抗IL-5抗体(Anti-IL-5, Mouse, Rat-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Mouse T cell clone-derived partially purified recombinant mouse IL-5
Source	Monoclonal Rat IgG2A Clone # TRFK4
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-5, recombinant mouse (rm) IL‑3, or rmGM-CSF is observed.

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Applications and Data

	Recommended Concentration	Sample
Mouse IL-5 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Mouse IL‑5 Antibody (Catalog #MAB405R )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Mouse IL‑5 Biotinylated Antibody (Catalog #BAM705 )
ELISA Standard		Recombinant Mouse IL-5 Protein (Catalog #405-ML )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5	IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
Long Name:	Interleukin 5
Entrez Gene IDs:	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names:	BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Related Research Areas

Common beta Chain Receptor Family

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Tfh Cell Differentiation and Function

gamma delta T Cells

Granulocyte Markers

Group 2 ILCs

Inflammatory Mediators

T Cell Cytokine Signaling

Th2 Cells

Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3. Species: Mouse Sample Type: Serum Application: ELISA detection

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Related Product & Information

Background	IL-5
background_content	Background: IL-5 IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

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Citations

1. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
   Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
   J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


3. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
   Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
   Am. J. Pathol., 2006;168(6):1940-50.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


4. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
   Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
   Toxicology, 2005;206(2):181-93.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
   Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
   Am. J. Pathol., 2002;160(4):1353-60.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development

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