抗Mouse IL-2抗体(Anti-Mouse IL-2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-2
Source	Monoclonal Rat IgG1 Clone # JES6-1A12
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-2 in ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Mouse IL-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑2 Antibody (Catalog #MAB702 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑2 Biotinylated Antibody (Catalog #BAF402 )
ELISA Standard		Recombinant Mouse IL-2 Protein (Catalog #402-ML )
Neutralization	Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.125-0.75 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2	IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma  chains.
Long Name:	Interleukin 2
Entrez Gene IDs:	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:	aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

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Related Research Areas

Cancer Biomarkers

Common gamma Chain Receptor Family

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Group 1 ILCs

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Multiple Sclerosis and Myelin Related Molecules

NCR+ILC3

Neuroinflammation

Preeclampsia

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Th2 Cells

Th9 Cells

Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression. Authors: Simonetta F, Gestermann N, Bloquet S, Bourgeois C PLoS ONE, 2014;9(12):e113314. Species: Mouse Sample Type: In Vivo Application: Adoptive Transfer

Neutralization

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.

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Related Product & Information

Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Background	IL-2
background_content	Background: IL-2 IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma  chains.

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Citations

1. Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression.
   Authors: Simonetta F, Gestermann N, Bloquet S, Bourgeois C
   PLoS ONE, 2014;9(12):e113314.
   Species: Mouse
   Sample Type: In Vivo
   Application: Adoptive Transfer


2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


3. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
   Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
   FASEB J., 2011;25(1):409-19.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
   Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
   J. Immunol., 2010;184(4):2130-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.
   Authors: Ito H, Ando K, Ishikawa T
   Int. Immunol., 2008;20(7):869-79.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Tumor-specific Th17-polarized cells eradicate large established melanoma.
   Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
   Blood, 2008;112(2):362-73.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
   Authors: Bednarski JJ, Warner RE, Rao T
   Arthritis Rheum., 2003;48(3):757-66.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISpot Development

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