, H Jiang, Y Chen, X Wang, Y Yang, J Tao, X Deng, G Liang, H Zhang, W Jiang, R Zhou
   EMBO Mol Med, 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1?
   Authors: Q Zhu, TD Kanneganti
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes
   Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba
   J. Biol. Chem., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. MTG16 is a tumor suppressor in colitis-associated carcinoma
    Authors: EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams
    JCI Insight, 2017;2(16):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

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