抗Mouse CD3 epsilon抗体(Anti-Mouse CD3 epsilon antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	H-2Kb-specific mouse cytotoxic T-lymphocyte
Source	Monoclonal Hamster IgG Clone # 145-2C11
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Mouse splenocytes
Immunoprecipitation	Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.
Cell depletion	Small, M. et al. (1994) J. Immunol. Meth. 167:103.
CyTOF-reported	Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
T Cell Stimulation	0.1-0.6 µg/mL	See below

T Cell Stimulation
	Mouse CD3 epsilon  Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1‑0.6 μg/mL.

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Related Product & Information

Entrez Gene IDs	916 (Human); 12501 (Mouse)
Background	CD3 epsilon
background_content	Background: CD3 epsilon CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7

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Citations

1. Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking
   Authors: T Savinko, C Guenther, LM Uotila, M Llort Asen, S Yao, S Tojkander, SC Fagerholm
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


2. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
   Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
   PLoS Pathog, 2017;13(1):e1006120.
   


3. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis
   Authors: LT McDonald, SD Johnson, DL Russell, MRI Young, AC LaRue
   PLoS ONE, 2017;12(7):e0180724.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


4. mTORC2 controls Th9 polarization and allergic airway inflammation
   Authors: H Chen, L Zhang, P Wang, H Su, W Wang, Z Chu, L Zhang, X Zhang, Y Zhao
   Allergy, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


5. Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration.
   Authors: Gupta S, Marcel N, Talwar S, Garg M, R I, Perumalsamy L, Sarin A, Shivashankar G
   PLoS ONE, 2012;7(9):e43718.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


6. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
   Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
   FASEB J., 2011;25(1):409-19.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


7. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
   Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
   J. Immunol., 2010;184(4):2130-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
   Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
   Nat. Immunol., 2008;9(5):513-21.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


9. Protection of CD8+ T cells from activation-induced cell death by IL-18.
   Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
   J. Leukoc. Biol., 2007;82(1):142-51.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


10. Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
    Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
    J. Immunol., 2006;177(8):5041-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay

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