抗LAP抗体(Anti-LAP, TGF-β1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1Leu30-Ser390Accession # P01137
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human LAP TGF‑ beta 1 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	Immersion fixed human peripheral blood mononuclear cells
Neutralization	Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.4-2 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL TGF‑ beta 1.

Western Blot
	Detection of Human LAP (TGF-beta 1) by Western Blot. Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human LAP (TGF-beta 1) by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LAP (TGF-beta 1) at approximately 61 kDa (as indicated) using 20 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-246-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization
	LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF‑ beta 1 (Catalog # 246‑LP) inhibits Recom­binant Human TGF‑ beta 1 (Catalog # 240‑B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recom­binant Human TGF‑ beta 1 (1 ng/mL) activity elicited by Recombin­ant Human LAP TGF‑ beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND50 is typically 0.4‑2 µg/mL.

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Related Product & Information

Long Name	Latency-associated Peptide
Entrez Gene IDs	7040 (Human)
Background	LAP (TGF-beta 1)
background_content	Background: LAP (TGF-beta 1) TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF‑ beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-beta 1 (aa 279-390) (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF‑ beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5-7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF‑ beta 1 (10).

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Citations

1. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Cutting Edge: ERK1 Mediates the Autocrine Positive Feedback Loop of TGF-? and Furin in Glioma-Initiating Cells
   Authors: E Ventura, M Weller, I Burghardt
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration
   Authors: O Miroshnych, WT Chang, JL Dragoo
   Am J Sports Med, 2017;45(4):945-953.
   Species: Human
   Sample Type: Protein
   Application: WB


4. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells
   Authors: Boris Hinz
   Nat Mater, 2016;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


5. Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells.
   Authors: Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O
   Mol Oncol, 2014;8(1):68-82.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


6. Identification of bone morphogenetic protein 7 (BMP7) as an instructive factor for human epidermal Langerhans cell differentiation.
   Authors: Yasmin N, Bauer T, Modak M, Wagner K, Schuster C, Koffel R, Seyerl M, Stockl J, Elbe-Burger A, Graf D, Strobl H
   J Exp Med, 2013;210(12):2597-610.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells.
   Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F
   Cancer Res, 2013;73(7):2333-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche.
   Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H
   Cell, 2011;147(5):1146-58.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
   Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
   Exp. Cell Res., 2011;317(7):976-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis.
    Authors: Ha CT, Wu JA, Irmak S, Lisboa FA, Dizon AM, Warren JW, Ergun S, Dveksler GS
    Biol. Reprod., 2010;83(1):27-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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