抗Human IL-2抗体(Anti-Human IL-2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Primate
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-2Ala21-Thr153Accession # NP_000577
Source	Monoclonal Mouse IgG2A Clone # 5355
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-3, rhIL-4, rhIL‑6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below
Human/Primate IL-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Primate IL‑2 Antibody (Catalog #MAB602 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human/Primate IL‑2 Biotinylated Antibody (Catalog #BAF202 )
ELISA Standard		Recombinant Human IL-2 Protein (Catalog #202-IL )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	IL‑2 in Human PBMCs.IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2	Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.
Long Name:	Interleukin 2
Entrez Gene IDs:	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:	aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

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Related Research Areas

Cancer Biomarkers

Common gamma Chain Receptor Family

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Group 1 ILCs

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Multiple Sclerosis and Myelin Related Molecules

NCR+ILC3

Neuroinflammation

Preeclampsia

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th1 Cells

Th2 Cells

Th9 Cells

Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter J. Immunol, 2017;0(0):. Species: Human Sample Type: Cell Culture Supernates

Immunocytochemistry

IL‑2 in Human PBMCs. IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background	IL-2
background_content	Background: IL-2 Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

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Citations

1. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
   Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Cell Culture Supernates
   


2. Key Residues at Third CDR3? Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs
   Authors: Kenji Chamoto
   J. Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: ELISA Capture


3. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
   Authors: Rochman Y, Yukawa M, Kartashov A, Barski A
   PLoS ONE, 2015;10(4):e0122198.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


4. Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'.
   Authors: Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
   Nature, 2012;484(7395):529-33.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Neut


5. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
   Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
   Blood, 2008;112(9):3704-12.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Generation of Cytokine


6. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


7. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


8. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
   Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
   AIDS, 2007;21(10):1263-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


9. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
   Authors: Pfleger C, Schloot N, ter Veld F
   J. Immunol. Methods, 2007;329(1):214-8.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


10. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development

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