抗Mouse IFN-gamma抗体(Anti-Mouse IFN-gamma antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IFN-gamma
Source	Monoclonal Rat IgG2A and IgG2B Cocktail Clone # 37801/37875
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IFN-gamma in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) C10, rmG-CSF, rmGM‑CSF, recombinant human IFN‑ gamma, rmIL-1 alpha, rmIL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmIL-9, rmIL-10, rmIL-10 R, rmIL-12, rmIL-13, rmJE, rmKC, rmLIF, rmM-CSF, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-2, rmSCF, rmTNF-alpha, rmTpo, and rmVEGF.

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Applications and Data

	Recommended Concentration	Sample
Mouse IFN-gamma Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IFN‑ gamma Antibody (Catalog #MAB785 )
ELISA Detection (Matched Antibody Pair)	0.4-0.8 µg/mL	Mouse IFN‑ gamma Biotinylated Antibody (Catalog #BAF485 )
ELISA Standard		Recombinant Mouse IFN-gamma Protein (Catalog #485-MI )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38% - 44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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References

Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
Gray, P.W. and D.V. Goeddel (1983) Proc. Natl. Acad. Sci. 80:5842.
Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name:	Interferon gamma
Entrez Gene IDs:	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:	IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Related Research Areas

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Group 1 ILCs

IL-10/Interferon Family

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Macrophage Activation Markers

Molecules Secreted by VSMC

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

NCR+ILC3

NCR-ILC3

Neuroinflammation

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge Authors: O Martínez, A Bravo Cruz, S Santos, M Ramírez, E Miranda, J Shisler, M Otero Vaccine, 2017;0(0):. Species: Mouse Sample Type: Whole Cells Application: ELISpot Capture

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Related Product & Information

Background

IFN-gamma

background_content

Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38% - 44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge
   Authors: O Martínez, A Bravo Cruz, S Santos, M Ramírez, E Miranda, J Shisler, M Otero
   Vaccine, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISpot Capture


2. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
   Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
   J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


3. Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex
   Authors: I Hwang, K Kim, S Choi, M Lomunova
   Mol. Cells, 2017;40(1):24-36.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ELISA Capture


4. Tenascin-C deficiency protects mice from experimental autoimmune encephalomyelitis
   Authors: Miljana Mom?ilovi?
   J. Neuroimmunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


5. Immunomodulator-based enhancement of anti smallpox immune responses.
   Authors: Martinez O, Miranda E, Ramirez M, Santos S, Rivera C, Vazquez L, Sanchez T, Tremblay R, Rios-Olivares E, Otero M
   PLoS ONE, 2015;10(4):e0123113.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Capture


6. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
   Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
   Mucosal Immunol, 2014;7(2):417-27.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Capture


7. An alphavirus-based adjuvant enhances serum and mucosal antibodies, T cells, and protective immunity to influenza virus in neonatal mice.
   Authors: Khalil S, Tonkin D, Snead A, Parks G, Johnston R, White L
   J Virol, 2014;88(16):9182-96.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Capture


8. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
   Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
   Eur. J. Immunol., 2012;42(5):1250-60.
   Species: Mouse
   Sample Type: Pleural Lavage Fluid
   Application: ELISA Development


9. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
   Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
   Vaccine, 2010;28(8):1942-51.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISpot Development


10. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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