抗Human IL-4 Phycoerythrin抗体(Anti-Human IL-4 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL‑4His25-Ser153Accession # P05112.1
Source	Monoclonal Mouse IgG1 Clone # 3007
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑4.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑4 in NS0 Mouse Cell Line Transfected with Human IL-4 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-4 was stained with Mouse Anti-Human IL‑4 PE‑conjugated Monoclonal Antibody (Catalog # IC204P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-4

background_content

Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the Common gamma  Chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

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Citations

1. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
   Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
   J Allergy Clin Immunol, 2014;133(1):172-80.e1-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Chip Cytometry


2. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
   Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
   J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
   Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
   Cancer Res., 2007;67(20):9630-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
   Authors: D'Ambrosio D
   Eur. J. Immunol., 2002;32(10):2934-43.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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